Growth inhibition of human breast cancer cells and down-regulation of ODC1 and ADA genes by Nepeta binaloudensis

Afshar, Akbar Safipour; Nematpour, Fatemeh; Meshkani, Mahshid; Khafi, Arezosadat

doi:10.1016/j.bjp.2016.07.005

Cited by 17 publications

(15 citation statements)

References 32 publications

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“…The methanol extracts of N. scrophularioides [52] and N. praetervisa [53] also showed significant cytotoxicity in the brine shrimp lethality assay. Numerous investigations revealed cytotoxicity of Nepeta species against animal and human cancer cell lines [54][55][56]. Shakeri et al [57] showed cytotoxic effects of the essential oil of Nepeta ucrainica L. spp.…”

Section: Discussionmentioning

confidence: 99%

Differences in bioactivity of three endemic Nepeta species arising from main terpenoid and phenolic constituents

Živković¹,

Živković²,

Šiler³

et al. 2018

ARCH BIOL SCI BELGRA

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Methanol extracts of three endemic Nepeta species were analyzed for their main secondary metabolites, terpenes and phenolics, and further investigated for antioxidant capacity and embryonic toxicity in zebrafish. UHPLC/DAD/(±) HESI-MS/MS analysis showed that the dominant compound in N. rtanjensis was trans,cis-nepetalactone, the cis,trans isomer of this monoterpene lactone was dominant in N. sibirica, while nepetalactone was detected only in traces in N. nervosa. In all investigated species, rosmarinic acid was the dominant phenolic compound, while other identified phenolic acids (chlorogenic, neochlorogenic and caffeic) were present in considerably lower amounts. ABTS and DPPH assays showed that the methanol extracts of N. rtanjensis, N. sibirica and especially N. nervosa possessed strong antioxidant activities, with the FRAP assay revealing high ferric-reducing abilities for all three tested species. Such a strong antioxidant potential, especially as manifested in the DPPH and FRAP assays, can be attributed to phenolic acids, and in the first place to rosmarinic acid. Increased lethality of zebrafish embryos in any of the treatments was not observed, but several toxic effects on embryonic development were recorded, such as pericardial and yolk sac edema. As in other Nepeta species, the three studied endemic species possessed a great potential for food conservation or as medicinal supplements if applied in optimized concentrations; however, alternative sources of plant material (e.g. field cultivation) should be established bearing in mind their vulnerability in nature.

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Section: Discussionmentioning

confidence: 99%

Differences in bioactivity of three endemic Nepeta species arising from main terpenoid and phenolic constituents

Živković¹,

Živković²,

Šiler³

et al. 2018

ARCH BIOL SCI BELGRA

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show abstract

“…30 Polyamine putrescine is a product of ornithine, a reaction catalyzed by ornithine decarboxylase (ODC1), which is detectable in breast cancer cells and other cancer types. 31,32 Polyamines are essential for normal cell growth and development, and elevated levels have been associated with several different types of cancer. 33 The ornithine levels in primary breast cancer patients were lower than those in healthy controls, perhaps due to conversion to polyamine.…”

Section: Discussionmentioning

confidence: 99%

A plasma metabolite panel as biomarkers for early primary breast cancer detection

Yuan

Schafferer

Tang

et al. 2019

Intl Journal of Cancer

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In recent years, metabolites have attracted substantial attention as promising novel biomarkers of various diseases. However, breast cancer plasma metabolite studies are still in their infancy. Here, we investigated the potential of metabolites to serve as minimally invasive, early detection markers of primary breast cancer. We profiled metabolites extracted from the plasma of primary breast cancer patients and healthy controls using tandem mass spectrometry (UHPLC‐MS/MS and FIA‐MS/MS). Two metabolites were found to be upregulated, while 16 metabolites were downregulated in primary breast cancer patients compared to healthy controls in both the training and validation cohorts. A panel of seven metabolites was selected by LASSO regression analysis. This panel could differentiate primary breast cancer patients from healthy controls, with an AUC of 0.87 (95% CI: 0.81 ~ 0.92) in the training cohort and an AUC of 0.80 (95% CI: 0.71 ~ 0.87) in the validation cohort. These significantly differentiated metabolites are mainly involved in the amino acid metabolism and breast cancer cell growth pathways. In conclusion, using a metabolomics approach, we identified metabolites that have potential value for development of a multimarker blood‐based test to complement and improve early breast cancer detection. The panel identified herein might be part of a prescreening tool, especially for younger women or for closely observing women with certain risks, to facilitate decision making regarding which individuals should undergo further diagnostic tests. In the future, the combination of metabolites and other blood‐based molecular marker sets, such as DNA methylation, microRNA, and cell‐free DNA mutation markers, will be an attractive option.

