Growth hormone in the presence of laminin modulates interaction of human thymic epithelial cells and thymocytes in vitro

Lins, Marvin Paulo; Vieira, Larissa Fernanda de Araújo; Rosa, Alfredo Aurélio Marinho; Smaniotto, Salete

doi:10.1186/s40659-016-0097-0

Cited by 13 publications

(6 citation statements)

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“…In total, 69 articles were found that refer to these cell lines, in turn cited by 2092 articles. Of the primary articles, 43 were published after the report by MacLeod et al and the most recent one was published only in late 2016 [41]. Of the fifteen most recent articles referring to the 1994 report, thirteen actually refer to it because they use the cell lines, all thirteen reporting research on thymic cells, without mentioning any knowledge of the misidentification of these cell lines.…”

Section: Resultsmentioning

confidence: 99%

The ghosts of HeLa: How cell line misidentification contaminates the scientific literature

2017

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While problems with cell line misidentification have been known for decades, an unknown number of published papers remains in circulation reporting on the wrong cells without warning or correction. Here we attempt to make a conservative estimate of this ‘contaminated’ literature. We found 32,755 articles reporting on research with misidentified cells, in turn cited by an estimated half a million other papers. The contamination of the literature is not decreasing over time and is anything but restricted to countries in the periphery of global science. The decades-old and often contentious attempts to stop misidentification of cell lines have proven to be insufficient. The contamination of the literature calls for a fair and reasonable notification system, warning users and readers to interpret these papers with appropriate care.

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Section: Resultsmentioning

confidence: 99%

The ghosts of HeLa: How cell line misidentification contaminates the scientific literature

2017

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“…Most likely, at least part of the GH effects upon the thymus is related to the modulation of cell adhesion and migration. Accordingly, in vitro treatment of murine and human TECs with GH resulted in increased deposition of fibronectin and laminin and their respective receptors, VLA-5, and VLA-6 ( 114 , 115 ). Also, thymocyte adhesion to GH-treated TECs is upregulated, and exposure of these cells to anti-GH antibodies or anti-ECM abolished the effect ( 116 ).…”

Section: Pleiotropic Effects Of Gh Igf-1 Ghrelin and Somatostatin Upo...mentioning

confidence: 99%

Intrathymic somatotropic circuitry: consequences upon thymus involution

et al. 2023

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Growth hormone (GH) is a classic pituitary-derived hormone crucial to body growth and metabolism. In the pituitary gland, GH production is stimulated by GH-releasing hormone and inhibited by somatostatin. GH secretion can also be induced by other peptides, such as ghrelin, which interacts with receptors present in somatotropic cells. It is well established that GH acts directly on target cells or indirectly by stimulating the production of insulin-like growth factors (IGFs), particularly IGF-1. Notably, such somatotropic circuitry is also involved in the development and function of immune cells and organs, including the thymus. Interestingly, GH, IGF-1, ghrelin, and somatostatin are expressed in the thymus in the lymphoid and microenvironmental compartments, where they stimulate the secretion of soluble factors and extracellular matrix molecules involved in the general process of intrathymic T-cell development. Clinical trials in which GH was used to treat immunocompromised patients successfully recovered thymic function. Additionally, there is evidence that the reduction in the function of the somatotropic axis is associated with age-related thymus atrophy. Treatment with GH, IGF-1 or ghrelin can restore thymopoiesis of old animals, thus in keeping with a clinical study showing that treatment with GH, associated with metformin and dehydroepiandrosterone, could induce thymus regeneration in healthy aged individuals. In conclusion, the molecules of the somatotrophic axis can be envisioned as potential therapeutic targets for thymus regeneration in age-related or pathological thymus involution.

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“…СТГ регулирует тимическое микроокружение, повышая секрецию и высвобождение цитокинов (прежде всего интерлейкина-1 и интерлейкина-6 [37]), хемокинов и тимулина [7,35,38,39,43,46], модулируя при этом адгезию и миграцию тимоцитов [32,36,37], а также выработку белков внеклеточного матрикса [36]; увеличивает отложение (депонирование) белков, способствующих миграции клеток, таких как ламинины и стромальный клеточный фактор-1 [44]. В эксперименте миграция тимоцитов, полученных от трансгенной по ГР мыши или от мыши, в тимус которой был инъецирован этот гормон, усиливается в сторону источников стромального клеточного фактора-1 и ламининов [44].…”

Section: тимус и гормоны соматотропной осиunclassified

Thymic gland aspects in childhood: morpho-functional reciprocal relationships betwen thymus, nervous and endocrine system, in particular, with the somatotrophic axis hormones

et al. 2023

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Thymic gland (thymus) represents a huge mystery for biology, medicine (primarily immunology), including pediatric issues. Complexity of the study is determined by the multiplicity of integral connections of thymus with other components of immune system, neuroendocrine, hematopoietic systems, connective tissue, different organs and cells which provide appropriate barrier function. Discerning the direct thymic function from this continuum, or determining specific role of molecular factors (neuropeptides, growth hormone, etc.) upon the immune physiology represents a problem which is not yet resolved. In this review article dedicated to the current state of the problem, we consider the morphological and functional relationships between thymus, neuroendocrine system and, in particular, with hormones of the somatotropic axis. These interactions may also manifest by clinical heterogeneity which may be associated with impaired morphogenesis (organogenesis) at a very early stage of embryogenesis; namely, under the influence of gene family that determine the fate of each segment of the embryo-Hox genes which control the expression of other, functionally interconnected genes. Previously, T lymphocytes produced by the thymus and brain neurons have been shown to express the same antigen (Thy antigen), which was considered a specific antigen of T lymphocytes. A common molecular language, mediated by the molecules of intercellular interaction, was revealed which is used for the signal exchange between the cells, tissues and organs regulating the three mentioned systems (nervous, endocrine and immune). The interest of pediatricians in this field is associated with definite concept of human ontogenesis, from birth to elderly age, with thymic gland playing the main role, since antenatal period to early childhood. The main line of reasoning in this research area is not only theoretical, but also important from practical point of view. Since any critical involution of the thymus is accompanied by reduced number of produced and exported cells, a hormone-based therapy may be an alternative strategy to restore the organ by increasing thymocyte proliferation, and exporting mature T cells to peripheral lymphoid organs. Great opportunities have been opened in clinical immunology due to development of effective epistemological methods, e.g., genetic knock-out, transgenic animal models with human stem cell transfer, transplantation of hematopoietic and immunopoietic cells in primary and secondary immunodeficiencies, immune cell malignancies, autoinflammatory diseases, and, finally, infections of the immune system.

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Growth hormone in the presence of laminin modulates interaction of human thymic epithelial cells and thymocytes in vitro

Cited by 13 publications

References 40 publications

The ghosts of HeLa: How cell line misidentification contaminates the scientific literature

The ghosts of HeLa: How cell line misidentification contaminates the scientific literature

Intrathymic somatotropic circuitry: consequences upon thymus involution

Thymic gland aspects in childhood: morpho-functional reciprocal relationships betwen thymus, nervous and endocrine system, in particular, with the somatotrophic axis hormones

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