Growth inhibition and morphological alterations of Aspergillus niger by essential oils from Thymus eriocalyx and Thymus x-porlock

Rasooli, Iraj; Rezaei, Mohammad; Allameh, Abdolamir

doi:10.1016/j.foodcont.2004.12.002

Cited by 197 publications

(110 citation statements)

References 16 publications

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“…Changes in plasma membranes and mitochondria were also reported by Rasooli et al [29] who investigated the action of the essential oil of two species of Thymus on A. niger. TEM observations by de Billerbeck et al [30], carried out to determine the ultrastructural modifications of A. niger hyphae after treatment with Cymbopogon nardus (L.) essential oil, revealed reduced diameter and thinning of the hyphal walls.…”

Section: Discussionsupporting

confidence: 64%

In Vitro Inhibition of Growth and Aflatoxin B1 Production of Aspergillus Flavus Strain (ATCC 16872) by Various Medicinal Plant Essential Oils

Deabes¹,

El-Soud²,

El-Kassem³

2011

Macedonian Journal of Medical Sciences

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The hazardous nature of aflatoxins to human and animals necessitate the need for establishment of control measures. The objective of this study was to evaluate the inhibition of growth and aflatoxin production of Aspergillus flavus strain (ATCC 16872) by various essential oils in Yeast Extract Sucrose (YES) growth media at 25°C. Essential oils of basil, fennel, coriander, caraway, peppermint and rosemary were tested for their effects on mycelial growth and aflatoxin production. Aflatoxin B1 production was determined by high performance liquid chromatography (HPLC). The findings of this study revealed the antifungal efficacy of the all tested essential oils. The extent of inhibition of fungal growth and aflatoxin production was dependent on the type and concentration of essential oils used. The complete inhibition of Aspergillus flavus growth was observed at 1000 ppm concentrations of essential oils of basil, coriander, caraway and rosemary. While, essential oils of basil and coriander showed marked inhibition of aflatoxin B1 produced by Aspergillus flavus at all concentrations tested 500,750 and 1000 ppm.

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Section: Discussionsupporting

confidence: 64%

In Vitro Inhibition of Growth and Aflatoxin B1 Production of Aspergillus Flavus Strain (ATCC 16872) by Various Medicinal Plant Essential Oils

Deabes¹,

El-Soud²,

El-Kassem³

2011

Macedonian Journal of Medical Sciences

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“…In an ultra-structural study, Rasooli et al (2006) showed that the essential oils of Thymus eriocalyx (Ronniger) Jalas and T. x-porlock provoke severe damage to the walls, membranes, and cell organelles of Aspergillus niger spores, when in direct contact, causing morphologic alterations in the hyphae, rupture of the plasma membrane, and mitochondrial destruction.…”

Section: Introductionmentioning

confidence: 99%

Antibacterial activity of essential oils on Xanthomonas vesicatoria and control of bacterial spot in tomato

Lucas

Alves

Pereira

et al. 2012

Pesq. agropec. bras.

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-The objective of this work was to evaluate the effects of plant essential oils (EOs) on the growth of Xanthomonas vesicatoria, on bacterial morphology and ultrastructure, and on the severity of tomato bacterial spot. EOs from citronella, clove, cinnamon, lemongrass, eucalyptus, thyme, and tea tree were evaluated in vitro at concentrations of 0.1, 1.0, 10, and 100% in 1.0% powdered milk. The effect of EOs, at 0.1%, on the severity of tomato bacterial spot was evaluated in tomato seedlings under greenhouse conditions. The effects of citronella, lemongrass, clove, and tea tree EOs, at 0.1%, on X. vesicatoria cells were evaluated by transmission electron microscopy. All EOs showed direct toxic effect on the bacteria at a 10%-concentration in vitro. Under greenhouse conditions, the EOs of clove, citronella, tea tree, and lemongrass reduced disease severity. EOs of clove and tea tree, and streptomycin sulfate promoted loss of electron-dense material and alterations in the cytoplasm, whereas EO of tea tree promoted cytoplasm vacuolation, and those of citronella, lemongrass, clove, and tea tree caused damage to the bacterial cell wall. The EOs at a concentration of 0.1% reduce the severity of the disease.Index terms: alternative control of plant disease, cell ultrastructure, pathogenic bacteria. Atividade antibacteriana de óleos essenciais sobre Xanthomonas vesicatoria e controle da mancha-bacteriana do tomateiroResumo -O objetivo deste trabalho foi avaliar o efeito de óleos essenciais (OEs) no crescimento de Xanthomonas vesicatoria, na morfologia e na ultraestrutura bacteriana, e na severidade da mancha-bacteriana do tomateiro. OEs de citronela, cravo-da-índia, canela, capim-limão, eucalipto, tomilho e árvore-de-chá foram avaliados in vitro nas concentrações de 0,1, 1,0, 10 e 100% em leite em pó a 1,0%. O efeito dos OEs, a 0,1%, na severidade da mancha-bacteriana do tomateiro foram avaliados em plantas de tomateiro em casa de vegetação. Os efeitos dos óleos de citronela, capim-limão, cravo-da-índia e árvore-de-chá, a 0,1%, nas células de X. vesicatoria foram avaliados por meio de microscopia eletrônica de transmissão. Todos os OEs apresentaram efeito tóxico direto sobre as bactérias na concentração de 10% in vitro. Em casa de vegetação, os OEs de cravo-da-índia, citronela, árvore-de-chá e capim-limão reduziram a severidade da doença. Os OEs de cravo-da-índia e árvore-de-chá, e o sulfato de estreptomicina promoveram perda de material eletro-denso e alterações no citoplasma, enquanto o OE de árvore-de-chá promoveu vacuolização do citoplasma, e os de citronela, capim-limão, cravo-da-índia e árvore-de-chá causaram danos à parede celular bacteriana. Os OEs, na concentração de 0,1%, reduzem a severidade da doença.Termos para indexação: controle alternativo de doenças de plantas, ultraestrutura celular, bactéria fitopatogênica.

