Growth kinetics of progenitor cell-enriched hematopoietic cell populations in long-term liquid cultures under continuous removal of mature cells

Flores-Guzmán, Patricia; Martı́nez-Jaramillo, Guadalupe; Montesinos, Juan José; Valencia, Ignacio; Mayani, Héctor

doi:10.1080/14653240600735776

Cited by 17 publications

(11 citation statements)

References 30 publications

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“…1A). It has to be kept in mind that newborn and adult hematopoietic populations had similar levels of both CD34 ϩ cells and CFCs at culture onset, as well as on days 7, 14, and 21 (13). Thus, confirming previous studies by our own group and others, results shown in Fig.…”

Section: Proliferation Analysissupporting

confidence: 93%

“…2A Both cell fractions were cultured in serum-free liquid cultures supplemented with a combination of seven stimulatory cytokines. On days 0, 7, 14, and 21, Lin Ϫ cells were obtained by negative selection and cultured for 7 days, as described previously (13). Results shown represent the increase in total cell number (A) or the proportion of cells in each phase of cell cycle (B) on the indicated days of culture, and correspond to mean Ϯ SD of 16 (UCB) and 7 (aMPB) separate experiments.…”

Section: Cell Cycle Statusmentioning

confidence: 99%

“…All cytokines were obtained from STI and were added at 10 ng/ml. The whole procedure was performed as described in detail elsewhere (13).…”

Section: Liquid Culturesmentioning

confidence: 99%

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Cell Cycle Differences in Vitro between Primitive Hematopoietic Cell Populations from Adult and Umbilical Cord Blood

Alvarado-Moreno

Chávez‐González

Cérbulo

et al. 2007

Stem Cells and Development

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Lineage-negative (Lin(-)) cell populations, obtained by negative selection from umbilical cord blood (UCB) and adult mobilized peripheral blood (aMPB), were cultured in serum-free liquid cultures supplemented with a mixture of seven stimulatory cytokines. On specific days, proliferation potential was assessed and cell cycle status was determined by DNA content. Expression of the cell cycle regulators cyclin D3 (cD3), cyclin-dependent kinase 4 (cdk4), p21(cip1/waf1) (p21), and p27(kip1) (p27) was also determined. As expected, UCB cells showed significantly higher proliferation potentials than aMPB cells, particularly during the first 7 days of culture. During this period of time, higher numbers of cell cycles were observed in UCB cells (7-9 cycles), as compared to aMPB cells (5-6 cycles). Higher levels of cD3, cdk4, and p27 were also detected in UCB cells. Our results confirm that UCB cells possess an intrinsically higher proliferation potential, as compared to aMPB cells, and suggest that such a biological difference is due, at least in part, to differences in cell cycle status. This, in turn, seems to result from the differential expression of cell cycle regulatory molecules.

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Section: Proliferation Analysissupporting

confidence: 93%

Section: Cell Cycle Statusmentioning

confidence: 99%

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Cell Cycle Differences in Vitro between Primitive Hematopoietic Cell Populations from Adult and Umbilical Cord Blood

Alvarado-Moreno

Chávez‐González

Cérbulo

et al. 2007

Stem Cells and Development

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“…For example, Population V (>98% CD34+ cells) showed significantly higher potentials than Population III (74% CD34+ cells); Population III, in turn, showed significantly higher potentials than Population I (1% CD34+ cells). We and others have previously shown that the presence of precursor and mature cells in culture—particularly those of the monocyte‐macrophage lineage—may have negative impacts on the growth of HSCs and HPCs, since the accessory cells consume nutrients and cytokines present in the medium, and may produce or elicit the production of soluble factors that limit the growth of primitive cells 30‐34 . Thus, it seems very likely that the limited proliferation‐expansion observed in Population I, and to a lesser extent in Populations II and III, was due to the presence of such accessory cells.…”

Section: Discussionmentioning

confidence: 99%

Comparative in vitro analysis of different hematopoietic cell populations from human cord blood: in search of the best option for clinically oriented ex vivo cell expansion

et al. 2012

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“…Previous reports have cited instances where T-lymphocytes, 22,23 natural killer (NK) cells, 24,25 macrophages 26,27 and other less well defined “accessory” cells 28,29 have been shown to negatively impact hematopoiesis. Phenotypic analysis of CB revealed that the major cellular components are CD3 + , CD14 + , CD56 + and CD19 + cells and each was investigated (alone and in combination) with respect to their potential as inhibitory “accessory” cells influencing the magnitude of CD34 + ex vivo expansion achieved.…”

Section: Introductionmentioning

confidence: 99%

CD3+ and/or CD14+ depletion from cord blood mononuclear cells before ex vivo expansion culture improves total nucleated cell and CD34+ cell yields

Yang

Robinson

et al. 2009

Bone Marrow Transplant

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Cord blood (CB) is used increasingly in transplant patients lacking sibling or unrelated donors. A major hurdle to the use of CB is low cell dose which is largely responsible for elevated risk of graft failure and significantly delayed neutrophil and platelet engraftment. Since a positive correlation has been demonstrated between the total nucleated cell (TNC) and CD34+ cell dose transplanted and time to neutrophil and platelet engraftment, strategies to increase these measures are under development. One strategy includes the ex vivo expansion of CB mononuclear cells (MNC) with mesenchymal stem cells (MSC) in a cytokine cocktail. We demonstrate that this strategy can be further improved if CD3+ and/or CD14+ cells are first depleted from the CB MNC prior to ex vivo expansion. Ready translation of this depletion strategy to improve ex vivo CB expansion in the clinic is feasible since clinical-grade devices and reagents are available. Ultimately the goal of improving TNC and CD34+ transplant doses is to further improve the rate of neutrophil and platelet engraftment in CB recipients.

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Growth kinetics of progenitor cell-enriched hematopoietic cell populations in long-term liquid cultures under continuous removal of mature cells

Cited by 17 publications

References 30 publications

Cell Cycle Differences in Vitro between Primitive Hematopoietic Cell Populations from Adult and Umbilical Cord Blood

Cell Cycle Differences in Vitro between Primitive Hematopoietic Cell Populations from Adult and Umbilical Cord Blood

Comparative in vitro analysis of different hematopoietic cell populations from human cord blood: in search of the best option for clinically oriented ex vivo cell expansion

CD3+ and/or CD14+ depletion from cord blood mononuclear cells before ex vivo expansion culture improves total nucleated cell and CD34+ cell yields

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