Growth kinetics of a Vero cells adapted Bangladeshi strain of peste des petits ruminants (PPR) virus in cell culture

Siddiqui, Saiful Islam; Islam, Rafiqul; Chowdhury, Emdadul Haque

doi:10.1007/s00203-020-02154-w

Cited by 3 publications

(2 citation statements)

References 20 publications

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“…However, in current study the virus was adapted in vero cells only in which the growth kinetics study was performed. The suggested optimum time point for harvesting the CDV based on current study is culture is 72 hpi which is similar to previous findings (Siddiqui et al, 2021) [20] . In growth curve there was a difference in the maximum titre achieved at 0.01 moi.…”

Section: Discussionsupporting

confidence: 90%

Virological and molecular characterization of canine distemper virus passaged at high temperature towards development of thermostable vaccine candidate

Einstien,

Rajak,

Bhatt

et al. 2024

Int. J. Adv. Biochem. Res.

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Thermolabile nature of viruses is a major problem faced by most live attenuated vaccines. Addressing this issue will help increase the success rate of vaccination and reduce the economic burden on vaccination campaigns. In current study, the Canine Distemper Virus (CDV) belonging to India-1/Asia-5 lineage was adapted to grow at 41.5oC for assessing its suitability as thermostable vaccine candidate. The growth kinetics of thermotolerent virus was compared to that of original parent virus and it was found that the thermotolerent virus could grow only to a lower titre (4.5 logTCID50 per ml) in Vero cells as compared to the parent virus (5.61 logTCID50 per ml). When the coding sequences of structural genes of the virus M, N, H and F were compared before and after the high temperature passage, it was found that the H gene accrued the highest number of changes as compared to other genes of CDV. The mapped mutations in DNA and substitutions in amino acid sequences may shed light on the structural and functional mechanisms of virus adaptation at high temperature to be used as a strategy for creating recombinant thermostable vaccines.

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Section: Discussionsupporting

confidence: 90%

Virological and molecular characterization of canine distemper virus passaged at high temperature towards development of thermostable vaccine candidate

Einstien,

Rajak,

Bhatt

et al. 2024

Int. J. Adv. Biochem. Res.

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“…At present, CPB cells below 120 passages are prone to mutation, apoptosis, and are not stable. Compared with Vero and MDCK cells, the proliferative capacity of CPB cells was slightly weaker [ 22 , 23 ]. The instability and low productive capacity of CPB p100 cells influenced the development of the vaccine production process and hindered the marketing of ISKNV, SCRV, and LMBV vaccines.…”

Section: Introductionmentioning

confidence: 99%

The Stability and Efficency of CPB Cells Were Acclimated for Virus Proliferation

Niu,

Ma,

Liang

et al. 2024

Vaccines

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Background: Vaccinations are still the most effective means of preventing and controlling fish viral diseases, and cells are an important substrate for the production of a viral vaccine. Therefore, the rapid-stable growth and virus sensitivity of cells are urgently needed. Methods: Chinese perch brain 100th passage (CPB p100) were acclimated in a low serum with 5% FBS L-15 for 50 passages, then transferred to 8% FBS L-15 for 150 passages. Additionally, the morphology and cell type of CPB 300th passage (CPB p300) cells were identified. We analyzed the transfection efficiency and virus sensitivity of CPB p300 cells, and then optimized the conditions of ISKNV, SCRV, and LMBV multiplication in CPB cells. Results: CPB p300 cells were more homogeneous, and the spread diameter (20–30) µm in CPB p300 cells became the dominant population. The doubling time of CPB p300 was 1.5 times shorter than that of CPB p100.However, multiplication rate of CPB p300 was 1.37 times higher than CPB p100. CPB p300 cells were susceptible to ISKNV, SCRV, and LMBV, and the optimal conditions of ISKNV, SCRV, and LMBV multiplication were simultaneous incubation, 0.6 × 105 cells/cm2 and MOI = 0.1; infection at 48 h, 0.8 × 105 cells/cm2 and MOI = 0.01; simultaneous incubation, 0.7 × 105 cells/cm2 and MOI = 0.05, respectively. The time and economic costs of ISKNV, SCRV, and LMBV multiplication in CPB p300 cells were significantly reduced. Conclusions: The acquisition of CPB p300 cells laid a good material foundation for the production of ISKNV, SCRV, and LMBV vaccines.

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Coinfection kinetics of goatpox virus and peste-des-petits-ruminants virus in Vero cells

et al. 2022

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Growth kinetics of a Vero cells adapted Bangladeshi strain of peste des petits ruminants (PPR) virus in cell culture

Cited by 3 publications

References 20 publications

Virological and molecular characterization of canine distemper virus passaged at high temperature towards development of thermostable vaccine candidate

Virological and molecular characterization of canine distemper virus passaged at high temperature towards development of thermostable vaccine candidate

The Stability and Efficency of CPB Cells Were Acclimated for Virus Proliferation

Coinfection kinetics of goatpox virus and peste-des-petits-ruminants virus in Vero cells

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