Growth of dimorphic human pathogenicfungi on media containing cycloheximide and chloramphenicol

Donough, E. S. Mc; Georg, Lucille K.; Ajello, Libero; Brinkman, Sherry A.

doi:10.1007/bf02155508

Cited by 17 publications

(4 citation statements)

References 7 publications

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“…The addition of chloramphenicol to the experimental con- version media inhibited the growth of the yeast form at 37°C, confirming previously reported findings by McDonough et al (15,16). Other antimicrobial agents did not interfere with yeast growth at 37°C within 2 weeks.…”

supporting

confidence: 89%

Conversion of Blastomyces dermatitidis to the yeast form at 37 degrees C and 26 degrees C

Kane¹

1984

J Clin Microbiol

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supporting

confidence: 89%

Conversion of Blastomyces dermatitidis to the yeast form at 37 degrees C and 26 degrees C

Kane¹

1984

J Clin Microbiol

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“…The concentration ranges of neomycin sulfate and chloramphenicol concentrations were extended to increase the possibility of finding optimal concentrations. Sodium azide and glycerol were added to enhance the antifungal activity and viscosity, and to mitigate the hemolysis in storage media (Table S1) [13,14].…”

Section: Screening Of Affecting Factorsmentioning

confidence: 99%

Optimization of Storage Medium for Hematological Reference Samples in External Quality Assessment

Tran

Truong

et al. 2021

Applied Sciences

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Hematological reference samples for external quality assessment (EQA) in hematology are essential for many laboratories in Vietnam, however, the prices are high along with short storing time. This study attempted to establish an optimal formula for the manufacture of hematological reference samples for hematological EQA programs. Human red blood cells (RBCs) were mixed with goose RBCs (pseudo-leucocytes) and goat RBCs (pseudo-platelets) as alternatives for white blood cells (WBCs) and platelets (PLTs), respectively, in different formulas of storage media. The optimal formula was obtained using response surface methodology–central composite design (RSM-CCD). Three main factors affecting the concentrations of RBCs were determined by Plackett–Burmann matrix. The optimal concentrations determined by RSM-CCD were 46% serum, 0.44 g/L neomycin sulfate, and 2.5% glycerol. The model predicted that the maximum number of human RBCs was 4.1 × 1012 cells/L, pseudo-leucocytes was 6.4 × 109 cells/L, and pseudo-platelets was 188 × 109 cells/L. The practical concentrations of RBCs, pseudo-leucocytes, and pseudo-platelets were 4.21 × 1012 cells/L, 6.4 × 109 cells/L, and 187 × 109 cells/L, respectively, which showed 98.93% similarity with theoretical data. This result could be a premise for further study to improve manufacturing of hematological reference samples for hematology EQA programs.

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“…11 The culture-based methods available currently are designed to isolate the mold form of the fungus, as the yeast form is highly sensitive to both cycloheximide and chloramphenicol, which are used to inhibit the fast growing molds and bacteria present in clinical specimens. 12 Exposure to microconidia during culture thus poses a biohazard risk to laboratory workers.…”

mentioning

confidence: 99%

Histologic and molecular identification of disseminated Histoplasma capsulatum in a captive brown bear (Ursus arctos)

et al. 2011

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A 33-year-old brown bear (Ursus arctos) was evaluated for chronic cough, partial anorexia, and lethargy in early fall of 2009. Radiographs revealed a generalized increase in interstitial density with focal lung field consolidation and air bronchograms more prevalent in the cranial lung lobes. Tracheal sputum and wash fluid grew mixed bacteria and 2 species of Candida on bacterial and fungal cultures, respectively. Serum was negative for antibodies to Aspergillus, Blastomyces, Coccidioides, and Histoplasma by semiquantitative radial immunodiffusion. Antimicrobial and antifungal treatment was administered. The bear died 1 month after entering hibernation. Gross necropsy revealed coalescent nodules and sheets of firm tan tissue covering pleural surfaces of the thoracic cavity and within pulmonary parenchyma, enlarged mesenteric lymph nodes, and intestinal ulcerations. Histopathology revealed granulomatous inflammation with intrahistiocytic yeast, consistent with Histoplasma organisms, in lung, diaphragm, mesenteric lymph nodes, intestine, and adrenal glands. Molecular analysis performed on DNA isolated from lung tissue, including conventional polymerase chain reaction (PCR) targeting the internal transcribed spacer region for the ribosomal RNA gene complex and real-time PCR targeting the gene encoding a unique region of M specific protein, identified the organism to be 100% identical to Histoplasma capsulatum with an average of 4.9 × 10(7) gene copies per gram of tissue. The present report describes histologic and molecular techniques for diagnosing histoplasmosis.

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Growth of dimorphic human pathogenicfungi on media containing cycloheximide and chloramphenicol

Cited by 17 publications

References 7 publications

Conversion of Blastomyces dermatitidis to the yeast form at 37 degrees C and 26 degrees C

Conversion of Blastomyces dermatitidis to the yeast form at 37 degrees C and 26 degrees C

Optimization of Storage Medium for Hematological Reference Samples in External Quality Assessment

Histologic and molecular identification of disseminated Histoplasma capsulatum in a captive brown bear (Ursus arctos)

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