Growth, metabolism and baculovirus production in suspension cultures of an Anticarsia gemmatalis cell line

Gioria, Verónica Viviana; Jäger, Volker; Claus, Juan Daniel

doi:10.1007/s10616-006-9042-3

Cited by 19 publications

(29 citation statements)

References 48 publications

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“…The DSIR-HA-1179 cell specific growth rate (l) was calculated by identifying the linear region from the semilog plot of viable cell density versus time, followed by linear regression of these data (Gioria et al 2006). Population doubling time (t d ) was calculated by Eq.…”

Section: Ratesmentioning

confidence: 99%

“…However, this process has several disadvantages, including the requirement for a facility for growing and maintaining the insects, the need for numerous personnel and a final product that suffers from inconsistencies in virus concentration and purity. In vitro large-scale production of OrNV in insect cell cultures would be an important step towards the feasible and robust production of this virus, provided that engineering and economical constrains are solved (Visnovsky et al 2003;Gioria et al 2006). The profitable production of viral biopesticides in vitro requires the efficient scale up of a host cell line, the use of low-cost chemically defined and preferably animalcomponent-free cell culture media, and the maintenance of high viral specific productivity (Claus et al 2012).…”

Section: Introductionmentioning

confidence: 99%

“…Metabolic profiles vary among insect cells lines. Glucose has been found to be the major carbohydrate source in a range of insect cell lines including Sf-9 (Reuveny et al 1992), HighFive (Rhiel et al 1997), saUFL-Ag-286 (Gioria et al 2006) and BCIRL-HZ-AM1 (Lua and Reid 2003). BTI-Tn-5B1-4 and BCIRl-HZ-AM1 cells display an inherent tendency to produce lactate and ammonia even when provided with sufficient glucose to grow in batch culture (Rhiel et al 1997;Lua and Reid 2003), while Sf-9 cells will produce them only under glucose deprivation (Ohman et al 1995;Drews et al 2000).…”

Section: Introductionmentioning

confidence: 99%

“…BTI-Tn-5B1-4 and BCIRl-HZ-AM1 cells display an inherent tendency to produce lactate and ammonia even when provided with sufficient glucose to grow in batch culture (Rhiel et al 1997;Lua and Reid 2003), while Sf-9 cells will produce them only under glucose deprivation (Ohman et al 1995;Drews et al 2000). On the other hand, the metabolism of the saUFL-AG-286 cell line is characterized by the production of ammonia but not of lactate (Gioria et al 2006). To date, no studies have been conducted on nutrient consumption or metabolite production by DSIR-HA-1179 cells.…”

Section: Introductionmentioning

confidence: 99%

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Characterization of growth and Oryctes rhinoceros nudivirus production in attached cultures of the DSIR-HA-1179 coleopteran insect cell line

et al. 2013

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The DSIR-HA-1179 coleopteran cell line is a susceptible and permissive host to the Oryctes rhinoceros nudivirus (OrNV), which has been used as a biocontrol agent against the coconut rhinoceros beetle (Oryctes rhinoceros); a pest of palms in the Asia-Pacific region. However, little is known about growth and metabolism of this cell line, knowledge of which is necessary to develop an in vitro large-scale OrNV production process. The strong anchoragedependent characteristics of the cell line, its particular fragility and its tendency to form dense clumps when manipulated, are the most likely reasons that have precluded further development of the cell line. In order to characterize DSIR-HA-1179 cells, there was first a need for a reliable technique to count the cells. A homogenous cell suspension suitable for enumeration could be produced by treatment with TrypLE Express TM with optimum mean time for cell release calculated as 30 min. The cell line was adapted to grow in four serum-supplemented culture media namely TC-100, IPL-41, Sf-900 II and Sf-900 III and cell growth, glucose consumption, lactate and ammonia production were assessed from static-batch cultures. The maximum viable cell density was reached in Sf-900 II (17.9 9 10 5 cells/ml), with the maximum specific growth rate observed in this culture medium as well (0.0074 h -1 ). Higher production of OrNV was observed in IPL-41 and TC-100 (4.1 9 10 7 TCID 50 /ml) than in cultures infected in Sf-900 III (2.0 9 10 7 TCID 50 /ml) and Sf-900 II (1.4 9 10 7 TCID 50 /ml). At the end of the growth period, glucose was completely consumed in cultures grown in TC-100, while remained in excess in the other three culture media. The cell line produced lactate and ammonia to very low levels in the TC-100 culture medium which is a promising aspect for its cultivation at large-scale.

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Section: Ratesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Characterization of growth and Oryctes rhinoceros nudivirus production in attached cultures of the DSIR-HA-1179 coleopteran insect cell line

et al. 2013

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“…This procedure allowed that after 20-30 passages most of the Se301 cells were growing as single cells. This protocol was also followed by Gioria et al (2007), which permitted the adaptation of the UFL-AG-286 cell line from Anticarsia gemmatalis to suspension cultures, even though large clumps were formed when agitation was initiated.…”

Section: Influence Of the Protective And Disaggregant Additives On Thmentioning

confidence: 99%

Adaptation of the Se301 insect cell line to suspension culture. Effect of turbulence on growth and on production of nucleopolyhedrovius (SeMNPV)

et al. 2011

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As chemical pesticides are being banned as control agents for agricultural pests, the use of the highly specific, safe to non-target organisms baculoviruses has been proposed. These viruses can be produced either in vivo or in vitro. In vitro production requires appropriated host insect cell lines with the ability for growing as freely-suspended cells. In this work, the Spodoptera exigua Se301 cell line was used to produce the commercially available S. exigua nucleopolyhedrovirus (SeMNPV) in suspension. Se301 cells showed to be very sensitive to the hydrodynamic shear rates developed in bioreactors. A process of progressive adaptation to freely-suspended cultures using protective additives against shear stress and disaggregant was proposed. The best combinations were polyvinyl alcohol (PVA) or polyvinyl pyrrolidone (PVP) with the disaggregant dextran sulfate (DS). Both static and freely-suspended Se301 cell cultures were successfully infected with the SeMNPV baculovirus. Production of occluded baculovirus (OB) increased with the multiplicity of infection (MOI [ 0.1).

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Virus Production Under Suspension Conditions

Merten

Bakker

Vorlop

et al. 2014

Industrial Scale Suspension Culture of Living Cells

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Growth, metabolism and baculovirus production in suspension cultures of an Anticarsia gemmatalis cell line

Cited by 19 publications

References 48 publications

Characterization of growth and Oryctes rhinoceros nudivirus production in attached cultures of the DSIR-HA-1179 coleopteran insect cell line

Characterization of growth and Oryctes rhinoceros nudivirus production in attached cultures of the DSIR-HA-1179 coleopteran insect cell line

Adaptation of the Se301 insect cell line to suspension culture. Effect of turbulence on growth and on production of nucleopolyhedrovius (SeMNPV)

Virus Production Under Suspension Conditions

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