Growth of bifidobacteria in a fermented wheat germ medium

Lactobacillus apis sp. nov., from the stomach of honeybees (Apis mellifera), having an in vitro inhibitory effect on the causative agents of American and European foulbrood

“…Captured honeybees from a hive were transported to the laboratory and then decapitated and the stomach contents were serially diluted in tubes containing sterile anaerobic MRS broth (Oxoid). Aliquots of 0.1 ml were placed on fermented wheatgerm medium (FWGM) (Dubná et al , 2010) which contained free amino acids and peptides that improve the growth of lactobacilli. Samples were cultivated at 37 °C under anaerobic conditions (Oxoid anaerobic jars) for 48 h. Bacterial colonies were picked, transferred to tubes containing anaerobic FWGM and cultivated at 37 °C for 16 h.…”