Growth of cholesteatoma by implantation of epithelial tissue along the femoral bone of rats

Magalhaes, Sandra; Reforme, Olga Maria Rojas; Guzmán, Raquel Liriano; Fukuda, Yotaka; Barbosa, Flavia L.

doi:10.1016/s1808-8694(15)31309-4

Cited by 3 publications

(4 citation statements)

References 10 publications

(6 reference statements)

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“…In future research, we plan to explore these design options and evaluate the imaging system in vivo in an animal model. We are able to induce cholesteatoma 50 , 51 in gerbils by ligating their Eustachian tubes. Since blood is always prevalent in any surgical setting, there is a likelihood that the autofluorescence signal of the cholesteatoma will be diminished by light absorption at 545 and 580 nm 52 .…”

Section: Discussionmentioning

confidence: 99%

Imaging guidance for cholesteatoma surgery using tissue autofluorescence

Yang

Farrell

et al. 2023

J. Biomed. Opt.

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.SignificanceCholesteatoma is an expansile destructive lesion of the middle ear and mastoid, which can result in significant complications by eroding adjacent bony structures. Currently, there is an inability to accurately distinguish cholesteatoma tissue margins from middle ear mucosa tissue, causing a high recidivism rate. Accurately differentiating cholesteatoma and mucosa will enable a more complete removal of the tissue.AimDevelop an imaging system to enhance the visibility of cholesteatoma tissue and margins during surgery.ApproachCholesteatoma and mucosa tissue samples were excised from the inner ear of patients and illuminated with 405, 450, and 520 nm narrowband lights. Measurements were made with a spectroradiometer equipped with a series of different longpass filters. Images were obtained using a red-green-blue (RGB) digital camera equipped with a long pass filter to block reflected light.ResultsCholesteatoma tissue fluoresced under 405 and 450 nm illumination. Middle ear mucosa tissue did not fluoresce under the same illumination and measurement conditions. All measurements were negligible under 520 nm illumination conditions. All spectroradiometric measurements of cholesteatoma tissue fluorescence can be predicted by a linear combination of emissions from keratin and flavin adenine dinucleotide. We built a prototype of a fluorescence imaging system using a 495 nm longpass filter in combination with an RGB camera. The system was used to capture calibrated digital camera images of cholesteatoma and mucosa tissue samples. The results confirm that cholesteatoma emits light when it is illuminated with 405 and 450 nm, whereas mucosa tissue does not.ConclusionsWe prototyped an imaging system that is capable of measuring cholesteatoma tissue autofluorescence.

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Section: Discussionmentioning

confidence: 99%

Imaging guidance for cholesteatoma surgery using tissue autofluorescence

Yang

Farrell

et al. 2023

J. Biomed. Opt.

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show abstract

“…In future research, we plan to explore these design options and evaluate the imaging system in vivo in an animal model. We are able to induce cholesteatoma 47,48 in gerbils by ligating their Eustachian tubes. Since blood is always prevalent in any surgical setting, there is a likelihood that the autofluorescence signal of the cholesteatoma will be diminished by light absorption at 545 and 580 nm.…”