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“…On the other hand, Kumar et al (2008) and RazzaghiAbyaneh et al (2009) reported that Euclayptus citriodora, Euclayptus camaldulensis and T. vulgaris significantly inhibited mycelial growth and aflatoxins production by the toxigenic fungi. In addition, many species and herbs such as basil, cinnamon, marigold, arak and spearmint (Ahmed et al, 2008;Soliman and Badeaa, 2002), thyme (Rasooli et al, 2006;Pinto et al, 2006), cassia and sweat basil (Atanda et al, 2007) have been reported to inhibit toxigenic and food borne moulds. In the present study, the effect of five kinds of plant extracts including Euclayptus globolus, Olea europaea, Salvadora persica, Thymus vulgaris and Ziziphus spina-christi on growth and aflatoxin B 1 production of A. flavus are evaluated.…”

Section: Introductionmentioning

confidence: 99%

Antifungal and antiaflatoxigenic activities of some plant extracts

Al-Rahmah¹,

Mostafa²,

Abdel-Megeed³

2011

Afr. J. Microbiol. Res.

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The efficiency of five methanolic plant extracts obtained from Euclayptus globolus, Olea europaea, Salvadora persica, Thymus vulgaris and Ziziphus spina-christi were investigated for their inhibitory effect on the growth and aflatoxin B 1 production of the toxigenic Aspergillus flavus strain isolated from wheat seeds. Extract of T. vulgaris showed the highest antifungal and antiaflatoxigenic activities as aflatoxin B 1 production was absolutely arrested at 5 mg/ml, while the mycelial growth of A. flavus was completely inhibited at 15 mg/ml. E. globolus and O. europaea extracts showed a moderate antifungal activities and exhibited a significant antiaflatoxigenic efficiency as they inhibited aflatoxin B 1 production at 15 mg/ml. On the other hand, S. persica showed weak antifungal activities while no effect was detected with Z. spina-christi extract. The analysis of plant extracts by GC/MS revealed that T. vulgaris extract was mainly composed by thymol (38.73%), carvacrol (19.31%) and ρ-cimene (10.13%), O. europaea extract was composed by anthracenedione (20.63%), terbutaline (13.96%) and propiolic acid (12.02%) while eucalyptol (30.62%), globulol (18.94%) and silane (9.13%) were the principal components of E. globolus extract. These extracts of T. vulgaris, E. globolus and O. europaea which are effective and environmentally safe are promising for protecting wheat seeds against aflatoxigenic strain of A. flavus.

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Growth inhibition and morphological alterations of Aspergillus niger by essential oils from Thymus eriocalyx and Thymus x-porlock

Cited by 197 publications

References 16 publications

<i>In Vitro</i> Inhibition of Growth and Aflatoxin B1 Production of <i>Aspergillus Flavus</i> Strain (ATCC 16872) by Various Medicinal Plant Essential Oils

<i>In Vitro</i> Inhibition of Growth and Aflatoxin B1 Production of <i>Aspergillus Flavus</i> Strain (ATCC 16872) by Various Medicinal Plant Essential Oils

Antibacterial activity of essential oils on Xanthomonas vesicatoria and control of bacterial spot in tomato

Antifungal and antiaflatoxigenic activities of some plant extracts

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