Growth of a Highly Porous Coordination Polymer on a Macroporous Polymer Monolith Support for Enhanced Immobilized Metal Ion Affinity Chromatographic Enrichment of Phosphopeptides

Saeed, Adeela; Maya, Fernando; Xiao, Dianne J.; Najam‐ul‐Haq, Muhammad; Švec, František; Britt, David K.

doi:10.1002/adfm.201400116

Cited by 62 publications

(52 citation statements)

References 36 publications

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“…The residue at 600 °C is α-Fe 2 O 3 , as confirmed by powder Xray diffraction. The presence of iron in the capillary column format is detected by energy dispersive X-ray spectroscopy 21 . Figure 3 shows an example of a real sample application of the developed MOPH material for the enrichment of phosphopeptides from a digestion of nonfat milk.…”

Section: Representative Resultsmentioning

confidence: 99%

Preparation of Highly Porous Coordination Polymer Coatings on Macroporous Polymer Monoliths for Enhanced Enrichment of Phosphopeptides

Lamprou

Wang

Saeed

et al. 2015

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We describe a protocol for the preparation of hybrid materials based on highly porous coordination polymer coatings on the internal surface of macroporous polymer monoliths. The developed approach is based on the preparation of a macroporous polymer containing carboxylic acid functional groups and the subsequent step-by-step solution-based controlled growth of a layer of a porous coordination polymer on the surface of the pores of the polymer monolith. The prepared metal-organic polymer hybrid has a high specific micropore surface area. The amount of iron(III) sites is enhanced through metal-organic coordination on the surface of the pores of the functional polymer support. The increase of metal sites is related to the number of iterations of the coating process.The developed preparation scheme is easily adapted to a capillary column format. The functional porous polymer is prepared as a self-contained single-block porous monolith within the capillary, yielding a flow-through separation device with excellent flow permeability and modest backpressure. The metal-organic polymer hybrid column showed excellent performance for the enrichment of phosphopeptides from digested proteins and their subsequent detection using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. The presented experimental protocol is highly versatile, and can be easily implemented to different organic polymer supports and coatings with a plethora of porous coordination polymers and metal-organic frameworks for multiple purification and/or separation applications. Video LinkThe video component of this article can be found at

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Section: Representative Resultsmentioning

confidence: 99%

Preparation of Highly Porous Coordination Polymer Coatings on Macroporous Polymer Monoliths for Enhanced Enrichment of Phosphopeptides

Lamprou

Wang

Saeed

et al. 2015

JoVE

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“…This information is available free of charge via the Internet at http://pubs.acs.org/. 6 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 …”

Section: Supporting Information Availablementioning

confidence: 98%

Automatic In-Syringe Dispersive Microsolid Phase Extraction Using Magnetic Metal–Organic Frameworks

et al. 2015

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A novel automatic strategy for the use of micro- and nanomaterials as sorbents for dispersive microsolid phase extraction (D-μ-SPE) based on the lab-in-syringe concept is reported. Using the developed technique, the implementation of magnetic metal-organic framework (MOF) materials for automatic solid-phase extraction has been achieved for the first time. A hybrid material based on submicrometric MOF crystals containing Fe3O4 nanoparticles was prepared and retained in the surface of a miniature magnetic bar. The magnetic bar was placed inside the syringe of an automatic bidirectional syringe pump, enabling dispersion and subsequent magnetic retrieval of the MOF hybrid material by automatic activation/deactivation of magnetic stirring. Using malachite green (MG) as a model adsorption analyte, a limit of detection of 0.012 mg/L and a linear working range of 0.04-2 mg/L were obtained for a sample volume equal to the syringe volume (5 mL). MG preconcentration was linear up to a volume of 40 mL, obtaining an enrichment factor of 120. The analysis throughput is 18 h(-1), and up to 3000 extractions/g of material can be performed. Recoveries ranging between 95 and 107% were obtained for the analysis of MG in different types of water and trout fish samples. The developed automatic D-μ-SPE technique is a safe alternative for the use of small-sized materials for sample preparation and is readily implementable to other magnetic materials independent of their size and shape and can be easily hyphenated to the majority of detectors and separation techniques.

