Growth, immune responses and intestinal morphology of rainbow trout (Oncorhynchus mykiss) supplemented with commercial probiotics

Ramos, M.A.; Gonçalves, José F.; Batista, Sónia; Costas, Benjamín; Pires, M.A.; Rema, Paulo; Ozório, R.O.A.

doi:10.1016/j.fsi.2015.04.001

Cited by 61 publications

(40 citation statements)

References 57 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Higher dosages may enhance changes in the microbiota and/or immune responses (Li et al, 2012), consequently increasing the energy expenditure, at expenses of growth. Other recent studies on bacterial consortia in rainbow trout juveniles observed an improvement on growth rate and FCR at 1.5 g/kg dietary probiotic supplementation, but not at 3 g/kg (Ramos et al, 2015) and at 1 g/kg but not at and 5 g/kg (Giannenas et al, 2015).…”

Section: Discussionmentioning

confidence: 72%

“…Fish from the same tank were pooled and homogenized. Dry matter (DM) content was determined in fresh samples of diets, faeces and carcass, whereas the proximate composition analyses was carried out in freeze-dried material using standard procedures as described in Ramos et al (2015).…”

Section: Sampling Proceduresmentioning

confidence: 99%

“…The proceedings were done in duplicate per fish. The alternative complement pathway (ACH50), lysozyme and peroxidase activities were determined in plasma (n = 12/ treatment) by references methodologies as described in Ramos et al (2015). The respiratory burst activity and the production of nitric oxide (NO) was evaluated on HK leucocytes.…”

Section: Sampling Proceduresmentioning

confidence: 99%

“…The isolation and culture of HK leucocytes were done aseptically. The cells were isolated, adhered to plates and maintained to conduct respiratory burst and NO assays, as described in Ramos et al (2015), after 24 and 72 h of incubation at 25°C, respectively. Respiratory burst activity of HK leucocytes was based on the reduction of ferricytochrome C method for the detection of extracellular O 2 -production.…”

Section: Sampling Proceduresmentioning

confidence: 99%

“…Some experiments report the stimulation of systemic innate defences as plasma lysozyme, complement or respiratory burst by probiotic microorganisms (Balcazar et al, 2007) and their implication in the oxidative stress response (Castex et al, 2009;Ozório et al, 2016). As result, animals become more resistant to infectious diseases (Castex et al, 2009) and/or present improved feed and growth performances (Pirarat et al, 2011;Ramos et al, 2015). The properties and effects described are not all displayed by a single microorganism, explaining why microbial consortia can be more effective (Verschuere et al, 2000).…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Dietary probiotic supplementation improves growth and the intestinal morphology of Nile tilapia

Ramos¹,

Batista²,

Pires

et al. 2017

Animal

Self Cite

View full text Add to dashboard Cite

Probiotic administration can be a nutritional strategy to improve the immune response and growth performance of fish. The current study aimed to evaluate the effects of a probiotic blend (Bacillus sp., Pediococcus sp., Enterococcus sp., Lactobacillus sp.) as a dietary supplement on growth performance, feed utilization, innate immune and oxidative stress responses and intestinal morphology in juvenile Nile tilapia (Oreochromis niloticus). The probiotic was incorporated into a basal diet at three concentrations: 0 g/kg (A 0 : control), 3 g/kg (A 1 : 1.0 × 10 6 colony forming unit (CFU)/g) and 6 g/kg (A 2 : 2.3 × 10 6 CFU/g diet). After 8 weeks of probiotic feeding, weight and specific growth rate where significantly higher in fish-fed A 1 diet than in fish-fed A 0 . Alternative complement in plasma was significantly enhanced in fish-fed A 2 when compared with A 0 . The hepatic antioxidant indicators were not affected by probiotic supplementation. Villi height and goblet cell counts increased significantly in the intestine of fish-fed A 1 and A 2 diets compared with A 0 . The dietary probiotic supplementation was maintained until 20 weeks of feeding. Then the selected immune parameters, digestive enzymes and apparent digestibility of diets were studied. No effect of probiotic feeding was observed after that longer period supplementation. The dietary supplementation of mixed species probiotic may constitute a valuable nutritional approach towards a sustainable tilapia aquaculture. The improvement of the immune responses and intestinal morphology play an important role in increasing growth performance, nutrient absorption and disease resistance in fish, important outcomes in such a competitive and developing aquaculture sector.

