Growth of Candida famata and Trichosporon cutaneum on uric acid as the sole source of carbon and energy, a hitherto unknown property of yeasts

Middelhoven, W.J.; Brink, Johan; Veenhuis, Marten

doi:10.1007/bf00399316

Cited by 20 publications

(14 citation statements)

References 7 publications

(3 reference statements)

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“…The growth medium supplemented with five antibacterial antibiotics was as described earlier (Middelhoven et al, 1983). However, the concentration of KH2PO4 was 20 g-1 -a instead of 1 g. 1-1 to increase the buffering capacity.…”

Section: Enrichment Culturesmentioning

confidence: 99%

“…These phenomena have been considered from a taxonomic point of view (Hazeu et al, 1972;Bos and De Bruyn, 1973). Recently, utilization by some yeasts of uric acid (Middelhoven et al, 1983) and adenine (Middelhoven et al, 1984) as sole carbon source was demonstrated. All of these yeast strains were able to utilize some primary n-alkylamines, e.g.…”

Section: Introductionmentioning

confidence: 97%

“…Growth of yeasts at the expense of alkylamines, uric acid or hydrocarbons is accompanied with the development ofmicrobodies, cell organelles that display the function of peroxisomes, of glyoxisomes or both (Middelhoven et al, 1983;Veenhuis and Harder, 1985;. Peroxisomes are also abundant in methanol-grown yeast cells (Van Dijken et al, 1975).…”

Section: Introductionmentioning

confidence: 98%

“…Candidafamata TOX-2, Trichosporon cutaneum TOU-17 (Middelhoven et al, 1983) and Tr. adeninovorans TOA-3 (Middelhoven et al, 1984) showed a relatively high optimum temperature and tolerated high osmotic pressure.…”

Section: Introductionmentioning

confidence: 99%

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Yeast species utilizing uric acid, adenine,n-alkylamines or diamines as sole source of carbon and energy

Middelhoven¹,

Kievit²,

Biesbroek³

1985

Antonie van Leeuwenhoek

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Yeast strains utilizing uric acid, adenine, monoamines or diamines as sole source of carbon and energy were isolated from several soil samples by the enrichment culture method. The most common species was Trichosporon cutaneum. Strains of Candida catenulata, C. famata, C. parapsilosis, C. rugosa, Cryptococcus laurentii, Stephanoascus ciferrii and Tr. adeninovorans were also isolated. All strains utilizing uric acid as sole carbon source utilized some primary n-alkyl-l-amines, hydroxyamines or diamines as well. The ascomycetous yeast strains showing these characteristics all belonged to species known to assimilate hydrocarbons. Type strains of hydrocarbon-positive yeast species which were not found in the enrichment cultures generally assimilated putrescine, some type strains also butylamine or pentylamine, but none assimilated uric acid. Methanol-positive species were not isolated. Type strains of methanol-positive and of hydrocarbon-negative species did not assimilate uric acid, butylamine or putrescine. Assimilation of putrescine as sole source of carbon and energy may be a valuable diagnostic criterion in yeast taxonomy.

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Section: Enrichment Culturesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Yeast species utilizing uric acid, adenine,n-alkylamines or diamines as sole source of carbon and energy

Middelhoven¹,

Kievit²,

Biesbroek³

1985

Antonie van Leeuwenhoek

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“…Uric acid is one of these nonconventional substrates (Middelhoven et al 1983). Like other aerobic microorganisms, ascomycetous and a basidiomycetous yeast species degrade uric acid by oxidation to allantoin that, in three successive hydrolytic steps, is converted into 1 mol glyoxylate and 2 mol urea (Middelhoven et al 1983).…”

