Growth of Chicken-Embryo-Lethal-Orphan (CELO) Virus in Chicken Embryo Neuroglial and Chicken Embryo Fibroblastic Cell Cultures

Mancini, L; Yates, Vance J.

doi:10.2307/1588235

Cited by 1 publication

(2 citation statements)

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“…As already described by MArcia1 and YATES (11) and CHOMIAK et al (2), CELO virus multiplies only poorly in chicken embryo fibroblasts as compared to chicken kidney cells. Experiments with a CELO virus strain isolated in our laboratory confirmed these observations.…”

Section: Discussionsupporting

confidence: 55%

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Cell cycle-dependent multiplication of avian adenoviruses in chicken embryo fibroblasts

Kraft

Tischer

1978

Archives of Virology

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Propagation of CELO virus employing confluent monolayers of chicken enbryo fibroblasts (CEF) yielded virus titers one to two logs lower than those from confluent chicken kidney (CK) cells. An enhancement of virus production in CEF as measured by plaque formation was obtainedby infectng cultures in the growing non confluent state. Measurements of 3H-thymidine incorporation revealed a positive correlation between the DNA synthesis of CEF cultures at the time of inoculation and the amount of progeny virus, whereas in the CK-CELO-system no such relation was observed. Requirement of replicative fibroblasts for CELO multiplication was also demonstrated by comparison of virus replication in synchronized stationary and serum stimulated CEF cells. In stationary CEF cells arrested in the G1 phase of the cell replication cycle by serum deprivation and infected withe CELO virs, no cytopathic effect could be observed, and only very low amounts of virus were produced. But 24 hours after release of these cells for growth by serum stimulation a logarithmic rate of virus multiplication and a complete CPE occurred. Infection of synchronized CEF cultures at different stages of the cell cycle revealed that CELO multiplication was correlated with the S phase of the infected cell. In synchronized CELO infected CEF cultures viral DNA synthesis started 12 to 14 hours after growth stimulation when cells were near the end of the S phase. In contrast, no viral DNA synthesis could be measured in growth arrested CELO infected CEF cells, when cellular DNA synthesis was low. Therefore not only production of infectious virus but also viral DNA synthesis is correlated with events during the S phase of the infected CEF cell.

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Section: Discussionsupporting

confidence: 55%

“…CELO virus replicates in avian k i d n e y and liver cells equally well, but infection of chick embryo fibroblasts yielded virus titers one to two logs lower t h a n those from kidney and liver cells (2,11,12). The present, studies were performed to elucidate the reason for the low multiplication rate in chick fibroblasts.…”

Section: Introductionmentioning

confidence: 99%

Cell cycle-dependent multiplication of avian adenoviruses in chicken embryo fibroblasts

Kraft

Tischer

1978

Archives of Virology

View full text Add to dashboard Cite

show abstract

Growth of Chicken-Embryo-Lethal-Orphan (CELO) Virus in Chicken Embryo Neuroglial and Chicken Embryo Fibroblastic Cell Cultures

Cited by 1 publication

References 5 publications

Cell cycle-dependent multiplication of avian adenoviruses in chicken embryo fibroblasts

Cell cycle-dependent multiplication of avian adenoviruses in chicken embryo fibroblasts

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