Growth, nutrient uptake and chemical composition of Chlorella sp. and Nannochloropsis oculata under nitrogen starvation

Paes, C. R. P. S.; Faria, Gabrielle Rodrigues de; Tinoco, Natália A. B.; Castro, Dominique J.F.A; Barbarino, Elisabete; Lourenço, Simone de Queiroz Chaves

doi:10.3856/vol44-issue2-fulltext-9

Cited by 76 publications

(40 citation statements)

References 81 publications

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“…In this phase, the lipid content is lower than in the other phases. During the exponential phase, microalgae tend to use nutrients for the process of division and protein synthesis [12]. Proteins during this phase are needed to form cell structures.…”

Section: Lipid Content Chaetoceros Muellerimentioning

confidence: 99%

Effect of Salinity Difference on Lipid Content from Chaetoceros muelleri on Continuous Reactors

2019

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Chaetoceros muelleri is a microalgae class of Bacillariophyta (diatom) which is generally only used as feeds for fishes and shellfish larvae. Nevertheless, the biochemical content of this species is quite high and has the potential to be developed. This research aims to explain the effect of different salinity on the growth and lipid content of Chaetoceros muelleri cultured in a continuous photobioreactor. This research was carried out in August 2018 - February 2019. The research was conducted at the Laboratory of Marine Microbiology and the Laboratory of Bioprocess and Bioprospection of Natural Materials, Faculty of Fisheries and Marine Sciences, Padjadjaran University. The samples of Chaetoceros muelleri isolates were obtained from the Jepara Brackish Water Aquaculture Center. The methods used for the study was a ‘Completely Randomized Design’ (CRD) with four treatments. The salinity used is 15, 25, 35 and 45 ppt. The main parameters observed were growth and lipid content, while the supporting parameters were temperature, and pH. The results of this study showed that the highest lipid content was a salinity treatment of 35 ppt with a value of 25.37% of total dry weight obtained at the end of the culture. Based on growth, the highest density occurred in 25 ppt salinity with a maximum density of 3.80 ± 0.49 x 106 cells. ml-1 and maximum growth rate of 0.36 ± 0.008 div. day-1

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Section: Lipid Content Chaetoceros Muellerimentioning

confidence: 99%

Effect of Salinity Difference on Lipid Content from Chaetoceros muelleri on Continuous Reactors

2019

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“…This result was even higher than the previous findings where the maximum cell density of Chlorella sp. was around 6.5 x10 6 cell mL -1 [11]. This high cell density of algae reveals that Chlorella sp.…”

Section: Growth Studymentioning

confidence: 81%

“…The initial Chlorella sp. concentration varied between 0 -70 x10 4 cell mL -1 [11,12]. The flasks were aerated to provide CO 2 and for mixing via an air pump (flowrate of 400 mL/min with 2% CO 2 ) [13].…”

Section: Methodsmentioning

confidence: 99%

Removal of Inorganic Nutrient and Organic Carbon from Wastewater of Binh Dien Market Using Green Alga Chlorella sp

Bùi¹,

Duong²,

Luong³

2016

GeoScience Engineering

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Traditional markets play a major role in socio-economics and constitutes a significant aspect of Vietnamese culture. However, wastewater streams discharged from the markets are generally characterized by a lot of inorganic nutrients and organic substances originated from fresh food processing units. They could lead to serious water contamination if discharged without proper treatment. This study applied microalgae Chlorella sp. for eliminating inorganic nutrients (NO3−-N, NH4+-N and PO43−-P) and organic carbon (Chemical oxygen demand-COD) from wastewater of the Binh Dien market. The removal efficiencies reached for NH4+-N > 86%, for NO3−-N > 72%, and for PO43−-P > 69%, respectively, at algal density of 49 × 104 cell mL−1, and for COD > 96% at algal density of 35 × 104 cell mL−1 after five cultivating days. The effluence satisfied the Vietnamese standard, column B, of the National technical regulation on industrial wastewater (QCVN 40:2011/BTNMT). The results demonstrated that the culture system composed of green algal Chlorella sp. could be a potential candidate for the removal of nutrients and organic carbon by a wastewater treatment process from the Binh Dien market.

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“…Nitrogen is mainly contained in proteins and, to a lesser extent, in glycolipids from the cell walls and [40,41]. Cultivation media MC1 (for A. maxima) and MC4 (for N. gaditana and C. vulgaris) had a nitrate concentration of 134 mg/L.…”

Section: Biomass Compositionmentioning

confidence: 99%

“…The CO2 emitted after their use corresponds to that fixed from atmosphere by microalgae, so that no net CO2 emission is produced. Although the number of different algae and cyanobacteria species in nature is unknown, conservative estimations give values above 70,000 of which more than 40,000 have been published [1]. Because of this enormous number of species, in this work we have selected four different microorganisms of representative genera.…”

Section: Introductionmentioning

confidence: 99%

Effect of Cultivation Variables on Biomass Composition and Growth of Microalgae and Cyanobacteria

Sánchez-Bayo¹,

Morales²,

Rodríguez³

et al. 2019

Preprint

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The purpose of this work is to define optimal growth conditions for batch culture of the cyanobacterium Arthrospira maxima and the microalgae Chlorella vulgaris, Isochrysis galbana and Nannochloropsis gaditana. Thus, we study the effect of three variables on algae growth: i.e., inoculum:culture medium ratio, light:darkness photoperiod and type of culture medium, including both synthetic media and wastewaters. The results showed that the initial inoculum volume did not affect the amount of biomass at the end of the growth (14 days), whereas an excess (18 h) or defect (6 h) in the number of hours of light is determinant for its development. The contribution of nutrients from different culture media modified the growth of the different species. A. maxima was favoured in seawater enriched with Guillard's F/2 as well as C. vulgaris and N. gaditana but in fresh water medium. I. galbana had the greatest growth in the marine environment enriched with Walne’s media. Nitrate was the limiting growth reagent at the end of the exponential phase of growth for C. vulgaris and N. gaditana, while iron was for A. maxima and I. galbana. All species demonstrated their capability to grow in effluents from a wastewater treatment plant and they efficiently consume nitrogen, especially the three microalgae species.

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Growth, nutrient uptake and chemical composition of Chlorella sp. and Nannochloropsis oculata under nitrogen starvation

Cited by 76 publications

References 81 publications

Effect of Salinity Difference on Lipid Content from Chaetoceros muelleri on Continuous Reactors

Effect of Salinity Difference on Lipid Content from Chaetoceros muelleri on Continuous Reactors

Removal of Inorganic Nutrient and Organic Carbon from Wastewater of Binh Dien Market Using Green Alga Chlorella sp

Effect of Cultivation Variables on Biomass Composition and Growth of Microalgae and Cyanobacteria

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