Growth Inhibition and Induction of Apoptosis in NB4 Promyelocytic Leukemia Cells by Trypsin Inhibitor from Sweet Potato Storage Roots

Huang, Guan-Jhong; Sheu, Ming‐Jyh; Chen, Hsien-Jung; Chang, Yuan‐Shiun; Lin, Yi-Chun

doi:10.1021/jf063008m

Cited by 34 publications

(27 citation statements)

References 31 publications

(37 reference statements)

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“…The anti-proliferative effect and the mechanism of a 22 kDa trypsin inhibitor (TI) protein from sweet potato (Ipomoea batatas (L.)) storage roots on NB4 promyelocytic leukemia cells was reported by Huang et al (2007). TI arrested cell cycle at the G1 phase as determined by flow cytometry analysis and apoptosis as shown by DNA laddering.…”

Section: Roles Of Plant Protease Inhibitors In Health and Disease Conmentioning

confidence: 95%

Plant Protease Inhibitors in Therapeutics-Focus on Cancer Therapy

2016

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Plants are known to have many secondary metabolites and phytochemical compounds which are highly explored at biochemical and molecular genetics level and exploited enormously in the human health care sector. However, there are other less explored small molecular weight proteins, which inhibit proteases/proteinases. Plants are good sources of protease inhibitors (PIs) which protect them against diseases, insects, pests, and herbivores. In the past, proteinaceous PIs were considered primarily as protein-degrading enzymes. Nevertheless, this view has significantly changed and PIs are now treated as very important signaling molecules in many biological activities such as inflammation, apoptosis, blood clotting and hormone processing. In recent years, PIs have been examined extensively as therapeutic agents, primarily to deal with various human cancers. Interestingly, many plant-based PIs are also found to be effective against cardiovascular diseases, osteoporosis, inflammatory diseases and neurological disorders. Several plant PIs are under further evaluation in in vitro clinical trials. Among all types of PIs, Bowman-Birk inhibitors (BBI) have been studied extensively in the treatment of many diseases, especially in the field of cancer prevention. So far, crops such as beans, potatoes, barley, squash, millet, wheat, buckwheat, groundnut, chickpea, pigeonpea, corn, and pineapple have been identified as good sources of PIs. The PI content of such foods has a significant influence on human health disorders, particularly in the regions where people mostly depend on these kind of foods. These natural PIs vary in concentration, protease specificity, heat stability, and sometimes several PIs may be present in the same species or tissue. However, it is important to carry out individual studies to identify the potential effects of each PI on human health. PIs in plants make them incredible sources to determine novel PIs with specific pharmacological and therapeutic effects due to their peculiarity and superabundance.

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Section: Roles Of Plant Protease Inhibitors In Health and Disease Conmentioning

confidence: 95%

Plant Protease Inhibitors in Therapeutics-Focus on Cancer Therapy

2016

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“…It contains plenty of vitamin B complex, vitamin C, b-carotenoids, multiple minerals and high potassium and calcium (Hattori et al, 1985). Several medicative effects of sweet potato trypsin inhibitor, which is the major storage root proteins, have been reported, including antioxidant activity (Hou et al, 2001, Huang et al, 2007a, 2007b, inhibition of angiotensin converting enzyme activity Huang et al, 2006), and growth inhibition and induction of apoptosis in NB4 promyelocytic leukemia cells (Huang et al, 2007c).…”

Section: Introductionmentioning

confidence: 99%

Expression of sweet potato cysteine protease SPCP2 altered developmental characteristics and stress responses in transgenic Arabidopsis plants

Chen

Lin

et al. 2010

Journal of Plant Physiology

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“…Samples were mixed with sample buffer, namely 60 mmol L −1 Tris‐HCl buffer (pH 6.8) containing 2% SDS, 25% glycerol and 0.1% bromophenol blue with 2‐mercaptoethanol. Coomassie brilliant blue G‐250 was used for protein staining 22…”

Section: Methodsmentioning

confidence: 99%

Isolation and characterisation of invertase inhibitor from sweet potato storage roots

et al. 2008

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BACKGROUND: Plant invertases play important roles in sucrose metabolism. Cell wall invertase has been reported to participate in phloem loading and unloading. Soluble invertases are involved in hexose level regulation in mature tissues and in utilisation of stored sucrose within vacuoles. Invertase inhibitory proteins have been described as one of the possible components for invertase activity regulation in some plant species.

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Growth Inhibition and Induction of Apoptosis in NB4 Promyelocytic Leukemia Cells by Trypsin Inhibitor from Sweet Potato Storage Roots

Cited by 34 publications

References 31 publications

Plant Protease Inhibitors in Therapeutics-Focus on Cancer Therapy

Plant Protease Inhibitors in Therapeutics-Focus on Cancer Therapy

Expression of sweet potato cysteine protease SPCP2 altered developmental characteristics and stress responses in transgenic Arabidopsis plants

Isolation and characterisation of invertase inhibitor from sweet potato storage roots

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