Growth in Sterile Culture of Excised Leaves of Flowering Plants

Steeves, Taylor A.; Gabriel, H. Paul; Steeves, Margaret W.

doi:10.1126/science.126.3269.350

Cited by 31 publications

(7 citation statements)

References 4 publications

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“…The las, rax, and rev mutants have been previously described (Talbert et al, 1995;Greb et al, 2003;Müller et al, 2006). For in vitro leaf culture, P 8 to P 11 leaves were taken from plants grown on Murashige and Skoog medium in short-day conditions for 15 to 17 d. Detached leaves were cultured on Murashige and Skoog medium supplemented with 0.1 mg/L inositol acid and 0.5 mg/L folic acid under shortday conditions (Steeves et al, 1957;Wang et al, 2014b).…”

Section: Plant Materials and Treatment Conditionsmentioning

confidence: 99%

Cytokinin Signaling Activates WUSCHEL Expression during Axillary Meristem Initiation

et al. 2017

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The homeodomain transcription factor WUSCHEL (WUS) defines the shoot stem cell niche, but the mechanisms underlying the establishment of WUS expression remain unclear. Here, we show that cytokinin signaling precedes WUS expression in leaf axils and activates WUS expression de novo in the leaf axil to promote axillary meristem initiation. Furthermore, type-B Arabidopsis response regulator proteins, which are transcriptional activators in the cytokinin signaling pathway, directly bind to the WUS promoter and activate its expression. Finally, we show that cytokinin activation of WUS in the leaf axil correlates with increased histone acetylation and methylation markers associated with transcriptional activation, supporting the fact that WUS expression requires a permissive epigenetic environment to restrict it to highly defined meristematic tissues. Taken together, these findings explain how cytokinin regulates axillary meristem initiation and establish a mechanistic framework for the postembryonic establishment of the shoot stem cell niche.

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Section: Plant Materials and Treatment Conditionsmentioning

confidence: 99%

Cytokinin Signaling Activates WUSCHEL Expression during Axillary Meristem Initiation

et al. 2017

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“…Leaves between P 5 and P 11 were then detached from seedlings, laid flat on MS medium supplemented with 0.1 mg/L folic acid and 1 mL/L inositol (Steeves et al, 1957), and grown for a period of up to 30 d under the same conditions.…”

Section: Methods Plant Strains Growth Conditions and Iaa Treatment mentioning

confidence: 99%

The Stem Cell Niche in Leaf Axils Is Established by Auxin and Cytokinin in Arabidopsis

et al. 2014

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“…Many workers have shown by microsurgery that the longer a leaf primordium remains in contact with the shoot apex, the more complex will be its eventual morphology upon isolation. This is true both of angiosperms (Sussex 1952(Sussex , 1953Snow and Snow 1954;Steeves et al 1957;Cutter 1958;Pellegrini 1959;Smith 1984) and of ferns (Wardlaw 1949;Cutter 1954;Steeves 1961aSteeves , 1961bSteeves , 1962Steeves , 1966Kuehnert and Steeves 1962;Kuehnert 1967Kuehnert , 1969aKuehnert , 1969bKuehnert , 1969cHicks and Steeves 1969;Haight and Kuehnert 1971). By way of an explanation, Crotty (1955) proposed that the eventual morphology of a leaf primordium is a function of its "biological capital" of cells.…”

Section: Introductionmentioning

confidence: 92%

Does growth rate determine leaf form in Pisum sativum?

Gould¹,

Cutter²,

Young³

1989

Can. J. Bot.

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W. 1989. Does growth rate determine leaf form in Pisum sativum? Can. J. Bot. 67: 2590-2595. We have examined the long-standing hypothesis that leaves are morphologically more complex following prolonged proximity to the shoot apical meristem. Growth rates of the petiole and rachis of conventional and mutant pea leaves were compared for successive nodes of insertion in seedling plants. Leaves were longer at higher nodes, though the relative growth rate did not vary. Mature afila leaves were longer than those of conventional and tendril-less genotypes. The afila leaf alone exhibited a transient, highly significant rise in relative growth rate during the plastochron interval P,,,-P,,,.This rise occurred after the stage at which leaves of the different genotypes were anatomically distinguishable (stage P,-P,). Rates of vertical displacement of the leaf primordiurn from the shoot apical meristem did not differ significantly among genotypes. Our data suggest that the rate of leaf extension is one of the consequences, rather than a cause, of leaf morphology. GOULD, K. S., CUTTER, E. G., et YOUNG, J. P. W. 1989. Does growth rate determine leaf form in Pisun1 sativum? Can.J. Bot. 67 : 2590-2595. Nous avons reconsidtrt l'hypothese qui existe depuis dtjB quelque temps, voulant que les feuilles soient morphologiquement plus complexes lorsqu'elles demeurent longtemps B proximitt apical de la tige. Les taux de croissance du pttiole et du rachis de feuilles conventionnelles et mutantes du pois ont Ct C comparts pour des neouds successifs d'insertion chez des semis. Les feuilles ttaient plus grandes aux noeuds suptrieurs quoique le taux relatif de croissance n'a pas varit. Les feuilles afiles complbtement difftrencites ttaient plus longues que celles des ginotypes conventionnels et sans vrilles. Seule la feuille afile a rnontrt une augmentation passagtre trts significative dans le taux relatif de croissance durant l'intemalle plastochronique P,., -P,,,. Cette augmentation est survenue aprbs le stade oh les feuilles de genotypes difftrents sont anatomiquement distinctes (stade P,-P,). Les taux de dkplacement vertical du primordiurn foliaire du mtristbme apical de la tige n'ont pas varit de manitre significative entre les gtnotypes. Nos donnees suggbrent que le taux d'expansion foliaire est une des constquences plut6t que la cause de la morphologie foliaire.[Traduit par la revue]

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Growth in Sterile Culture of Excised Leaves of Flowering Plants

Cited by 31 publications

References 4 publications

Cytokinin Signaling Activates WUSCHEL Expression during Axillary Meristem Initiation

Cytokinin Signaling Activates WUSCHEL Expression during Axillary Meristem Initiation

The Stem Cell Niche in Leaf Axils Is Established by Auxin and Cytokinin in Arabidopsis

Does growth rate determine leaf form in Pisum sativum?

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