Growth, microcystin-production and proteomic responses of Microcystis aeruginosa under long-term exposure to amoxicillin

Liu, Ying; Shi, Chuang; Zhang, Jian; Gao, Baoyu

doi:10.1016/j.watres.2016.01.060

Cited by 70 publications

(23 citation statements)

References 42 publications

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“…As a starting point of the electron transport chain during photosynthesis, the photosystem II protein D1 (psbA) regulates various photosynthesis-related stress responses in cyanobacteria (Shao et al 2009). In addition to this study, previous studies also found that protein D1 regulated stress responses in M. aeruginosa exposed to amoxicillin and spiramycin (Liu et al 2015(Liu et al , 2016, suggesting that protein D1 might be a target protein of different antibiotics. In addition to the stimulation of the photosynthesis pathway of energy metabolism, the PPI functional modules in Fig.…”

Section: Cellular Metabolism After Antibiotic Exposuresupporting

confidence: 67%

“…Among all of the differentially expressed proteins, two highly connective hub proteins (superoxide dismutase and enolase) and D1 protein were commonly shared by M. aeruginosa exposed to ciprofloxacin, sulphamethoxazole and amoxicillin (Liu et al . ), suggesting these proteins were common action targets of different antibiotics in M. aeruginosa .…”

Section: Resultsmentioning

confidence: 97%

“…In addition to this study, previous studies also found that protein D1 regulated stress responses in M. aeruginosa exposed to amoxicillin and spiramycin (Liu et al . , ), suggesting that protein D1 might be a target protein of different antibiotics.…”

Section: Discussionmentioning

confidence: 99%

“…However, antibiotic contaminants have the potential to stimulate cyanobacterial growth and cyanotoxin production at concentrations lower than the toxic threshold (Liu et al . ). The stimulatory effects of antibiotics may promote harmful cyanobacterial bloom and require more attention.…”

Section: Introductionmentioning

confidence: 97%

“…Recent studies mainly examined the inhibitory effects of antibiotics and presented the toxic threshold below which cyanobacteria were supposed to be unaffected by antibiotics (Ando et al 2007). However, antibiotic contaminants have the potential to stimulate cyanobacterial growth and cyanotoxin production at concentrations lower than the toxic threshold (Liu et al 2016). The stimulatory effects of antibiotics may promote harmful cyanobacterial bloom and require more attention.…”

Section: Introductionmentioning

confidence: 99%

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Stimulation effects of ciprofloxacin and sulphamethoxazole in Microcystis aeruginosa and isobaric tag for relative and absolute quantitation‐based screening of antibiotic targets

et al. 2016

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Antibiotics are normally regarded as safe to aquatic ecosystems when their contamination concentrations are lower than the toxic threshold. This study observed the hazard of ciprofloxacin, sulphamethoxazole and their binary mixture to the aquatic environment at environmentally relevant concentrations lower than the toxic threshold, due to the stimulation on the bloom of Microcystis aeruginosa. The enhanced growth of M. aeruginosa, coupled with elevated photosynthesis activity, was exerted by 50-200 ng/L of ciprofloxacin, 100-200 ng/L of sulphamethoxazole and 20-100 ng/L of the binary antibiotic mixture. Stimulated production and release of microcystins were observed at even lower concentrations. The upregulation of transcription-related proteins, cell division-related proteins, a gas vesicle protein, a global nitrogen regulator (ntcA), two microcystin synthetases (mcyC and mcyH) and ATP-binding cassette transporters provided direct proteomic evidence for the regulation of target antibiotics on M. aeruginosa bloom. Cytochrome P450 was an essential component involved in stress responses and antibiotic biodegradation. Proteomic responses to antibiotic exposure presented a shift in the energy metabolism of M. aeruginosa towards the excitation of photosynthesis, an increase of carbohydrate biosynthesis and the inhibition of carbohydrate catabolism. Superoxide dismutase, enolase and D1 protein were candidate target proteins of different antibiotics in M. aeruginosa. The antibiotic mixture showed a greater hazard than single antibiotics, and a safe threshold of 5 ng/L was suggested for each target antibiotic under the coexistence condition.

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Section: Cellular Metabolism After Antibiotic Exposuresupporting

confidence: 67%

Section: Resultsmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations