Growth kinetics, antigen profiling, and proteinase activity of Egyptian Trichomonas tenax isolates derived from patients having oral infections

Sibaei, Mahmoud M. El; Abdel-Fattah, Nashwa S.; Ahmed, Sayeda S.; Abou-Seri, Hanan Mahmoud

doi:10.1016/j.exppara.2012.01.018

Cited by 26 publications

(19 citation statements)

References 32 publications

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“…Several recent studies have also indicated the parasitic potential of oral trichomonads, and question their prior description as commensal organisms (El Sibaei et al. ; Ribeiro et al. ; Yamamoto et al.…”

Section: Discussionmentioning

confidence: 99%

“…In the human mouth, trichomonads are commonly detected with prevalence rates varying between 12.7% and 37%, showing associations to periodontal disease (Athari et al 2007;Cuevas et al 2008;Dudko and Kurnatowska 2007). Several recent studies have also indicated the parasitic potential of oral trichomonads, and question their prior description as commensal organisms (El Sibaei et al 2012;Ribeiro et al 2015;Yamamoto et al 2000).…”

Section: %mentioning

confidence: 98%

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The Prevalence of Canine Oral Protozoa and Their Association with Periodontal Disease

Patel

Colyer²,

Harris³

et al. 2016

J Eukaryotic Microbiology

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Periodontal disease is one of the most important health concerns for companion animals. Research into canine forms of periodontitis has focused on the identification and characterization of the bacterial communities present. However, other microorganisms are known to inhabit the oral cavity and could also influence the disease process. A novel, broad spectrum 18S PCR was developed and used, in conjunction with next-generation sequencing analyses to target the identification of protists. Trichomonas sp. and Entamoeba sp. were identified from 92 samples of canine plaque. The overall prevalence of trichomonads was 56.52% (52/92) and entamoebae was 4.34% (4/92). Next-generation sequencing of pooled healthy, gingivitis, earlystage periodontitis, and severe periodontitis samples revealed the proportion of trichomonad sequences to be 3.51% (health), 2.84% (gingivitis), 6.07% (early periodontitis), and 35.04% (severe periodontitis), respectively, and entamoebae to be 0.01% (health), 0.01% (gingivitis), 0.80% (early-stage periodontitis), and 7.91% (severe periodontitis) respectively. Both genera of protists were statistically associated with plaque from dogs with periodontal disease. These findings provide the first conclusive evidence for the presence of oral protozoa in dog plaque and suggest a possible role for protozoa in the periodontal disease process.

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Section: Discussionmentioning

confidence: 99%

Section: %mentioning

confidence: 98%

The Prevalence of Canine Oral Protozoa and Their Association with Periodontal Disease

Patel

Colyer²,

Harris³

et al. 2016

J Eukaryotic Microbiology

View full text Add to dashboard Cite

show abstract

“…tenax is still discussed as a commensal organism, although a relationship between the increased occurrence of this protozoan and progression of periodontal disease has been described [1][2][3][4]. The high frequency of T. tenax detected in humans with pathological changes in the oral cavity, along with the variability in protein profiling and proteolytic activity supports the pathogenic nature of T. tenax [21,40].…”

Section: Discussionmentioning

confidence: 99%

Molecular identification of Trichomonas tenax in the oral environment of domesticated animals in Poland – potential effects of host diversity for human health

Dybicz¹,

Perkowski²,

Baltaza³

et al. 2018

Ann Agric Environ Med.

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It was proved that T. tenax, considered so far as a human specific parasite, can also inhabit the oral cavity of dog, cat and horse. To summarize, T. tenax was detected in the mouths of different domesticated animals, indicating that in Poland it can colonize a wider range of hosts than previously known. The owners of 3 dogs showed oral tissue inflammation of different intensity and were also positive for T. tenax; therefore, oral trichomonosis spread from humans to domestic animals and conversely should be taken into consideration.

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“…Authors have detected the capacity of T. tenax ATCC 30207 to adhere to diverse cells and induce cytotoxicity [17], while others studies showed no adherence or cytotoxic property [18][19]. Moreover, flagellates produce numerous enzymes, such as cysteine protease, cathepsin B like protein, haemolysins, metalloproteinase and other proteinases that could participate in the breakdown of collagen, gelatin observed in periodontal diseases [20][21][22][23][24][25]. Live trophozoites of the Hs-4 strain of T. tenax failed to induce secretion of IL-1β, IL-8, IL-10 or TNFα from macrophages.…”

Section: Characteristics Of Included Studiesmentioning

confidence: 99%

“…The authors have identified some of them and assessed their properties. In 1991, Bózner and Demes, and more recently El Sibaei, identified a temperature-dependant proteinase capable of degrading types I, III, IV and V collagen, that would be cysteine proteinases or metallo-proteinases [20,24,25]. These enzymes are cell-associated and extracellular proteinases released from alive T. tenax [25].…”

Section: The Postulate "Virulence Factors"mentioning

confidence: 99%

Assessment of the role of Trichomonas tenax in the etiopathogenesis of human periodontitis: A systematic review

2019

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ObjectiveThis systematic review was to assess the presence of Trichomonas tenax in patients with periodontitis and to elucidate its potential role in the onset and development of this disease. ConclusionsThis systematic review has reported the presence of T. tenax more frequently in diseased than healthy sites and the capacity of this flagellate to synthesis enzymes which could participate to the degradation of periodontal tissues. Nevertheless, these data do not meet all the postulates and are not enough to provide firm conclusions about the role of T. tenax in the etiopathogenesis of periodontitis.Trichomonas in periodontitis PLOS ONE | https://doi.

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Growth kinetics, antigen profiling, and proteinase activity of Egyptian Trichomonas tenax isolates derived from patients having oral infections

Cited by 26 publications

References 32 publications

The Prevalence of Canine Oral Protozoa and Their Association with Periodontal Disease

The Prevalence of Canine Oral Protozoa and Their Association with Periodontal Disease

Molecular identification of Trichomonas tenax in the oral environment of domesticated animals in Poland – potential effects of host diversity for human health

Assessment of the role of Trichomonas tenax in the etiopathogenesis of human periodontitis: A systematic review

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