Growth inhibition of N1E-115 mouse neuroblastoma cells by C-myc or N-myc antisense oligodeoxynucleotides causes limited differentiation but is not coupled to neurite formation

Larcher, Jean Christophe; Basseville, Monique; Vayssière, Jean-Luc; Cordeau-Lossouarn, Laurence; Croizat, Bernard; Gros, François

doi:10.1016/0006-291x(92)91714-2

Cited by 20 publications

(10 citation statements)

References 20 publications

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“…14,15 In vitro, the N1E-115 cells undergo neuronal differentiation in response to dimethylsulfoxide (DMSO), adenosine 3 0 , 5 0 -cyclic monophosphate (cAMP), or serum withdrawal. [16][17][18][19][20] Upon induction of differentiation, proli- In a recent study, we have found that enrichment of a PLGA nerve scaffold with N1E-115 cells exerts negative effects on nerve fiber regeneration. 21 Since we hypothesized that the transplanted cells could have represented an impeding material inside the conduit which hindered the positive effect of local neurotrophic factor release, the purpose of the present study was to ascertain whether transplantation of in vitro predifferentiated N1E-115 cells supported by an equine type III collagen membrane wrapped around the site of a nerve crush injury might positively affect nerve regeneration.…”

Section: 2mentioning

confidence: 99%

Neural cell transplantation effects on sciatic nerve regeneration after a standardized crush injury in the rat

et al. 2008

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The goal of the present study was to assess whether in vitro-differentiated N1E-115 cells supported by a collagen membrane would enhance rat sciatic nerve regeneration after a crush injury. To set up an appropriate experimental model for investigating the effects of neural cell transplantation, we have recently described the sequence of functional and morphologic changes occurring after a standardized sciatic nerve crush injury with a nonserrated clamp. Functional recovery was evaluated using the sciatic functional index, the static sciatic index, the extensor postural thrust, the withdrawal reflex latency, and ankle kinematics. In addition, histomorphometric analysis was carried out on regenerated nerve fibers by means of the 2D-disector method. Based on the results of the EPT and of some of the ankle locomotor kinematic parameters analyzed, the hypothesis that N1E-115 cells may enhance nerve regeneration is partially supported although histomorphometry disclosed no significant difference in nerve fiber regeneration between the different experimental groups. Therefore, results suggest that enrichment of equine type III collagen membrane with the N1E-115 cellular system in the rat sciatic nerve crush model may support recovery, at least in terms of motor function. The discrepancy between functional and morphological results also suggests that the combined use of functional and morphological analysis should be recommended for an overall assessment of recovery in nerve regeneration studies.

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Section: 2mentioning

confidence: 99%

Neural cell transplantation effects on sciatic nerve regeneration after a standardized crush injury in the rat

et al. 2008

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“…1A). A major regulator of this neuronal differentiation process is p27 [16][17][18][19][20]25]. To test the effects of ectopic Bach1 and Bach2, we transfected logarithmically growing N1E-115 cells with plasmids harboring FLAG-tagged full-length Bach1 or Bach2 cDNA.…”

Section: Bach1 and Bach2 Negatively Regulate Proliferation Of N1e-115mentioning

confidence: 99%

“…N1E-115 neuronal precursor cells proliferate in normal medium but undergo neuronal differentiation in response to DMSO, cAMP, or serum withdrawal. Upon induction of differentiation, proliferation of N1E-115 cells ceases, and extensive neurite outgrowth is observed [16][17][18][19]. During N1E-115 cell differentiation, cdk2 protein expression decreases and loss of cdk4 activity is caused by strong association with the cdk inhibitor p27.…”

Section: Introductionmentioning

confidence: 99%

Bach2 is involved in neuronal differentiation of N1E-115 neuroblastoma cells

Shim

Rosner

Freilinger

et al. 2006

Experimental Cell Research

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“…Induction of tumor cell differentiation is already used clinically in the treatment of human leukemias with retinoic acids. Therefore, differentiation of malignant cells could be achieved by antisense ODNs which down-regulate differentiation-inhibiting genes such as c-jun or c-myc [7, 68,106]. Since solid tumors depend on the growth of blood vessels, antisense ODNs can be used to inhibit angiogenetic factors such as basic fibroblast growth factor (bFGF) or vascular endothelial growth factor (VEGF) in order to inhibit tumor angiogenesis [25,121].…”

Section: Potential Target Genes For Antisense Odns In Hematology and mentioning

confidence: 99%

Antisense strategies for the treatment of hematological malignancies and solid tumors

Kronenwett

Haas

1998

Annals of Hematology

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If malignant growth is considered the result of abnormal gene expression, it is reasonable to use antisense nucleic acids for the treatment of malignant diseases. Antisense oligonucleotides can specifically down-regulate gene expression, and a number of first-generation antisense compounds have entered human clinical trials. In this review, some aspects relevant for the development of antisense-based drugs, such as the selection of appropriate target sequences, cellular delivery, and design of a clinical study, are described, using bcr-abl-oncogene-directed antisense oligonucleotides as an example. In addition, potential target genes for antisense inhibition in hematology and oncology, including oncogenes and adhesion molecules, are summarized. Down-regulation of such adhesion molecules as members of the immunoglobulin superfamily and integrins may provide new modalities for mobilization of CD34+ hematopoietic stem cells into the peripheral blood. The review closes with an overview of ongoing clinical trials in the treatment of malignant diseases by antisense oligonucleotides.

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Growth inhibition of N1E-115 mouse neuroblastoma cells by C-myc or N-myc antisense oligodeoxynucleotides causes limited differentiation but is not coupled to neurite formation

Cited by 20 publications

References 20 publications

Neural cell transplantation effects on sciatic nerve regeneration after a standardized crush injury in the rat

Neural cell transplantation effects on sciatic nerve regeneration after a standardized crush injury in the rat

Bach2 is involved in neuronal differentiation of N1E-115 neuroblastoma cells

Antisense strategies for the treatment of hematological malignancies and solid tumors

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