Growth Inhibition of Various Pathogenic Microorganisms Using Effective Microorganisms (EM)

Safwat, Safwat M.; Rozaik, Ehab

doi:10.21276/ijre.2017.4.12.2

Cited by 6 publications

(4 citation statements)

References 4 publications

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“…spores was previously demonstrated to be a more accurate method of estimating the level of infection at the colony level [ 68 ], we applied this method to examine the therapeutic capacity of EM ® for bees as a dietary supplement. According to the results presented in this study, and the excellent results in other studies on biological and therapeutic effects for different disorders in animals, plants, and the environment [ 69 , 70 , 71 , 72 , 73 ], it should be acknowledged that EM ® holds a substantial potential for nosemosis treatment.…”

Section: Discussionsupporting

confidence: 55%

Effects on Some Therapeutical, Biochemical, and Immunological Parameters of Honey Bee (Apis mellifera) Exposed to Probiotic Treatments, in Field and Laboratory Conditions

Gajger

Vlainić

Šoštarić

et al. 2020

Insects

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Several negative factors contribute to a decline in the number of insect pollinators. As a novel approach in therapy, we hypothesize that the EM® for bees could potentially have an important therapeutic and immunomodulatory effect on honey bee colonies. The aim of our study was to evaluate its impact on honey bees at the individual and colony level. This is the first appliance of the commercial probiotic mix EM® PROBIOTIC FOR BEES in honey bees as economically important social insects. The sugar syrup with 10% of probiotic was administered by spraying or feeding the honey bee colonies in the field conditions, in order to evaluate the infection levels with spores of Nosema spp. and colonies’ strength. Moreover, in laboratory-controlled conditions, in the hoarding cages, adult workers have been fed with sugar syrup supplemented with 2.5, 5, and 10% of EM® for bees for biochemical and immunological analyses of hemolymph, and with 5 and 10% for measuring the size of hypopharyngeal glands. It was found that following the EM® for bees administration the Nosema spp. spore counts in colonies were significantly reduced, and colonies’ strength was increased. The results at the individual level showed significant positive physiological changes in treated groups of adult bees, revealing at the same time a higher mortality rate when feeding sugar syrup supplemented with the probiotic.

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Section: Discussionsupporting

confidence: 55%

Effects on Some Therapeutical, Biochemical, and Immunological Parameters of Honey Bee (Apis mellifera) Exposed to Probiotic Treatments, in Field and Laboratory Conditions

Gajger

Vlainić

Šoštarić

et al. 2020

Insects

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“…The current results support the results of other studies, which also indicated the efficacy of this product in inhibiting the growth of pathogenic bacteria within the same concentrations. Where an Egyptian study (28) pointed that the use of this product and within the previously mentioned concentrations 1% had a significant effect on the growth of nine types of pathogenic bacteria, including S. aureus and E. coli, even they pointed to use of this product as a disinfectant and also the possibility of using it as a cleaner for sewage, while a study in Thailand (29) indicated that the EM did not have a significant effect in reducing the numbers of Salmonella enterica and Campylobacter spp. when used in the fields of broiler.…”

Section: Discussionmentioning

confidence: 99%

“…The main role of the effective microorganisms in inhibiting the growth of pathogenic bacteria inside the host body is not definitively determined, but it is thought to compete with pathogenic bacteria on food inside the host body (8,9). In addition, its metabolic products are harmful to the growth of other bacteria, especially pathogens, due to contain Lactobacilli (Lactic acid bacteria) bacteria that produce lactic acid, which is considered a powerful sterilizer that inhibit the harmful bacteria (28,32). It also maintains or supports bacterial balance in the intestines (33), and also stimulates the specific and non-specific immune system (11).…”

Section: Discussionmentioning

confidence: 99%

The inhibitory role of effective microorganisms on the growth of pathogenic bacteria

Hamad¹,

Hussein²,

Mahmmoud³

et al. 2020

IJVS

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This study was conducted to evaluate the efficacy of Effective Microorganisms (EM1 ® ) for inhibiting the growth of some pathogenic bacteria Staphylococcus aureus and E. coli were used in this study and isolated from pathological conditions. These bacteria were diagnosed in laboratory of microbiology, College of Veterinary Medicine, University of Mosul. The colonies that taken from blood agar were 5-7 and cultured in the nutrient broth and incubated at 37 ºC for 24 hours. Bacterial growth was calibrated with the second tube of the McFarland tubes 0.5%. Several concentrations of EM product were prepared 1, 0.5, 0.25 and 0.125%. Decimal dilutions were done for each concentration of EM product with bacterial suspension, except control group was done for bacterial suspension with nutrient broth. The bacterial count was done on nutrient agar, milk agar and EMB agar. The results of this study showed that the product of EM1 ® within concentrations 0.5-1% was highly efficient in inhibiting the growth of pathogenic bacteria under study. The bacterial count of both S. aureus and E. coli was 54x10 7 and 52x10 7 CFU/ ml respectively at 1% EM1 ® , and 67x10 7 and 86x10 7 CFU/ ml respectively at 0.5%, while the counting of the control group was 42x10 9 and 67x10 9 CFU/ ml respectively. This study concluded that EM1 ® at low concentrations have a clear role in inhibiting the growth of pathogenic bacteria, particularly S. aureus and E. coli.

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“…or Escherichia coli. The antimicrobial laboratory tests conducted by Wroński et al (2010) and Safwat and Rozaik (2018) showed that EM can inhibit the growth of pathogenic bacteria such as Escherichia coli, Pseudomonas aeruginosa, and Streptococcus faecalis.…”

Section: Introductionmentioning

confidence: 99%

Archives of Environmental Protection

Krzysztof¹,

Pawelek²,

Dacewicz³

2020

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The study discusses an experimental method for treatment of high strength domestic sewage on biofi lters fi lled with polyurethane (PUR) waste in the form of trims of upholstery foam. We determined eff ectiveness of two biological preparations containing eff ective microorganisms in elimination of organic and biogenic compounds, indicator bacteria and total suspended solids from the sewage pretreated in a septic tank. After four months of work under a hydraulic loading of 76.4 mm•d -1 we found the fi lter with 60 cm foam layer to be the most effi cient in the elimination of BOD 5 , COD Cr , NH 4 + -N and coliform bacteria. An average reduction in these pollutants reached 79.4%, 67.8%, 58.0% and 88.0%, respectively. Vertical fi lters fi lled with trims of upholstery foam and supplied with eff ective microorganisms ensured favorable conditions for development of heterotrophic and nitrifying bacteria without any need for additional aeration.

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Growth Inhibition of Various Pathogenic Microorganisms Using Effective Microorganisms (EM)

Cited by 6 publications

References 4 publications

Effects on Some Therapeutical, Biochemical, and Immunological Parameters of Honey Bee (Apis mellifera) Exposed to Probiotic Treatments, in Field and Laboratory Conditions

Effects on Some Therapeutical, Biochemical, and Immunological Parameters of Honey Bee (Apis mellifera) Exposed to Probiotic Treatments, in Field and Laboratory Conditions

The inhibitory role of effective microorganisms on the growth of pathogenic bacteria

Archives of Environmental Protection

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