Growth Kinetics of Free and Immobilized Insect Cell Cultures^a

Effect of temperature and oxygen on cell growth and recombinant protein production in insect cell cultures

“…Insect cells can be successfully propagated at a temperature range of 25-30 ° C (Agathos et al 1990;Vaughn 1984); however, the optimal temperature range for cell propagation and viral infection is considered to be 27-28°C (Agathos et al 1990;Vaughn 1984;Stockdale and Priston 1981). Hink and Strauss (1976) reported that the percentage of vitally infected Trichoplusia ni cells and the number of wild-type Autographa californica nuclear polyhedrosis (AcNP) viral polyhedra produced per cell was a function of incubation temperature.…”

Kinetic analysis of in vitro production of wild-type Spodoptera frugiperda nucleopolyhedrovirus

“…Because of advances in cell culture, the production of baculoviruses in insect cells can be achieved in different growth systems, from monolayer static culture to suspension culture using Erlenmeyer flasks, spinners or bioreactors. Most insect cells can be cultured at 25-30°C (Agathos et al, 1990); however, the optimal temperature during cell growth and the infection of Sf9 and Sf21 cell lines is 28°C (King and Possee, 1992). Suspension cultures favor the study of kinetic variables of cell growth and protein production.…”

Requirements for baculoviruses for clinical gene therapy applications

“…Increasing the perfusion capability of the bioreactor to provide sufficient medium change/ input and to prevent nutrient limitations allows cell growth at higher densities. Different types of bioreactors, such as fed-batch, stirred tank (reviewed in Agathos et al (1990) and Aucoin et al (2010)) or disposable wave bioreactors (Vicente et al, 2009a) have successfully been used with insect cell/baculovirus expression systems. In addition, medium supplementation with specialized nutrient mixtures, containing glucose, glutamine (Ikonomou et al, 2003), pyruvate or a-ketoglutarate (Carinhas et al, 2010) has supported the growth of insect cells at high cell densities.…”