Growth modeling to control ( in vitro )  Fusarium verticillioides  and  Rhizopus stolonifer  with thymol and carvacrol

Ochoa‐Velasco, C.E.; Navarro-Cruz, Addí Rhode; Vera-López, Obdulia; Palou, Enrique; Avila-Sosa, Raúl

doi:10.1016/j.ram.2016.11.010

Cited by 27 publications

(27 citation statements)

References 12 publications

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“…The MIC is the lowest concentration of the extract capable of stopping the growth of a microorganism (Tripathi, 2013). In this study, the MIC was 7.81 for both fungal extracts for most of the bacteria tested, except for E. coli (31.25) and E. faecalis (15.62) for the Fusarium 23952 extract, similarly to the results of other studies on MIC determination in antimicrobial activity (Nisa, 2015;Ochoa-Velasco et al, 2018;Naime-Shamel et al, 2019).…”

Section: Discussionsupporting

confidence: 86%

Chromatography and bioautography of endophytic fungi extracts of Uncaria tomentosa (Willd.) DC with antibacterial activity

Gonã‡Alves¹,

ARAÃšJO²,

Brasil³

et al. 2019

J. Med. Plants Res.

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Endophytic fungi of different species have already proved their efficacy against a range of pathogenic bacteria. However, the separation of the compounds contained in these extracts that actually have an effective activity is not a constant, but it is essential for the scientific knowledge to formulate a new medication. Thus, this study aimed to separate compounds from two endophytic fungi extracts of Uncaria tomentosa with positive results against Gram-positive and Gram-negative bacteria. The separation was carried out by thin-layer chromatography and bioautography. For this, it was necessary to produce fungal extracts from the endophytes Colletotrichum (23916) and Fusarium (23952), which were separated from the liquid state by filtration. The remaining filtrate was divided into ethyl acetate and then concentrated in a rotary evaporator at 40°C. Antimicrobial activity was evaluated and the Minimum Inhibitory Concentration (MIC) was determined with the following test microorganisms: Escherichia coli, Enterecoccus faecalis, Klebsiella pneumoniae and Staphylococcus aureus. Subsequently, it was carried out the thin layer chromatography (TLC) of the extracts using hexane, ethyl acetate/hexane, ethyl acetate and acetate/methanol. The reading was performed in 312 nm UV light, and right after the chromatography, the bioautography was carried out. The following Rf values were obtained: 0.30 for K. pneumoniae and 0.35 for E. coli, in ethyl acetate, for the extract 23952, and 0.81 for K. pneumoniae and 0.41 for S. aureus, in styrene acetate, for the extract 23916, confirming different compounds by bioautography. Therefore, it was concluded that several compounds present in the extracts tested have antibacterial activity.

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Section: Discussionsupporting

confidence: 86%

Chromatography and bioautography of endophytic fungi extracts of Uncaria tomentosa (Willd.) DC with antibacterial activity

Gonã‡Alves¹,

ARAÃšJO²,

Brasil³

et al. 2019

J. Med. Plants Res.

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“…Carvacrol and eugenol are the principal aromatic active components of oregano oil and clove oil, respectively, and have a wide spectrum antimicroorganism activity, preventing food from fungal spoilage and foodborne bacteria (Nostro et al, ). In vitro studies have showed that carvacrol and eugenol could inhibit the growth of Botrytis cinerea , Phytophthora cactorum , Cryphonectria parasitica , and Fusarium circinatum (Ochoavelasco, Navarrocruz, Veralópez, Palou, & Avilasosa, ; Santamarina et al, ). Carvacrol and eugenol exhibited high antifungal activity against R. stolonifer in vitro in the present study.…”

Section: Discussionmentioning

confidence: 99%

Carvacrol and eugenol inhibit postharvest soft rot disease by enhancing defense response in peaches during storage

Zhou

Wei

Peng

et al. 2019

J Food Process Preserv

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Peaches are in high demands because of their nutritional value and delicious flavor, but often become unmarketable due to the fungus infection. In this study, carvacrol and eugenol were used to preserve peaches. Rhizopus stolonifer growth was completely inhibited by carvacrol at 32 µl/Lair and eugenol at 64 µl/Lair. Carvacrol at 0.5 µl/Lair and eugenol at 1 µl/Lair significantly reduced the incidence and severity of soft rot decay in artificially inoculated peaches. Also, carvacrol and eugenol significantly reduced the natural incidence of soft rot disease and maintained high sensory acceptance and quality of peaches during storage. The activities of defense-related enzymes were enhanced by carvacrol and eugenol fumigation, and the contents of phenolics, flavonoids, lignin, and hydroxyproline-rich glycoprotein in these two oils treated fruit, were much higher than untreated control during storage. These observations indicted that carvacrol and eugenol could preserve peach fruit during postharvest storage. Practical applicationsCarvacrol and eugenol showed great potential application in the preservation of peach fruit during postharvest storage, exhibiting high antifungal activity against Rhizopus stolonifer and enhancing defense response to soft rot disease.

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“…Kordali et al [27] presented complete inhibition of fungi from the genera of Alternaria, Botrytis, Fusarium, Pythium, Monilia, Phytophtora, Sclerotinia, and Verticillium. In the research of Ochoa-Velascoa et al [58], these compounds were effective at very low concentrations against F. verticillioides and Rhizopus stolonifer. Carvacrol and thymol may cause alterations in hyphal morphology reducing their diameter and causing lysis of hyphal walls [59].…”

Section: Discussionmentioning

confidence: 96%

The Inhibitory Potential of Selected Essential Oils on Fusarium spp. Growth and Mycotoxins Biosynthesis in Maize Seeds

et al. 2019

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Owing to their rich chemical composition, essential oils (EOs) have many interesting properties, including antimicrobial activities. The presence of Fusarium and their secondary metabolites, mycotoxins, in cereal crops is a serious problem in agriculture, which consequently affects food quality. The aim of the present study was to investigate the effects of selected EOs on the growth of Fusarium graminearum and F. culmorum and the biosynthesis of mycotoxins in maize seeds. Chromatographic analysis of ergosterol as a fungal growth indicator showed a significant inhibition of Fusarium growth (83.24–99.99%) compared to the control samples, which as a consequence resulted in a reduction in mycotoxin concentrations. The addition of cinnamon, palmarosa, orange, and spearmint EOs was shown to be the most effective in reducing zearalenone concentration (99.10–99.92%). Deoxynivalenol analysis confirmed a very high reduction of this compound at the application all tested EOs (90.69–100%). The obtained results indicated that EOs have a great potential to inhibit growth of Fusarium fungi as well as reduce the concentration of mycotoxins in maize seed.

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Growth modeling to control ( in vitro ) Fusarium verticillioides and Rhizopus stolonifer with thymol and carvacrol

Cited by 27 publications

References 12 publications

Chromatography and bioautography of endophytic fungi extracts of Uncaria tomentosa (Willd.) DC with antibacterial activity

Chromatography and bioautography of endophytic fungi extracts of Uncaria tomentosa (Willd.) DC with antibacterial activity

Carvacrol and eugenol inhibit postharvest soft rot disease by enhancing defense response in peaches during storage

The Inhibitory Potential of Selected Essential Oils on Fusarium spp. Growth and Mycotoxins Biosynthesis in Maize Seeds

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