“…To elucidate the optimum nutrient concentrations for specific growth rates of the two species, we conducted experiments under a wide range of nutrient concentrations. Water temperature, salinity, and irradiance for the experiments were fixed at three temperatures (20, 24, and 28 8C), 30 salinity, and 600 mmol m À2 s À1 , respectively, which were optimum conditions for the two species being cultured (Baek et al, 2006(Baek et al, , 2008. Based on T 1 medium seawater (N:P = 10:1, 1 ml stock solution per one liter filtered seawater), nutrient concentrations were changed to control (T 0 ), T 1 , T 2 , T 5 , T 10 , T 20 , and T 50 , which were made by the addition of no nutrients, 1 ml, 2 ml, 5 ml, 10 ml, 20 ml, and 50 ml of nutrient stock solution per 1 l of filtered seawater, respectively.…”