Growth hormone protects against ovarian granulosa cell apoptosis: Alleviation oxidative stress and enhancement mitochondrial function

Wang, Qianqian; Wu, Jingjing; Zhang, Yameng; Zhang, Junqiang; Xu, Wenjuan; Wu, Caiyun; Zhou, Ping

doi:10.1016/j.repbio.2021.100504

Cited by 19 publications

(14 citation statements)

References 42 publications

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“…Their growth, proliferation and division require abundant and stable mitochondria to supply appropriate amounts of energy [ 57 ]. During proliferation, granulosa cells experience a significant increase in ROS levels and mtDNA damage [ 58 ]. At the same time, granulosa cells exhibit reduced mitochondrial membrane potential and reduced expression levels of mitochondrial-related genes (Nd1, Cytb, Cox1 and ATPase6), ultimately resulting in a poor state of decreased viability and cell cycle arrest [ 59 ].…”

Section: Pathological Mechanisms Related With Os In Ovarian Agingmentioning

confidence: 99%

The role of oxidative stress in ovarian aging: a review

et al. 2022

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Ovarian aging refers to the process by which ovarian function declines until eventual failure. The pathogenesis of ovarian aging is complex and diverse; oxidative stress (OS) is considered to be a key factor. This review focuses on the fact that OS status accelerates the ovarian aging process by promoting apoptosis, inflammation, mitochondrial damage, telomere shortening and biomacromolecular damage. Current evidence suggests that aging, smoking, high-sugar diets, pressure, superovulation, chemotherapeutic agents and industrial pollutants can be factors that accelerate ovarian aging by exacerbating OS status. In addition, we review the role of nuclear factor E2-related factor 2 (Nrf2), Sirtuin (Sirt), mitogen-activated protein kinase (MAPK), protein kinase B (AKT), Forkhead box O (FoxO) and Klotho signaling pathways during the process of ovarian aging. We also explore the role of antioxidant therapies such as melatonin, vitamins, stem cell therapies, antioxidant monomers and Traditional Chinese Medicine (TCM), and investigate the roles of these supplements with respect to the reduction of OS and the improvement of ovarian function. This review provides a rationale for antioxidant therapy to improve ovarian aging.

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Section: Pathological Mechanisms Related With Os In Ovarian Agingmentioning

confidence: 99%

The role of oxidative stress in ovarian aging: a review

et al. 2022

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“…However, it is proved that the prolactin-like effect of GH can reduce estradiol synthesis catalyzed by follicle stimulating hormone (FSH) and increase progesterone level. This may also be due to that the regulatory effect of GH on the ovarian function depends on the stage of granulosa cells in menstrual cycle ( 20 ).…”

Section: The Direct Regulation Of Gh On Ovarian Functionmentioning

confidence: 99%

“…The outcome indicators of these patients when receiving assisted reproductive technology also improved accordingly. Besides, the content of cumulus granulosa cell mtDNA may also be related to oocyte quality and embryo implantation potential ( 20 ). GH can improve the number of retrieved oocytes, embryo implantation rate, clinical pregnancy rate, live birth rate and mtDNA content in cumulus granulosa cells of patients with normal ovarian reserve but low rate of high-quality embryos ( 29 ).…”

Section: Regulation Of Gh On Oocyte Qualitymentioning

confidence: 99%

“…Compared with the control group, POR patients who received GH treatment had significantly lower oxidative stress level in follicular fluid and granulosa cells, and significantly increased the number of high-quality embryos and clinical pregnancy rate. It was speculated that reducing oxidative stress might be one of the mechanisms of GH improving oocyte quality ( 20 ).…”

Section: Regulation Of Gh On Oocyte Qualitymentioning

confidence: 99%

“…Clinical study found that only increasing the dosage of gonadotropin in the COH process of patients with POR and DOR could not promote ovarian response. Some of the patients may be due to the lack of FSHR or functional mitochondria in granulosa cells ( 20 ). IGF-I is known to increase the expression of FSHR mRNA in granulosa cells to enhance the stimulating effects of FSH, which indicates that GH may promote ovarian response mainly through indirect regulation ( 31 ).…”

Section: Dosage Of Gh and Ovarian Responsementioning

confidence: 99%

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Regulation of ovarian function by growth hormone: Potential intervention of ovarian aging

et al. 2023

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Growth hormone (GH) is mainly secreted by eosinophils of anterior pituitary gland. GH plays an important role in regulating the growth and development of many tissues and cells, so it is used in the treatment of many diseases. In recent years, the regulation of GH on ovarian function has attracted much attention. GH has been applied in controlled ovarian hyperstimulation, particularly in the patients with advanced age, diminished ovarian reserve (DOR) and poor ovarian response (POR). GH can directly bind to the growth hormone receptor (GHR) on the ovary to promote the growth, maturation and ovulation of follicles, as well as to inhibit follicular atresia. GH so as to promote the occurrence of early follicles, enhance the sensitivity of follicles to gonadotropins, accelerate the maturation of oocyte nucleus, improve mitochondrial activity and the quality of oocytes through the insulin-like growth factor (IGF) system, which is an indirect regulation. The deep-seated effects of GH on human reproduction and ovarian aging need further basic research and clinical practice.

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Mature oocyte dysmorphisms may be associated with progesterone levels, mitochondrial DNA content, and vitality in luteal granulosa cells

Raad,

Tanios,

Serdarogullari

et al. 2024

J Assist Reprod Genet

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Purpose To identify whether follicular environment parameters are associated with mature oocyte quality, embryological and clinical outcomes. Methods This retrospective study examined 303 mature oocytes from 51 infertile women undergoing ICSI cycles between May 2018 and June 2021. Exclusion criteria consisted of advanced maternal age (> 36 years old), premature ovarian failure, obesity in women, or use of frozen gametes. Luteal granulosa cells (LGCs) were analyzed for mitochondrial DNA/genomic (g) DNA ratio and vitality. The relationships between hormone levels in the follicular fluid and oocyte features were assessed. Quantitative morphometric measurements of mature oocytes were assessed, and the association of LGC parameters and oocyte features on live birth rate after single embryo transfer was examined. Results Results indicated an inverse correlation between the mtDNA/gDNA ratio of LGCs and the size of polar body I (PBI). A 4.0% decrease in PBI size was observed with each one-unit increase in the ratio (p = 0.04). Furthermore, a 1% increase in LGC vitality was linked to a 1.3% decrease in fragmented PBI (p = 0.03), and a 1 ng/mL increase in progesterone levels was associated with a 0.1% rise in oocytes with small inclusions (p = 0.015). Associations were drawn among LGC characteristics, perivitelline space (PVS) debris, cytoplasmic inclusions, PBI integrity, and progesterone levels. Certain dysmorphisms in mature oocytes were associated with embryo morphokinetics; however, live birth rates were not associated with follicular parameters and oocyte quality characteristics. Conclusion Follicular markers may be associated with mature oocyte quality features.

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Growth hormone protects against ovarian granulosa cell apoptosis: Alleviation oxidative stress and enhancement mitochondrial function

Cited by 19 publications

References 42 publications

The role of oxidative stress in ovarian aging: a review

The role of oxidative stress in ovarian aging: a review

Regulation of ovarian function by growth hormone: Potential intervention of ovarian aging

Mature oocyte dysmorphisms may be associated with progesterone levels, mitochondrial DNA content, and vitality in luteal granulosa cells

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