show abstract

Section: Discussionmentioning

confidence: 72%

Section: Sampling Proceduresmentioning

confidence: 99%

Section: Sampling Proceduresmentioning

confidence: 99%

Section: Sampling Proceduresmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Dietary probiotic supplementation improves growth and the intestinal morphology of Nile tilapia

Ramos¹,

Batista²,

Pires

et al. 2017

Animal

Self Cite

View full text Add to dashboard Cite

show abstract

The influence of probiotics of different microbiological composition on histology of the gastrointestinal tract of juvenile Oncorhynchus mykiss

Nikiforov-Nikishin

Kochetkov

et al. 2021

Microscopy Res & Technique

View full text Add to dashboard Cite

This article presents results of the influence of three probiotic feed additive of various microbiological composition: Bacillus subtilis (VKPM B‐2335); B. subtilis (OZ‐2 VKPM‐11966) + Bacillus amyloliquefaciens (OZ‐3 VKPM‐11967); Lactobacillus acidophilus (VKPM B‐3235) on the growth and histology of the organs of the gastrointestinal tract of juvenile Oncorhynchus mykiss by morphometric parameters. These probiotic bacteria are the most commonly used in aquaculture. The effect of the probiotic feed additive led to the increase in fish growth and influenced different sections of the gastrointestinal tract. The biggest change was found in the mid intestine and the reliable difference compared with the control diet was obtained at the following parameters: lamina propria width, intraepithelial lymphocytes number of prismatic epithelium and goblet cells area. The changes in the pyloric appendages were less obvious but reported as playing an important functional role in digestion. The liver preserved normal functional structure in all series of the experiment except for the group with L. acidophilus, where hepatocyte small‐drop vacuolization was observed. That might be connected with the change of the digest activity resulting from a decrease in secretory activity of the intestinal exocrinocytes. The use of all probiotic feed additives led to a similar change in morphometric parameters in all groups, which suggests a decrease in the immune response.

show abstract

In Vitro and in Vivo Characterization of Inosine Monophosphate Delivered by a Nanoparticle in Rainbow Trout

et al. 2022

View full text Add to dashboard Cite

Rainbow Trout Oncorhynchus mykiss are a commonly farmed fish worldwide and, as such, are of economic importance in many countries. Inosine monophosphate (IMP) has been shown to have a positive effect on Rainbow Trout growth performance and overall health. The aim of the current study was to test whether a novel phytoglycogen nanoparticle (NP) was nontoxic and if it could enhance the positive effects of IMP on Rainbow Trout health at the cellular and whole-animal level. The NP used in this study is derived from sweet corn and can act as a carrier, delivering its payload to the cell. The NP was chemically modified to covalently bind IMP. The effects of four experimental groups were tested on Rainbow Trout at the in vitro and in vivo level: untreated control, NP alone, IMP alone, and IMP covalently conjugated to the NP (IMP-NP). The effects of the four experimental groups were measured at the cellular level using a Rainbow Trout intestinal epithelial cell line (RTgutGC). Cellular metabolism was significantly increased in RTgutGC when treated with IMP-NP for 24 and 48 h compared with NP or IMP alone. These treatments did not result in a compromised cell membrane, with the exception of minimal membrane integrity loss at 48 h with the higher doses of IMP-NP. During the 48-h time frame, enhanced metabolism did not result in enhanced cell proliferation. A pilot study was performed in Rainbow Trout to test the safety of the NP to fish. Over an 8-week feeding trial, no mortalities were observed. Additionally, intestinal villi density increased with IMP-NP and NP treatments, while villus height and width were unaffected. This study introduces the use of a phytoglycogen-based NP as a novel delivery system for IMP that is able to increase metabolism at the cellular level and is nontoxic in Rainbow Trout.

show abstract

Growth, immune responses and intestinal morphology of rainbow trout (Oncorhynchus mykiss) supplemented with commercial probiotics

Cited by 61 publications

References 57 publications

Dietary probiotic supplementation improves growth and the intestinal morphology of Nile tilapia

Dietary probiotic supplementation improves growth and the intestinal morphology of Nile tilapia

The influence of probiotics of different microbiological composition on histology of the gastrointestinal tract of juvenile Oncorhynchus mykiss

In Vitro and in Vivo Characterization of Inosine Monophosphate Delivered by a Nanoparticle in Rainbow Trout

Contact Info

Product

Resources

About