Growth Hormone (GH) and Gonadotropin Subunit Gene Expression and Pituitary and Plasma Changes during Spermatogenesis and Oogenesis in Rainbow Trout (Oncorhynchus mykiss)

Gomez, Jean Marc; Weil, Claudine; Ollitrault, Martine; Bail, Pierre‐Yves Le; Breton, Bernard; Gac, Florence Le

doi:10.1006/gcen.1998.7222

Cited by 266 publications

(198 citation statements)

References 67 publications

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“…The presence of LH␤ transcript in immature fish pituitary was also reported in another late-maturing fish, the black carp (Mylopharyngodon piceus) (Gur et al, 2000). In salmonids, however, LH␤ was not expressed during gametogenesis, whereas its transcript was only detected just before ovulation/spermiation (Breton et al, 1998;Gomez et al, 1999;Slater et al, 1994;Swanson et al, 1991;Weil et al, 1995). Our results suggested that the expression pattern of Chinese sturgeon LH␤ might be similar to that observed in salmonids.…”

Section: Discussionsupporting

confidence: 81%

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Molecular characterization of Chinese sturgeon gonadotropins and cellular distribution in pituitaries of mature and immature individuals

Cao

Zhou

Zhang

et al. 2009

Molecular and Cellular Endocrinology

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Section: Discussionsupporting

confidence: 81%

“…In salmonids, similar expression status of FSH␤ was also observed. Generally, FSH␤ concentration is high during the early stages of gametogenesis, then decreases during vitellogensis and maturation, and rises again at pre-ovulation period (Breton et al, 1998;Gomez et al, 1999;Slater et al, 1994;Swanson et al, 1991;Weil et al, 1995).…”

Section: Discussionmentioning

confidence: 99%

Molecular characterization of Chinese sturgeon gonadotropins and cellular distribution in pituitaries of mature and immature individuals

Cao

Zhou

Zhang

et al. 2009

Molecular and Cellular Endocrinology

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“…The GSI increased from 3.5% to 7% over this period (Davidson et al 2014). Previous studies of females from winter-spawning or autumn-spawning strains of Rainbow Trout exposed to ambient photoperiod and temperature conditions showed either no change in plasma GH before ovulation (Sumpter et al 1991;Gomez et al 1999) or slightly greater GH concentrations during the second half of ovarian growth (Holloway et al 1999). The small or nonexistent changes in plasma GH in female Rainbow Trout going through vitellogenesis and approaching spawning is inconsistent with changes observed over the same developmental period in Atlantic Salmon Salmo salar (Björnsson et al 1994;Benedet et al 2010), suggesting differences in the roles of GH during reproduction among salmonids.…”

Section: Discussionmentioning

confidence: 99%

Changes in Sex Steroids, Growth Hormone, and Insulin‐Like Growth Factor‐I during Ovarian Development in Rainbow Trout Cultured in a Water Recirculating System with Continuous Light

et al. 2015

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Female Rainbow Trout Oncorhynchus mykiss were cultured within a freshwater recirculating aquaculture system under 24‐h constant lighting in 13°C water and fed every 6 h to near satiation. An opaque roof allowed surface light intensity to vary between <200 and about 1,500 lx. During months 14–26 posthatch we examined changes in plasma concentrations of testosterone (T), estradiol‐17β (E2), the maturation inducing steroid (MIS) 17α,20β‐dihydroxy‐4‐pregnen‐3‐one (17,20βP), growth hormone (GH), and insulin‐like growth factor‐I (IGF‐I). Oocyte diameter was variable at the start of the study, most averaging <1 mm; diameter increased to above 3.2 mm in the final 2 months, with migrating germinal vesicles indicating they were postvitellogenic. Some ovaries exhibited atresia, and no fish ovulated, suggesting some reproductive dysfunction. Testosterone and E2 began increasing between months 16 and 18, and although T continued to increase throughout the study, E2 changed little after month 20. The MIS 17,20βP, remained near or below detection. Plasma GH remained relatively unchanged although values trended slightly higher during the final 4 months, GH being significantly greater at months 22, 24 and 26 than at month 16. Plasma IGF‐I was higher at the first time point, month 14 posthatch than at months 20, 24, 25, and 26. In summary, gradual changes in growth and fillet quality attributes during gonadal development were accompanied by gradual changes in hormone levels, but no clear changes in hormones were associated with rapid changes in product quality traits observed around months 24–26.

