Growth kinetics of gold nanoparticles on silica/graphene surfaces for multiplex biological immunoassays

Abstract

Kinetics of seed-mediated chemical growth of gold nanoparticles on substrates coated with a thin layer of a number of graphene materials were studied with ultimate goal of employing the developed graphene-nanoparticle composites in light-scattering imaging biosensor applications. The type of the surface coating and chemical conditions influence the surface number density and size of the grown nanoparticles. Importantly, when used as biosensor base, the substrates coated with protein reduced graphene oxide have shown beneficial biological compatibility towards immobilized antibodies resulting in about 20-fold improved antigen capture immunoassay performed on the multiplex photonic biosensor platform.