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“…When compared to the control group, it was seen that ornithine decarboxylase 1 and adenosine deaminase messenger RNA expression were reduced 4.9‐fold and 3.5‐fold in the MCF‐7 cell line and 3.6‐fold and 2.6‐fold in the MDA‐MB‐231 cell line at 60 μg/ml of n ‐hexane extract concentration of N. binaloudensis . In conclusion, the study has shown that the n ‐hexane extract of N. binaloudensis displays an inhibitory action on the gene expression of ornithine decarboxylase 1 and adenosine deaminase in the breast cancer cell line (Afshar et al, ).…”

Section: Biological Activity Studies On Nepeta L Genusmentioning

confidence: 99%

“…Cytotoxic activity researches have shown that three Nepeta species, namely, N. sintenisii , N. ucrainica ssp. kopetdaghensis , and N. gloeocephala , displayed cytotoxic effect (Afshar et al, ; Shakeri et al, , ). N. meyeri and N. nuda caused genotoxic effects (Bozari et al, ; Kekeç et al, ).…”

Section: Biological Activity Studies On Nepeta L Genusmentioning

confidence: 99%

“…Several species are known for their medicinal properties and are used in folk medicine for their diuretic, diaphoretic, antitussive, antispasmodic, antiasthmatic, febrifuge, emmenagogue, and sedative activities (Formisano, Rigano, & Senatore, ; Nestorović et al, ; Shakeri et al, ). Nepeta species have been assessed mainly for their potential antitumor, anti‐inflammatory, and antimicrobial effects (Afshar, Nematpour, Meshkani, & Khafi, ; J. Hussain, Rehman, Hussain, Ali, & Al‐Harrasi, ; Kumar, Mathela, Tewari, & Singh, ; Nestorović et al, ; Nostro, Cannatelli, Crisafi, & Alonzo, ). The leaves of Nepeta species are also prepared and consumed as herbal tea, and the essential oils are used as perfumes or fragrances and for food flavoring (Khajeh, Yamini, & Shariati, ).…”

Section: Introductionmentioning

confidence: 99%

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Pharmacological and chemical features of Nepeta L. genus: Its importance as a therapeutic agent

Süntar

Nabavi

Barreca

et al. 2017

Phytotherapy Research

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Medicinal plants have always had great value for the human population due to their valuable constituents and potential bioactivities. The objective of this review is to present an updated overview of an important medicinal plant genus Nepeta L., from the family Lamiaceae, revealing its traditional utilization, biological activity, phytoconstituents, and mechanisms of action. For this purpose, a literature survey was carried out by using SciFinder, ScienceDirect, Scopus, PubMed, and Web of Science followed by a revision of the bibliographies of the related articles. We have described and analyzed the role of plants in drug discovery and the importance of Nepeta species. Information on the utilization purposes of Nepeta species in folk medicine has been emphasized, and scientific studies on the biological effects and secondary metabolites are addressed. Nepeta species are characterized by terpenoid-type compounds and phenolic constituents, which exert several activities such as an antimicrobial, repellent against major pathogen vector mosquitoes, insecticide, larvicide against Anopheles stephensi, cytotoxic anticarcinogen, antioxidant, anticonvulsant, analgesic, anti-inflammatory agent, and antidepressant, revealing its importance in medicinal and agricultural fields. On the basis of numerous studies, the Nepeta genus demonstrates remarkable therapeutic effects against various diseases. However, clinical studies are warranted to confirm preclinical findings.

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Growth inhibition of human breast cancer cells and down-regulation of ODC1 and ADA genes by Nepeta binaloudensis

Cited by 17 publications

References 32 publications

Differences in bioactivity of three endemic Nepeta species arising from main terpenoid and phenolic constituents

Differences in bioactivity of three endemic Nepeta species arising from main terpenoid and phenolic constituents

A plasma metabolite panel as biomarkers for early primary breast cancer detection

Pharmacological and chemical features of Nepeta L. genus: Its importance as a therapeutic agent

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