Growth Inhibition and Apoptosis Induction by Vincetoxicum pumilum Decne. on HL-60 and K562 Leukemic Cell Lines

Najaran, Zahra Tayarani; Mousavi, Seyed Hadi; Rasoulinejad, Marzieh; Asili, Javad; Iranshahy, Milad; Emami, Seyed Ahmad

doi:10.17795/jjnpp-29370

Cited by 1 publication

(1 citation statement)

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“…The IC50 values Brazilian Archives of Biology and Technology. Vol.66: e23220358, 2023 www.scielo.br/babt calculated as 60.25 μg/mL in the methanol fraction of extract [40]. The results obtained in all these studies support our results.…”

Section: Discussionsupporting

confidence: 89%

Cytotoxic Potential of Rare Plant Salvia candidissima subsp. candidissima on Breast Cancer Cells

Ertürk

Onur

Aydin

et al. 2023

Braz. arch. biol. technol.

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Breast cancer is the leading cause of cancer-related deaths in women throughout the world. Research on natural anti-cancer products from plants has gained traction. Salvia L. species and their derivatives are rare in Turkey and have suggested for their potential anti-cancer effects. The aim of this study is to assess the potential cytotoxic/apoptotic activities of methanol extract of Salvia candidissima Vahl. subsp. candidissima (SCE) on MCF-7 and MDA-MB-231 breast cancer cells. A GCxGC-TOF/MS system and a dual stage commercial thermal desorption injector were used to determine the chemical components of SCE. MTT and ATP viability tests were used to investigate the anti-growth activity. The apoptosis-inducing effect was assessed using a fluorescence staining method. Caspase-cleaved keratin 18 (ccK18, M30-antigen) levels measured by M30-CytoDeath ELISA Kit. The results showed that SCE suppressed the survival of the MCF-7 and MDA-MB-231 breast cancer cells in a dose-dependent manner, based on the findings of both MTT and ATP cell viability tests and pyknotic cell nuclei were observed via fluorescent staining in both cell lines after 48 h of treatment. The treatment group had greater levels of caspase-cleaved keratin 18 in the MCF-7 cells than the untreated group. These results showed that SCE triggers apoptosis, causes cell death in MCF-7 and MDA-MB-231 cell lines. SCE may become promising therapeutic strategy in the treatment of breast cancer with further in vitro and in vivo studies. HIGHLIGHTS •Salvia species have been suggested for their potential anti-cancer effects.• Salvia candidissima Vahl. subsp. candidissima suppressed the survival of the breast cancer cells.• Breast cancer cells nuclei become pyknotic and fragmented after treatment. •The treatment group had greater levels of M30-antigen level in the MCF-7 cell line.

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Section: Discussionsupporting

confidence: 89%