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confidence: 99%

The yeast flora of some decaying mushrooms on trunks of living trees

Middelhoven

2004

Folia Microbiol

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ABSTRACT. Several ascomycetous and basidiomycetous yeasts were isolated from rotten mushrooms on the trunks of beech and tamarisk trees. One strain, identified as the novel species Cryptococcus allantoinivorans, assimilated allantoin as the sole carbon source. Phylogenetically it belongs to the C. laurentii complex, Papiliotrema bandonii being the closest relative. Some ascomycetous strains could not be distinguished from Pichia guillermondii, but deviated considerably in rDNA sequences. In addition to these species, both decaying mushrooms were inhabited by more common species, viz. Candida albicans, C. saitoana, Rhodotorula mucilaginosa, Trichosporon asahii, T. multisporum and T. porosum. The basidiomycetous yeasts, except R. mucilaginosa, assimilated some polysaccharides of plant origin.Several yeast species assimilate a great variety of carbon sources in addition to those used for yeast identification and taxonomy (Middelhoven et al. 1985(Middelhoven et al. , 1991(Middelhoven et al. , 2001(Middelhoven et al. , 2004aMiddelhoven and Kurtzman 2003). Uric acid is one of these nonconventional substrates (Middelhoven et al. 1983). Like other aerobic microorganisms, ascomycetous and a basidiomycetous yeast species degrade uric acid by oxidation to allantoin that, in three successive hydrolytic steps, is converted into 1 mol glyoxylate and 2 mol urea (Middelhoven et al. 1983). Surprisingly, most yeast species assimilating uric acid as the sole carbon source failed to grow at the expense of allantoin, in spite of equal energetic value. Middelhoven et al. (1985) demonstrated that of 14 ascomycetous and basidiomycetous yeast species, growing on uric acid as the sole carbon source, only Stephanoascus ciferrii M.TH. SMITH et al. assimilated allantoin. It is wide-spread in nature and is a source of nitrogen for many yeast species. Nevertheless, several attempts to isolate yeasts from soil by enrichment culture on allantoin as the sole carbon source failed. In the present study such yeasts were looked for in another habitat, viz. decaying mushrooms. It has been known for a long time that mushrooms contain allantoin (Brunel 1931(Brunel , 1936. Mushrooms also contain chitin as a constituent of the fungal cell wall. Assimilation of this polysaccharide by yeasts has never been reported. Attempts were made to isolate such yeasts from decaying mushrooms.The present study was not restricted to isolation of yeasts with characteristic biochemical abilities. In addition, several yeast species present in decaying mushrooms were identified as representatives of wellknown species. However, some very rare species and some strains physiologically similar to Pichia guillermondii but belonging to undescribed species were also isolated. The basidiomycetous yeasts were tested for growth on several polysaccharides of plant origin. MATERIALS AND METHODSIsolation of the strains. The rotting mushrooms sampled were Hericium erinaceus (BULL.:FR.)PERS. on the trunk of a beech tree (Fagus sylvatica L.) in Wageningen (The Netherlands) September 2000,...

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The yeast flora of ensiled whole‐crop maize

Middelhoven¹,

Franzen²

1986

J Sci Food Agric

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The yeast flora in samples of 13 different whole‐crop maize silages was shown to be predominated by Candida krusei, C. lambica, Saccharomyces dairensis, S. exiguus, C. holmii or C. milleri. All these strains fermented glucose but, except for the latter three species, not sucrose, α‐trehalose or raffinose. Under conditions resembling those in silage, i.e. at pH 4.0 in the presence of a complex nitrogen source, all strains assimilated lactic and acetic acids. This result suggests that the predominant yeasts in silage should not be distinguished into lactate utilisers and lactate non‐utilisers. All strains, except those of Saccharomyces dairensis, tolerated acetic acid (5 g litre−1) and grew at pH 4.0 in a mineral salts medium containing lactic acid (10 g litre−1), acetic acid (5 g litre−1) and yeast extract (1 g litre−1). Growth in this medium was completely inhibited by the cationic detergent Arquad C33W (20 mg litre−1), propionic acid (5 g litre−1), benzoic acid (5 g litre−1) or salicylic acid (10 g litre−1).

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Growth of Candida famata and Trichosporon cutaneum on uric acid as the sole source of carbon and energy, a hitherto unknown property of yeasts

Cited by 20 publications

References 7 publications

Yeast species utilizing uric acid, adenine,n-alkylamines or diamines as sole source of carbon and energy

Yeast species utilizing uric acid, adenine,n-alkylamines or diamines as sole source of carbon and energy

The yeast flora of some decaying mushrooms on trunks of living trees

The yeast flora of ensiled whole‐crop maize

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