Growth of embryonic avian and mammalian tibiae on a relatively simple chemically defined medium

Biggers, John D.; Gwatkin, Ralph B. L.; Heyner, Susan

doi:10.1016/0014-4827(61)90305-6

Cited by 246 publications

(58 citation statements)

References 12 publications

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“…Selected clinical data on the 12 patients whose bone marrow leukocytes were cultured are given in Table I. Cells were obtained by direct bone-marrow aspiration, washed six times in a balanced salt solution, and cultured at a concentration of 1-2 x 106 cell/ml (5 (14) supplemented with 10% heat-inactivated rat serum. Supernatant fluids were harvested after 24 h of culture.…”

Section: Methodsmentioning

confidence: 99%

Observations on the Mechanism of Bone Resorption Induced by Multiple Myeloma Marrow Culture Fluids and Partially Purified Osteoclast-activating Factor

Josse¹,

Murray²,

Mundy³

et al. 1981

J. Clin. Invest.

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A B S T R A C T Supernatant fluids from the cultures of bone marrow cells from 10 of 12 patients with multiple myeloma (MM) caused bone resorption in organ cultures of fetal rat calvaria. In four patients, the marrow cells were cultured with and without indomethacin (1 ,uM). The supernatant fluids from indomethacintreated marrow cultures caused significantly less bone resorption than supernatant fluids of cell cultures without indomethacin. This inhibition of release of bone resorbing factor(s) by myeloma cultures is similar to the previously observed indomethacin-induced inhibition of osteoclast-activating factor (OAF) production by activated human leukocytes. None of the MM supernatants had any effect on cyclic (c)AMP accumulation in resorbing bone in vitro.Four separate preparations of partially purified OAF obtained from phytohemagglutinin-stimulated peripheral human leukocytes were tested for their ability (a) to cause bone resorption in organ cultures of fetal rat and neonatal mouse calvaria and (b) to cause accumulation of cAMP in rat and mouse skeletal tissue in vitro. Those dilutions of OAF that caused bone resorption had no effect on accumulation ofcAMP in rat or mouse calvaria incubated in vitro. In addition, no stimulation of adenylate cyclase activity in membranes prepared from fetal rat calvaria could be found. Bone cell populations isolated by sequential collagenase digestion of fetal rat calvaria also showed no cAMP response to these dilutions of OAF The resorptive action of MM culture fluids is similar to that of partially purified OAF from activated cultured leukocytes, but different from those of other bone resorbing factors, parathyroid hormone and prostaglandin E2, which stimulate cAMP production in skeletal tissue.

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Section: Methodsmentioning

confidence: 99%

Observations on the Mechanism of Bone Resorption Induced by Multiple Myeloma Marrow Culture Fluids and Partially Purified Osteoclast-activating Factor

Josse¹,

Murray²,

Mundy³

et al. 1981

J. Clin. Invest.

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“…BGJ culture medium (Biggers, Gwatkin and Heynor, 1961) was prepared as a powder. The final solution was suppleniented with antibiotics, an antifungal agent, ascorbic acid (150 ,ug/ml) and 15% heated horse serum as described previously (Webster, Atkins and Peacock, 1974).…”

Section: Methodsmentioning

confidence: 99%

Secretion of prostaglandins as bone-resorbing agents by renal cortical carcinoma in culture

Atkins¹,

Ibbotson²,

Hillier³

et al. 1977

Br J Cancer

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Summary.-Fragments of human renal carcinoma tissue have been co-cultured with mouse calvaria. In 9/13 cases significant bone resorption occurred whilst in no case did control kidney cause significant resorption. When bone resorption did occur, it could be reduced by inclusion of indomethacin in the culture medium. In some cases when theophylline was included in culture medium to prevent cyclic AMP breakdown, there was enhancement of tumour-induced bone resorption. Control studies without tumour showed that none of the experimental treatments had a direct effect on bone. Radioimmunoassay of prostaglandin E (PGE) levels in pooled culture media showed that tumour fragments produced appreciable amounts of PGE, and that this production was lowered by indomethacin and increased by theophylline. It is concluded that the bone resorption induced by these tumours is due to a prostaglandin, and that prostaglandin production may be controlled by changes in cyclic AMP metabolism.

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“…The buffy coat was separated from the erythrocytes, and the leukocytes were counted in a Neubauer counting chamber. The leukocytes were diluted in fresh BGJ medium (chemically defined medium developed by Biggers et al [1]) without added proteins (8) to a concentration of 1-2 x 106 cells/ml. The method of cell culture was essentially similar to that described before (4), except that the cells were cultured in 1,000-3,000-ml Ehrlenmyer flasks, with 500 ml of culture medium in each flask.…”

Section: Introductionmentioning

confidence: 99%

Big and Little Forms of Osteoclast Activating Factor

Mundy¹,

Raisz²,

Shapiro³

et al. 1977

J. Clin. Invest.

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Growth of embryonic avian and mammalian tibiae on a relatively simple chemically defined medium

Cited by 246 publications

References 12 publications

Observations on the Mechanism of Bone Resorption Induced by Multiple Myeloma Marrow Culture Fluids and Partially Purified Osteoclast-activating Factor

Observations on the Mechanism of Bone Resorption Induced by Multiple Myeloma Marrow Culture Fluids and Partially Purified Osteoclast-activating Factor

Secretion of prostaglandins as bone-resorbing agents by renal cortical carcinoma in culture

Big and Little Forms of Osteoclast Activating Factor

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