Growth, microtuber production and physiological metabolism in virus-free and virus-infected potato in vitro plantlets grown under NaCl-induced salt stress

Li, Jingwei; Chen, Haiyan; Li, Jiao; Zhang, Zhibo; Blystad, Dag‐Ragnar

doi:10.1007/s10658-018-1485-9

Cited by 19 publications

(7 citation statements)

References 77 publications

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“…Results obtained in the present study clearly demonstrate that PVS, PVA, and PVM in single or mixed infection negatively affect microtuber production of in vitro cultures of “Dunluce,” “Tahi,” and “V500.” This finding is consistent with results reported by Li et al (2013 , 2018b ) and Zhang et al (2019) . Li et al (2013) and Zhang et al (2019) also noted that the co-infections of PLRV + PVY and PLRV + PVS and the triple infection of PVY and PVX + PVS could lead to negative effects on vegetative growth of potato in vitro .…”

Section: Discussionsupporting

confidence: 94%

Eradication of Potato Virus S, Potato Virus A, and Potato Virus M From Infected in vitro-Grown Potato Shoots Using in vitro Therapies

et al. 2022

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Certain viruses dramatically affect yield and quality of potatoes and have proved difficult to eradicate with current approaches. Here, we describe a reliable and efficient virus eradication method that is high throughput and more efficacious at producing virus-free potato plants than current reported methods. Thermotherapy, chemotherapy, and cryotherapy treatments were tested alone and in combination for ability to eradicate single and mixed Potato virus S (PVS), Potato virus A (PVA), and Potato virus M (PVM) infections from three potato cultivars. Chemotherapy treatments were undertaken on in vitro shoot segments for four weeks in culture medium supplemented with 100 mg L−1 ribavirin. Thermotherapy on in vitro shoot segments was applied for two weeks at 40°C (day) and 28°C (night) with a 16 h photoperiod. Plant vitrification solution 2 (PVS2) and cryotherapy treatments included a shoot tip preculture followed by exposure to PVS2 either without or with liquid nitrogen (LN, cryotherapy) treatment. The virus status of control and recovered plants following therapies was assessed in post-regeneration culture after 3 months and then retested in plants after they had been growing in a greenhouse for a further 3 months. Microtuber production was investigated using in vitro virus-free and virus-infected segments. We found that thermotherapy and cryotherapy (60 min PVS2 + LN) used alone were not effective in virus eradication, while chemotherapy was better but with variable efficacy (20–100%). The most effective result (70–100% virus eradication) was obtained by combining chemotherapy with cryotherapy, or by consecutive chemotherapy, combined chemotherapy and thermotherapy, then cryotherapy treatments irrespective of cultivar. Regrowth following the two best virus eradication treatments was similar ranging from 8.6 to 29% across the three cultivars. The importance of virus removal on yield was reflected in “Dunluce” free of PVS having higher numbers of microtubers and in “V500’ free of PVS and PVA having a greater proportion of microtubers > 5 mm. Our improved procedure has potential for producing virus-free planting material for the potato industry. It could also underpin the global exchange of virus-free germplasm for conservation and breeding programs.

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Section: Discussionsupporting

confidence: 94%

Eradication of Potato Virus S, Potato Virus A, and Potato Virus M From Infected in vitro-Grown Potato Shoots Using in vitro Therapies

et al. 2022

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“…Based on this fact, Wang et al (2009) and Wang and Valkonen (2009) defined cryotherapy as a technique that uses extreme low temperature of LN to eradicate plant pathogens from diseased tissue (Figure 1). Further studies have found that in addition to disease eradication, shoot tip cryopreservation has a double‐edged sword effect on pathogen eradication and long‐term preservation (Li, Chen, et al, 2018; Wang et al, 2022; Wang, Yang, et al, 2018). This subject is not included in this review, and detailed information can be found in the review of Zhao et al (2019).…”

Section: Establishment and Development Of Shoot Tip Cryotherapymentioning

confidence: 99%

“…Li, Chen, et al (2018) compared growth, microtuber production and physiological metabolism between the cryoderived virus‐free plants and the virus‐infected plants of potato grown under NaCl‐induced salt stress in in vitro conditions. Cryoderived virus‐free plantlets (healthy) and PLRV‐ or PVY O ‐ or PVS‐infected plantlets (either single or co‐infection) were included in the study.…”

Section: Assessment Of Pathogen‐free Plants Derived From Shoot Tip Cr...mentioning

confidence: 99%

Shoot tip cryotherapy for plant pathogen eradication

Bettoni

Zhang

et al. 2022

Plant Pathology

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Diseases caused by plant pathogens such as viruses, viroids and phytoplasmas cause huge economic losses of agricultural production and limit the safe movement of plant materials across borders. The use of pathogen-free planting materials provides a strategy for efficient management of these diseases and facilitates the global exchange of genetic resources. Shoot tip cryotherapy is a novel biotechnology method that uses cryogenic procedures to eradicate plant pathogens from the diseased plants.Combining thermotherapy or chemotherapy with shoot tip cryotherapy has further enhanced pathogen eradication efficiency. This review provides updated and comprehensive information on shoot tip cryotherapy and the combination of thermotherapy or chemotherapy with shoot tip cryotherapy for pathogen eradication. Prospects are proposed for future studies.

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“…FCM did not detect any variations in ploidy levels in Chinese jujube plants ( Ziziphus jujuba ), free of Jujube witches’ broom phytoplasma [ 107 ], and artichoke plants ( Cynara scolymus ), free of artichoke latent virus, produced by shoot tip cryotherapy [ 108 ]. The SSR and AFLP did not detect any polymorphic bands in cryo-derived potato plants ( Solanum tuberosum ) free of potato leafroll virus, potato virus S, and potato virus Y [ 109 ].…”

Section: Assessments Of Epigenetic and Genetic Integritymentioning

confidence: 99%

Epigenetic and Genetic Integrity, Metabolic Stability, and Field Performance of Cryopreserved Plants

Shukla

Ren

et al. 2021

Plants

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Cryopreservation is considered an ideal strategy for the long-term preservation of plant genetic resources. Significant progress was achieved over the past several decades, resulting in the successful cryopreservation of the genetic resources of diverse plant species. Cryopreservation procedures often employ in vitro culture techniques and require the precise control of several steps, such as the excision of explants, preculture, osmo- and cryoprotection, dehydration, freeze-thaw cycle, unloading, and post-culture for the recovery of plants. These processes create a stressful environment and cause reactive oxygen species (ROS)-induced oxidative stress, which is detrimental to the growth and regeneration of tissues and plants from cryopreserved tissues. ROS-induced oxidative stresses were documented to induce (epi)genetic and somatic variations. Therefore, the development of true-to-type regenerants of the source germplasm is of primary concern in the application of plant cryopreservation technology. The present article provides a comprehensive assessment of epigenetic and genetic integrity, metabolic stability, and field performance of cryopreserved plants developed in the past decade. Potential areas and the directions of future research in plant cryopreservation are also proposed.

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Growth, microtuber production and physiological metabolism in virus-free and virus-infected potato in vitro plantlets grown under NaCl-induced salt stress

Cited by 19 publications

References 77 publications

Eradication of Potato Virus S, Potato Virus A, and Potato Virus M From Infected in vitro-Grown Potato Shoots Using in vitro Therapies

Eradication of Potato Virus S, Potato Virus A, and Potato Virus M From Infected in vitro-Grown Potato Shoots Using in vitro Therapies

Shoot tip cryotherapy for plant pathogen eradication

Epigenetic and Genetic Integrity, Metabolic Stability, and Field Performance of Cryopreserved Plants

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