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“…The high increase of 17,20β-P in salmonid during the spawning season coincides with a clear increase of Lh in plasma during spermiation (Gomez et al, 1999). Furthermore, during the complete testicular cycle, Lh or Fsh can stimulate 17,20β-P both in vivo and in vitro, but Lh stimulation is greater during the late testes development phases (Le Gac & Loir, 1988;Schulz & Blüm, 1990;Schulz et al, 1992;Planas et al, 1993;Planas & Swanson, 1995;Vizziano & Le Gac, 1998).…”

Section: Introductionmentioning

confidence: 99%

17,20β-P and cortisol are the main in vitro metabolites of 17-hydroxy-progesterone produced by spermiating testes of Micropogonias furnieri (Desmarest, 1823) (Perciformes: Sciaenidae)

Cantonnet¹,

Mateo²,

Alberro³

et al. 2015

Neotrop. ichthyol.

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The aim was to investigate the major C21 steroids produced by spermiating white croaker Micropogonias furnieri (Sciaenidae) in order to establish the potential mediator of gamete maturation in males of this species. The testes steroid production at the spawning season was identified incubating the 3 H-17-hydroxy-4-pregnene-3,20-dione precursor through thin layer chromatography, high pressure liquid chromatography, enzymatic oxydation, acetylation and immunochemistry analyses. 17,17, were the main metabolites produced. Contrary to what we expected, 17,20β,21-Trihydroxy-4-pregnen-3-one was not detected. Circulating levels of 17,20β-P were undetectable in immature testes and in those at the first spermatogenesis stages, while a clear increase was observed during the whole spermatogenesis and spermiation phases (from undetectable to 1047 pg mL -1 ).In vitro studies together with plasma detection suggest that 17,20β-P is a good steroid candidate involved in M. furnieri testes maturation. The role of cortisol during late phases of testes development needs further studies.El objetivo fue investigar cuales eran los esteroides C21 más importantes producidos por los testículos en espermiación de la corvina blanca Micropogonias furnieri (Sciaenidae) para poder identificar los potenciales mediadores de la maduración gamética de los machos de esta especie. Los esteroides producidos por los testículos en espermiación fueron identificados después de su incubación con el precursor 3 H-17-hidroxi-4-pregnene-3,20-diona a través de cromatografía de capa fina y cromatografía líquida de alta presión y posteriormente por oxidación enzimática, acetilación e inmunoquímica. Los principales metabolitos producidos por los testículos en espermiación fueron la 17,20β-Dihidroxi-4-pregnen-3-ona (17,20β-P) y la 11β,17,21-Trihidroxi-4-pregnene-3,20-diona (cortisol). Contrariamente a lo esperado, no se encontró el derivado tri-hidroxilado de la progesterona llamado 17,20β,21-Trihidroxi-4-pregnen-3-ona. Los niveles circulantes de 17,20β-P fueron no detectable en los testículos inmaduros y en aquellos en inicios de espermatogénesis, mientras que un aumento claro en las concentraciones circulantes fue detectada en corvinas en plena espermatogénesis y en espermiación (desde no detectable a 1047 pg mL -1 ). Los resultados obtenidos in vitro junto a los cambios a nivel plasmático sugieren que la 17,20β-P es un buen candidato a proponer como esteroide involucrado en la regulación del proceso de maduración testicular de la corvina. La función del cortisol a nivel testicular debería ser mejor estudiada en las etapas finales del desarrollo testicular de esta especie.

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Growth Hormone (GH) and Gonadotropin Subunit Gene Expression and Pituitary and Plasma Changes during Spermatogenesis and Oogenesis in Rainbow Trout (Oncorhynchus mykiss)

Cited by 266 publications

References 67 publications

Molecular characterization of Chinese sturgeon gonadotropins and cellular distribution in pituitaries of mature and immature individuals

Molecular characterization of Chinese sturgeon gonadotropins and cellular distribution in pituitaries of mature and immature individuals

Changes in Sex Steroids, Growth Hormone, and Insulin‐Like Growth Factor‐I during Ovarian Development in Rainbow Trout Cultured in a Water Recirculating System with Continuous Light

17,20β-P and cortisol are the main in vitro metabolites of 17-hydroxy-progesterone produced by spermiating testes of Micropogonias furnieri (Desmarest, 1823) (Perciformes: Sciaenidae)

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