Section: Discussionmentioning

confidence: 99%

Novel Imaging Guidance for Cholesteatoma Surgery using Tissue Autofluorescence

Yang¹,

Farrell

Ye³

et al. 2023

Preprint

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Significance: Cholesteatoma is an expansile destructive lesion of the middle ear and mastoid, which can result in significant complications by eroding adjacent bony structures. Currently, there is an inability to accurately distinguish cholesteatoma tissue margins from middle ear mucosa tissue, causing a high recidivism rate. Accurately differentiating cholesteatoma and mucosa will enable a more complete removal of the tissue. Aim: Develop an imaging system to enhance the visibility of cholesteatoma tissue and margins during surgery. Approach: Cholesteatoma and mucosa tissue samples were excised from the inner ear of patients and illuminated with 405, 450, and 520 nm narrowband lights. Measurements were made with a spectroradiometer equipped with a series of different longpass filters. Images were obtained using a red-green-blue (RGB) digital camera equipped with a long pass filter to block reflected light. Results: Cholesteatoma tissue fluoresced under 405 and 450 nm illumination. Middle ear mucosa tissue did not fluoresce under the same illumination and measurement conditions. All measurements were negligible under 520 nm illumination conditions. All spectroradiometric measurements of cholesteatoma tissue fluorescence can be predicted by a linear combination of emissions from keratin and flavin adenine dinucleotide (FAD). We built a prototype of a fluorescence imaging system using a 495 nm longpass filter in combination with an RGB camera. The system was used to capture calibrated digital camera images of cholesteatoma and mucosa tissue samples. The results confirm that cholesteatoma emits light when it is illuminated with 405 and 450 nm, whereas mucosa tissue does not. Conclusions: We prototyped an imaging system that is capable of measuring cholesteatoma tissue autofluorescence.

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“…Technically, many modeling methods have been reported to successfully induce cholesteatoma including the ligation of the external ear canal [5,16], blocking of the eustachian tube [17,18], injection of chemical or biological material into the middle ear [19], autologous dermal implantation [2,20,21], and local hybrid ear [6]. However, in the present study we implanted autologous meatal skin grafts into the middle ear via the tympanic membrane followed by an intratympanic injection of PA to experimentally induce middle ear cholesteatoma in a mouse model.…”

Section: Discussionmentioning

confidence: 99%

“…Chole et al [20] described a cholesteatoma-related bone resorption model after implanting keratin particles onto the calvarium of mice. Magalhaes et al [21] reported that they implanted auricular skin on the femoral bone of rats and thereby successfully induced cholesteatoma. However, neither is an experimental model of acquired middle ear cholesteatoma.…”

Section: Discussionmentioning

confidence: 99%

Autologous Meatal Skin Graft Implantation and Intratympanic Injection of <b><i>Pseudomonas aeruginosa</i></b>: A New Experimental Mouse Model of Acquired Middle Ear Cholesteatoma

Chen

et al. 2013

ORL

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Aim: To create an experimental model for the biomedical research of middle ear cholesteatoma. Methods: Cholesteatoma was induced in the right ears of mice. An autologous meatal skin graft was implanted into the middle ear via the tympanic membrane followed by an intratympanic injection of Pseudomonas aeruginosa. Six weeks after surgery, the formation of acquired cholesteatoma was evaluated by macroscopic examination, CT scan, and histological analysis. The expressions of TNF-α, IL-1β, and IL-6 were measured with real-time PCR. Auditory-evoked brain stem response was used for assessing the changes in hearing levels. Results: None of the mice died during the modeling time. By the sixth postoperative week, cholesteatoma had successfully formed in 23 out of 25 mice, which equals a success rate of 92%. A CT scan revealed that the cholesteatoma was eroding the cochlea. Furthermore, histological analysis demonstrated a cystic structure covered by stratified squamous epithelium and keratin desquamation in the lamellae inside the cystic cavity in the bullae. All mice with experimentally induced cholesteatoma showed hearing impairment and an upregulated expression of TNF-α, IL-1β, and IL-6. Conclusion: The present study successfully developed a mouse model of acquired middle ear cholesteatoma, which provides an extremely valuable tool for auditory and biomedical research. The modeling approach is technically easy and has a high success rate.

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Growth of cholesteatoma by implantation of epithelial tissue along the femoral bone of rats

Abstract: Growth of epidermal cyst (cholesteatoma) may start from a transplanted epithelial tissue next to the femoral bone of rats.

Cited by 3 publications

References 10 publications

Imaging guidance for cholesteatoma surgery using tissue autofluorescence

Imaging guidance for cholesteatoma surgery using tissue autofluorescence

Novel Imaging Guidance for Cholesteatoma Surgery using Tissue Autofluorescence

Autologous Meatal Skin Graft Implantation and Intratympanic Injection of <b><i>Pseudomonas aeruginosa</i></b>: A New Experimental Mouse Model of Acquired Middle Ear Cholesteatoma

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