Growth kinetics, polyamine pattern and biosynthesis in hairy root lines of <i>Nicotiana tabacum</i>

Mengoli, Marisa; Ghelli, Anna; Chriqui, Dominique; Bagni, Nello

doi:10.1111/j.1399-3054.1992.tb04773.x

Cited by 12 publications

(6 citation statements)

References 28 publications

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“…Previous work showed that polyamine accumulation could be reduced genetically by introducing the Ri TL-DNA (or genes there from) into the plant genome [17][18][19][20][21]. Furthermore, the phenotypic changes associated with these genes in the aerial parts were mimicked by treatment of whole plants with DFMO [12].…”

Section: Resultsmentioning

confidence: 99%

Changing root system architecture through inhibition of putrescine and feruloyl putrescine accumulation

et al. 1994

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Plant roots provide anchorage and absorb the water and minerals necessary for photosynthesis in the aerial parts of the plant. Since plants are sessile organisms, their root systems must forage for resources in heterogeneous soils through differential branching and elongation [(1988) Funct. Ecol. 2, 345-351;(1991) , in a model system consisting of excised tobacco roots, that both a-or.-difluoromethylornithine (an inhibitor of putrescine biosynthesis) and the rolA gene (from the root-inducing transferred DNA of Agrobacterium rhizogenes) stimulate overall root growth and cause a conversion in the pattern of root system formation, producing a dominant or 'tap' root. These morphological changes are correlated with a depression in the accumulation of polyamines and their conjugates.

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Section: Resultsmentioning

confidence: 99%

Changing root system architecture through inhibition of putrescine and feruloyl putrescine accumulation

et al. 1994

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“…In this study the presence of agropin in hairy roots confirms the genetic transformation of these tissues [22]. The synthesis of this unusual amino acid occur as a result of the transition of T-DNA segment of Ri plasmid containing the genes controlled the agropin synthesis [23].The rapid growth of these transformed hairy roots and its dense of root hairs, may explained to the length of apical meristem of these roots [19], as well as to the increased rates of cell division [24]. The conclusion is transformed hairy roots represent an efficient alternative approach to regenerate into plants, often spontaneously, such as occur in Brassica oleracea [25] and in Solanum nigrum [26].…”

Section: Discussionmentioning

confidence: 78%

Genetically transformed hairy roots producing agropine induced on Trigonella foenum-graecum L. plant by Agrobacterium rhizogenes 1601.

Al-Mahdawe¹,

Al-Mallah²,

Al-Attrakchii³

2013

JoBRC

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Inoculation of leaves and stems explants excised from field-grown Trigonella foenum-graecum L. with engineered Agrobacterium rhizogenes 1601 inoculum of optical density 1.90, 2.06 and 1.96 led to the formation of hairy roots on these explants. The highest percent of infection with inoculum of O.D 1.90 was 20% in leaves, and was 53.3% in stems using inoculum of O.D 2.06. Infection percent was 50% in hypocotyl compared with non-inoculated explants. The results showed that inoculation of seedlings lacking roots were slow in hairy root formation and take long time compared with their formation in intact seedlings inoculated with the same inocula. Results of paper electrophoresis of these root proved the incidence of their genetic transformation. Moreover, these transformed roots grow happily in liquid WP medium with an average fresh weight 5.9g after four weeks of culture, whereas fresh weight of normal roots was 1.02g under the same conditions.

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“…B, C and D loci, corresponding to ORFs 10, 11, 12 and 15 of the TL-DNA, were found to be involved in the control of hairy root induction (White et al 1985), The rolA, rolB and rolC genes individually are able to induce roots on leaf explants of tobacco (Spena et al, 1987) but differ in their capacity to stimulate root formation in different hosts and have distinct effects on root growth (Schmulling et al, 1988), The Ri TL-DNA modifies development through poorly understood mechanisms. Root induction and morphological abnormalities, due to the presence and expression of the Ri TL-D.NA, are linked to an altered metabolism of planl hormones (Scliniiilling et al 1993) and polyamines (Martin-Tanguy et al, 1990, Mengoli et al 1992a.…”

Section: Introductionmentioning

confidence: 99%

“…However, altered polyamine levels aod metabolism are reported to be associated with the presence of Ri TL-DNA. For instance, free and conjugated polyamine levels decrease in roots and shoot tips of transformed tobacco plants (T), and diminish even more as phenotypic transformation increases (T' plants) (Martin-Tanguy et al 1990, Mengoli et al 1992a). In transformed plants.…”

Section: Introductionmentioning

confidence: 99%

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Effect of the rol genes from Agrobacterium rhizogenes on polyamine metabolism in tobacco roots

Altabella¹,

Angel²,

Biondi³

et al. 1995

Physiol Plant

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1995. Effect of the rol genes from Agrobacterium rhizogenes on polyamine metabolism in tobacco roots. -Physiol. Plant. 95: 479^85.Using tobacco root cultures, we explored the possibility of a coirelation between the expression of the rol genes from the TL-DNA (rolA. rolB, rolC) of Agrobacterium rhizogenes and changes in polyamine content and metabolism. Transgenic roots were induced by inoculation of Nicotiana tabacum cv. Xanthi leaf discs with a disarmed strain of i4. tumefaciens harbouring the rolA. rolB and rolC genes either singly or in combination: the presence of these genes on the plant genome was confirmed by Southern blot analysis. Transgenic roots, especially those transformed either by a combination of the three rol genes (A-i-B-i-C) or the rotC alone, grew faster than the untransformed roots. Putrescine, spennidine and traces of spermine were present in all samples, both in free and hound forms. While roiA roots showed increased levels of free and bound polyamines as compared with controls, accumulation of polyamines in rolB and rolC roots was inhibited or mantained, with the exception of a 66 and 48% increase, respectively, in the PCA-soluble conjugated fraction. In roots transgenic for all three rol genes (A+B-fC), the poiyamine content remained almost unaltered compared with controls, suggesting that rolB and rolC genes could nullify or compensate the roiA effects. The higher polyamine contents found in roots transformed by rolA paralleled with higher omithine (EC 4.1.1.17) and arginine (EC 4.1.1.19) decarboxylase activities as well as higher nicotine production, it is suggested that polyamine metabolism in root cultures is altered by expression of rol genes.

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Growth kinetics, polyamine pattern and biosynthesis in hairy root lines of Nicotiana tabacum

Cited by 12 publications

References 28 publications

Changing root system architecture through inhibition of putrescine and feruloyl putrescine accumulation

Changing root system architecture through inhibition of putrescine and feruloyl putrescine accumulation

Genetically transformed hairy roots producing agropine induced on Trigonella foenum-graecum L. plant by Agrobacterium rhizogenes 1601.

Effect of the rol genes from Agrobacterium rhizogenes on polyamine metabolism in tobacco roots

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