Growth improvement of Lolium multiflorum Lam. induced by seed inoculation with fungus suspension of Xerocomus badius and Serendipita indica

Abstract

In this study, a pot experiment was carried out in greenhouse to investigate the potentials of Xerocomus badius and Serendipita indica to penetrate and colonize roots of ryegrass (Lolium multiflorum Lam.) and to induce beneficial effects on seed germination and seedling growth. The results showed that X. badius and S. indica successfully colonized in the root system of L. multiflorum seedlings and the root colonization rate was 72.65% and 88.42%, respectively. By microscopy, the hyphae, chlamydospores and spores produced by S. indica were observed in roots cortex of L. multiflorum seedlings. In comparison with the non-inoculated seedlings, seedlings inoculated with X. badius and S. indica showed significant increase in growth parameters with plant height, basal diameter, biomass accumulation, relative growth rate, leaf relative water content and chlorophyll content. Also, we found that seedlings inoculated with S. indica exhibited a greater growth-promotion as compared with X. badius-inoculated seedlings. No significant influence of the two fungus application has been observed with respect to seed germination. It suggested that well establishments of mutualistic symbiosis between L. multiflorum and X. badius or S. indica were not so essential to seed germination but contributed highly to the survival and growth of the seedlings.

Plant Growth-Promoting Fungi (PGPF) Instigate Plant Growth and Induce Disease Resistance in Capsicum annuum L. upon Infection with Colletotrichum capsici (Syd.) Butler & Bisby

“…The current results are in accordance with the findings of other researchers, wherein seeds treated with a conidial suspension of PGPF resulted in enhanced disease protection against the invading pathogens in many crop plants [9,11,12,54]. The induction of resistance offered by PGPF is ascribed to the ability of PGP traits that, in turn, alter the metabolic pathways that help the host plants to defend against the invading pathogens [14,50].…”

“…The obtained results were in corroboration with other studies, wherein antagonistic studies were regarded as the primary criteria to select the positive isolates for their evaluation against disease resistance during host-pathogen interaction in many crop plants [10,49,50]. Further, the antagonistic nature exhibited by PGPF is attributed to hyper parasitism, antibiosis (synthesis of antibiotics), production of lytic enzymes or competition for space and nutrients, which directly help in the management of invading pathogens [12,15,[49][50][51]. The qualitative analysis on the production of PGP traits confirmed that all the selected antagonistic isolates were positive for IAA, except NBP-44 and NBP-65, which were found negative for all the other traits evaluated.…”

Stress tolerance of Xerocomus badius and its promotion effect on seed germination and seedling growth of annual ryegrass under salt and drought stresses

“…Based on the findings from the tolerance test of X. badius and the verified mutualistic symbiosis between L. multiflorum Lam. and X. badius driven by seed inoculation (Liu et al 2019 ), the effect of seed priming with spore suspensions of X. badius on seed germination and seedling growth of L. multiflorum Lam. were investigated under different salt and drought conditions.…”

“…Based on the findings from the tolerance test with X. badius and the verified mutualistic symbiosis between Lolium multiflorum Lam. and X. badius driven by seed inoculation (Liu et al 2019), we propose that X. badius is expected to enhance stress tolerance of L. multiflorum Lam. under drought and salt stresses.…”

“…Fungus maintenance, incubation, inoculation, and seeds pretreatment followed the methods of Liu et al. (Liu et al 2019 ).…”