Growth-inhibitory effect of hexa-N-acetylchitohexaose and chitohexaose against Meth-A solid tumor.

Tokoro, Akio; Tatewaki, Nobuhiko; Suzuki, Ko; Mikami, Takeshi; Suzuki, Sh.; Suzuki, Masuko

doi:10.1248/cpb.36.784

Cited by 260 publications

(100 citation statements)

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“…Previously, we have reported that NACOS-6 shows a growth-inhibitory effect against sarcoma-180, MM-46, and Meth-A solid tumors (21,25). In the present paper, we investigated whether NACOS-6-treated tumor-bearing mice could resist infection by one of the opportunistic fungi, C. albicans.…”

Section: Discussionmentioning

confidence: 92%

“…It should be noted that the most favorable condition for manifestation of antitumor effects by administration of NACOS-6, i.e., a single i.v. injection (25), is not the most favorable condition for evoking the capability to protect against infection, i.e., multiple injection via the same route. The latter procedure seems to be indispensable for induction of a strong resistance to C. albicans in mice, because all NACOS-6-untreated tumorbearing mice died within I week after inoculation with C. albicans.…”

Section: Discussionmentioning

confidence: 99%

“…A water-soluble oligosaccharide, N-acetylchitohexaose (NACOS-6), has been shown to increase the number of peritoneal exudate cells (19), to induce active oxygen-generating activity (20), and to attract human peripheral blood neutrophils by activating their arachidonate metabolic system (24). Furthermore, NACOS-6 has been shown to exhibit significant growth-inhibitory effects against sarcoma-180, MM-46 (21), and Meth-A solid tumors (25), to augment the release of IL-1 and IL-2, to elevate the DTH response to tumor cells (25), and to enhance the IL-2 response to lymphocytes (26). Additionally, NACOS-6 has also been found to display protective effects in mice against infection with Pseudomonas aeruginosa (23) and Listeria monocytogenes (27).…”

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confidence: 99%

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Effect of N‐Acetylchitohexaose against Candida albicans Infection of Tumor‐Bearing Mice

Kobayashi

Watanabe

Suzuki

et al. 1990

Microbiology and Immunology

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A water-soluble oligosaccharide, N-acetylchitohexaose (NACOS-6), was able to enhance the protective effect against Candida albicans infection in mice during the early phase of tumor-bearing. A significant decrease in the number of C. albicans cells in the kidneys of NACOS-6-treated tumor-bearing mice was observed 8 days after the fungal infection, or 15 days after the tumor transplantation. The candidacidal activity of polymorphonuclear leukocytes from NACOS-6-treated tumorbearing mice did not differ from that of NACOS-6-untreated tumor-bearing mice. On the other hand, the candidacidal activities of both macrophages and T lymphocytes increased following administration of NACOS-6 in the early phase of tumorbearing. The culture supernatant of T lymphocytes from NACOS-6-treated tumorbearing mice also potentiated the candidacidal activity of casein-induced macrophages. An enhancement of natural killer cell activity of splenic lymphocytes obtained from NACOS-6-treated tumor-bearing mice was also observed.

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Section: Discussionmentioning

confidence: 92%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

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Effect of N‐Acetylchitohexaose against Candida albicans Infection of Tumor‐Bearing Mice

Kobayashi

Watanabe

Suzuki

et al. 1990

Microbiology and Immunology

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“…6). These catalytic properties may be advantages in the efficient production of Nacetyl-chitooligosaccharides with biological activity (30,31) using organic solvents as reaction media.…”

Section: Discussionmentioning

confidence: 99%

Different Cleavage Specificities of the Dual Catalytic Domains in Chitinase from the Hyperthermophilic Archaeon Thermococcus kodakaraensis KOD1

Tanaka¹,

Fukui²,

Imanaka³

2001

Journal of Biological Chemistry

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The chitinase from the hyperthermophilic archaeon Thermococcus kodakaraensis KOD1, Tk-ChiA, has an interesting multidomain structure containing dual catalytic domains and triple chitin-binding domains. To determine the biochemical properties of each domain, we constructed deletion mutant genes corresponding to the individual catalytic domains and purified the recombinant proteins. A synergistic effect was observed when chitin was degraded in the presence of both catalytic domains, suggesting different cleavage specificity of these domains. Analyses of degradation products from N-acetyl-chitooligosaccharides and their chromogenic derivatives with thin layer chromatography indicated that the N-terminal catalytic domain mainly hydrolyzed the second glycosidic bond from the nonreducing end of the oligomers, whereas the C-terminal domain randomly hydrolyzed glycosidic bonds other than the first bond from the nonreducing end. Both catalytic domains formed diacetyl-chitobiose as a major end product and possessed transglycosylation activity. Further analysis of degradation products from colloidal chitin with high performance liquid chromatography showed that the N-terminal catalytic domain exclusively liberated diacetyl-chitobiose, whereas reactions with the C-terminal domain led to N-acetyl-chitooligosaccharides of various lengths. These results demonstrated that the N-terminal and C-terminal catalytic domains functioned as exo-and endochitinases, respectively. The biochemical results provide a physiological explanation for the presence of two catalytic domains with different specificity and suggest a cooperative function between the two on a single polypeptide in the degradation of chitin.

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“…It has become apparent that these oligomers have antibacterial, 1) antifungal,2) and antitumor 3 ) activities. There are a number of reports about bacteria degrading either chitin 4 ) or chitosan,5) but not so much information about bacteria degrading both chitin and chitosan in a single cell.…”

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confidence: 99%

Isolation, Identification, and Effect of Oxygen Supply on Cultivation of Chitin and Chitosan Degrading Bacterium

Yamasaki

Ohta

Morita

et al. 1992

Bioscience, Biotechnology, and Biochemistry

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Growth-inhibitory effect of hexa-N-acetylchitohexaose and chitohexaose against Meth-A solid tumor.

Cited by 260 publications

References 0 publications

Effect of N‐Acetylchitohexaose against Candida albicans Infection of Tumor‐Bearing Mice

Effect of N‐Acetylchitohexaose against Candida albicans Infection of Tumor‐Bearing Mice

Different Cleavage Specificities of the Dual Catalytic Domains in Chitinase from the Hyperthermophilic Archaeon Thermococcus kodakaraensis KOD1

Isolation, Identification, and Effect of Oxygen Supply on Cultivation of Chitin and Chitosan Degrading Bacterium

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