Growth inhibitory effect of ethanolic neem leaves extract on &lt;i&gt;Klebsiella, Salmonella&lt;/i&gt; and &lt;i&gt;Staphylococcus aureus&lt;/i&gt;

Banna, Quazi Rubyath; Parveen, Feroza; Iqbal, Md. Jalaluddin

doi:10.3329/bjp.v9i3.19454

Cited by 3 publications

(3 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Klebsiella at concentrations of 6.2 mg ml and 12.5 mg ml respectively. The average diameter of zone of inhibition was 18 mm for Klebsiella and 20 mm for Salmonella (Banna et al, 2014). It however failed to inhibit the growth of S. aureus at the highest -1 concentration used that is 50 mg ml .…”

Section: Resultsmentioning

confidence: 90%

Antibacterial activity of selected plants extract against pathogenic bacteria and detection of phytochemicals

Saxena¹,

Mukhopadhyay²,

Nandi³

2020

JEB

View full text Add to dashboard Cite

Aim: The aim of the present study was to assess the antibacterial activity of selected plants extract against Gram- positive and Gram- negative pathogenic bacteria. Methodology: Extracts of neem leaf (Azadirachta indica), rose petal (Rosa indica), amla fruit (Emblica officinalis) and clove buds (Syzygium aromaticum) were prepared in different solvents. Antibacterial activity against selected microorganisms was assayed using agar cup diffusion method, thereby measuring the zone of inhibition. The extracts with higher zone of inhibition were run on Thin Layer Chromatography (TLC) plates. The presence of phytochemicals was detected using iodine fumigation method followed by contact bioautography. Results: The extracts of neem leaf, rose petal, amla fruit and clove bud prepared in solvents like acetone, ethanol and ethyl acetate showed higher antibacterial effect against Staphylococcus aureus, Streptococcus pyogenes, Escherichia coli, Klebsiella pneumoniae and Salmonella typhimurium in agar cup diffusion assay. TLC separation of the extract of plant products showed dark brown bands of phytochemicals on silica-gel G 60 plate. Contact bioautography results showed maximum antibacterial activity against Klebsiella pneumoniae. Interpretation: The results of the present study provide scientific evidence for the traditional uses of Azadirachta indica, Rosa indica, Emblica officinalis and Syzygium aromaticum.

show abstract

Section: Resultsmentioning

confidence: 90%

Antibacterial activity of selected plants extract against pathogenic bacteria and detection of phytochemicals

Saxena¹,

Mukhopadhyay²,

Nandi³

2020

JEB

View full text Add to dashboard Cite

show abstract

“…The difference in results may occur because of the different concentrations and the purity of ethanol and methanol used. Banna et al [42] also investigated the antibacterial effect of neem seeds, flowers, and leaves against S. aureus, Salmonella, and Klebsiella. The growth inhibitory effects were found at a concentration of 6.2 mg/ml and 12.5mg/ml.…”

Section: Currently No Previous Research Has Been Reportedmentioning

confidence: 99%

Combined Effect of Honey, Neem (Azadirachta Indica), and Turmeric against Staphylococcus Aureus and E. Coli Isolated from a Clinical Wound Sample

Saleem

Sarfraz

Mazhar³

2022

BioSci Rev.

View full text Add to dashboard Cite

Antimicrobial resistance has become evident all over the world. Resistance to antibiotics has become a concern in case of a wide variety of bacterial species, both pathogenic and commensal. More recently, E. coli, pseudomonas, Staphylococcus aureus, Streptococcus, and Enterococci were found to be adversely affecting the healthcare structures of the world, particularly where acute and long-term care facilities are available. Microbial species were identified by Vitek compact-2 and MALDI-TOF, while antibiogram sensitivity was checked using Kirby Bauer disc diffusion and well diffusion method. The study used 20 wound samples from five (5) men and fifteen (15) women. Thirty-four (34) purified colonies of bacteria were created, in which 8 were E. coli and 2 were S. aureus. The effects of neem, turmeric, and honey with ethanol extracts showed the maximum zone of inhibition against clinically isolated E. coli, such as PM33C4 and AM25C4. While, methanol extract also showed the maximum zone of inhibition against PM56C4, AF34C4, and PM57C4, using disc diffusion and well diffusion methods. Correspondingly, the effect of neem, turmeric, and honey with ethanol extracts showed maximum inhibition against S. aureus. Whereas, methanol extract showed a sensitive zone of inhibition only against PM54C1 using the disc diffusion method. Hence, it was determined that natural ingredients such as honey, turmeric, and neem are an effective alternative to antibiotics because they manifest excellent antimicrobial activity against clinical bacterial isolates.

show abstract

“…Recent studies have shown that Neem possesses anti-inflammatory, anti-arthritic, antipyretic, hypoglycemic, antigastric ulcer, antibacterial, antifungal, and antitumor activities (7). It possesses a wide spectrum of antibacterial action against Gram-positive and Gram negative microorganisms (8).…”

Section: Introductionmentioning

confidence: 99%

Antibacterial Activity of Azadirachta indica Leaf Extracts Against Some Pathogenic Standards and Clinical Bacterial Isolates

Maleki¹,

Sadeghian-Rizi²,

Ghannadian³

et al. 2017

Avicenna J Clin Microbiol Infect

View full text Add to dashboard Cite

show abstract

Growth inhibitory effect of ethanolic neem leaves extract on <i>Klebsiella, Salmonella</i> and <i>Staphylococcus aureus</i>

Cited by 3 publications

References 8 publications

Antibacterial activity of selected plants extract against pathogenic bacteria and detection of phytochemicals

Antibacterial activity of selected plants extract against pathogenic bacteria and detection of phytochemicals

Combined Effect of Honey, Neem (Azadirachta Indica), and Turmeric against Staphylococcus Aureus and E. Coli Isolated from a Clinical Wound Sample

Antibacterial Activity of Azadirachta indica Leaf Extracts Against Some Pathogenic Standards and Clinical Bacterial Isolates

Contact Info

Product

Resources

About