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“…In this work, methanol was used as mobile phase and corresponding value of dynamic 30 viscosity was 0.580×10 -3 kg/(m×s) at 25 ℃.…”

Section: Calculationsmentioning

confidence: 99%

“…1 In recent times, MOFs have 25 attracted considerable attention in various fields, including gas storage, 2 energy storage, 3 catalysis, 4 drug delivery, 5 sensing, 6 and separation, 7 due to their diverse structures and fascinating properties, such as exceptionally large surface area, highly uniform pores, and tunable pore geometry 8 . These unique 30 properties and wide structural choices also make MOFs attractive candidates for stationary phases in solid-phase extraction 9 and chromatography, 10,11 specifically for packed columns and coated capillary columns in high resolution gas chromatography (GC). 12 Recently, various MOFs (particles/powders of MIL-101, MIL-53, 35 MIL-100, HKUST-1, MOF-5 and MIL-47) have been ground or directly packed into columns as the stationary phase for high performance liquid chromatography (HPLC).…”

Section: Introductionmentioning

confidence: 99%

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In situ synthesis of metal–organic frameworks in a porous polymer monolith as the stationary phase for capillary liquid chromatography

Yang

Zhao

et al. 2015

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In this study, HKUST-1 was synthesized in situ on the porous polymer monolith as the stationary phase for capillary liquid chromatography (cLC). The unique carboxyl functionalized poly(methacrylic acid-co-ethylene dimethacrylate) (poly(MAA-co-EDMA)) monolith was used as a support to directly grow HKUST-1 by a controlled layer-by-layer self-assembly strategy. X-ray diffraction, scanning electron microscopy, energy dispersive X-ray spectrometry, and Fourier transform infrared spectroscopy of the resulting HKUST-1-poly(MAA-co-EDMA) monoliths indicated that HKUST-1 was successfully grafted onto the pore surface of the poly(MAA-co-EDMA) monolith. The column performance of HKUST-1-poly(MAA-co-EDMA) monoliths for the separation of various small molecules, such as benzenediols, xylenes, ethylbenzenes, and styrenes, was evaluated. The chromatographic performance was found to improve with increasing HKUST-1 density, and the column efficiencies and resolutions of HKUST-1-poly(MAA-co-EDMA) monoliths were 18 320-19 890 plates m(-1) and 1.62-6.42, respectively, for benzenediols. The HKUST-1-poly(MAA-co-EDMA) monolith displayed enhanced resolution for the separation of positional isomers when compared to the traditional C18 and HKUST-1 incorporated polymer monoliths. Hydrophobic, π-π, and hydrogen bonding interactions within the HKUST-1-poly(MAA-co-EDMA) monolith were observed in the separation of small molecules. The results showed that the HKUST-1-poly(MAA-co-EDMA) monoliths are promising stationary phases for cLC.

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Growth of a Highly Porous Coordination Polymer on a Macroporous Polymer Monolith Support for Enhanced Immobilized Metal Ion Affinity Chromatographic Enrichment of Phosphopeptides

Cited by 62 publications

References 36 publications

Preparation of Highly Porous Coordination Polymer Coatings on Macroporous Polymer Monoliths for Enhanced Enrichment of Phosphopeptides

Preparation of Highly Porous Coordination Polymer Coatings on Macroporous Polymer Monoliths for Enhanced Enrichment of Phosphopeptides

Automatic In-Syringe Dispersive Microsolid Phase Extraction Using Magnetic Metal–Organic Frameworks

In situ synthesis of metal–organic frameworks in a porous polymer monolith as the stationary phase for capillary liquid chromatography

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