Abstract:Salivary diagnosis is a developing area in clinical chemistry and dentistry. Cortisol analyses from saliva have been used in pediatric practice and as doping tests. Growth hormone (hGH), also a stress hormone, has not been analyzed from saliva. We studied the serum and saliva of 51 healthy subjects. The samples were taken at 8:00 in the morning after 12 h fasting. Cortisol concentrations were analyzed using RIA. An immunoradiometric assay was applied for analyzing serum and salivary hGH. The validity of this m… Show more
“…• There is autonomous production of ghrelin by the salivary glands, as has been shown for other hormones and growth factors (35,36 ). We could demonstrate production of ghrelin mRNA in all major salivary glands and in oral keratinocytes.…”
Background:We investigated whether ghrelin is present in human saliva, is produced by salivary glands, and physiologic consequences of these findings. Methods: Expression of ghrelin and specific receptor mRNA was determined by PCR. Proteins were identified by immunoblotting and size-exclusion fast protein liquid chromatography (FPLC) with consecutive RIA. Specific RIAs were used for quantification of salivary total and bioactive ghrelin. Distribution of ghrelin was investigated by immunohistochemistry in cryosections of the salivary glands. The effect of ghrelin on incorporation of 5-bromo-2-deoxyuridine as a measure of cell proliferation was investigated in primary oral keratinocytes. Results: Ghrelin is produced by the salivary glands. The hormone was identified in saliva and glands by immunoblotting and by FPLC fractionation of saliva. Immunohistochemistry demonstrated ghrelin distribution in the salivary glands. The receptor was also produced by the glands and by oral keratinocytes and was shown to be functional. Comparison of total ghrelin values for healthy individuals (body mass index, 18 -27 kg/m 2 ) showed significantly lower concentrations in saliva than in serum (P <0.01). The correlation between both matrices was r 2 ؍ 0.56 (P <0.001) with a negative correlation to body mass index (r 2 ؍ 0.314; P <0.01). Bioactive acylated ghrelin was also present in saliva. Incubation of keratinocytes with ghrelin led to signifi-
“…• There is autonomous production of ghrelin by the salivary glands, as has been shown for other hormones and growth factors (35,36 ). We could demonstrate production of ghrelin mRNA in all major salivary glands and in oral keratinocytes.…”
Background:We investigated whether ghrelin is present in human saliva, is produced by salivary glands, and physiologic consequences of these findings. Methods: Expression of ghrelin and specific receptor mRNA was determined by PCR. Proteins were identified by immunoblotting and size-exclusion fast protein liquid chromatography (FPLC) with consecutive RIA. Specific RIAs were used for quantification of salivary total and bioactive ghrelin. Distribution of ghrelin was investigated by immunohistochemistry in cryosections of the salivary glands. The effect of ghrelin on incorporation of 5-bromo-2-deoxyuridine as a measure of cell proliferation was investigated in primary oral keratinocytes. Results: Ghrelin is produced by the salivary glands. The hormone was identified in saliva and glands by immunoblotting and by FPLC fractionation of saliva. Immunohistochemistry demonstrated ghrelin distribution in the salivary glands. The receptor was also produced by the glands and by oral keratinocytes and was shown to be functional. Comparison of total ghrelin values for healthy individuals (body mass index, 18 -27 kg/m 2 ) showed significantly lower concentrations in saliva than in serum (P <0.01). The correlation between both matrices was r 2 ؍ 0.56 (P <0.001) with a negative correlation to body mass index (r 2 ؍ 0.314; P <0.01). Bioactive acylated ghrelin was also present in saliva. Incubation of keratinocytes with ghrelin led to signifi-
“…Sporadic reported investigations have demonstrated the presence of these 2 peptides in saliva (74,75 ) at very low concentrations (‰ of plasma concentrations).…”
Section: Salivary Hormones In Sports Medicinementioning
BACKGROUND: Saliva, which offers a noninvasive and stress-free alternative to plasma and serum, is a widely accepted sample source for analysis of steroids and also of certain amines and peptides. In recent years, numerous publications have described the use of salivary hormone analysis in many fields of clinical and basic research.CONTENT: This review provides an overview of the current applications of salivary hormone analysis. A description of the different modes of hormone entry into saliva is followed by a detailed description of analytical methods and approaches for reliable collection of saliva, including several interesting applications in diverse fields including psychiatry, stress research, clinical endocrinology, sports medicine, and veterinary medicine.
“…While one report of T4 in saliva found no correlation with serum levels [91], another study showed a significant correlation between salivary and serum T4 [92]. Salivary growth hormone was shown in one study to generally correlate with serum levels, but since its concentrations are 1000 fold lower than serum values, testing saliva for GH is difficult [93]. Salivary IGF-1 has been found to generally reflect growth hormone levels, being low in GH-deficient subjects and high in acromegalics when compared to controls [94]; however, its sensitivity for diagnosing acromegaly is very poor, with nearly half of acromegalic patients in one study having salivary IGF-1 concentrations within the normal range [95].…”
There is growing epidemiological interest in hormones as predictors of chronic diseases. The correct handling and analysis of hormones can be cumbersome, and great care must be taken in these processes in order to gain the most information possible. Given differences in sampling, processing, and stability of the various hormonal assays, we sought to provide a comprehensive review to aid future epidemiological research. We have coupled a thorough literature search with our own analytical experience to outline common laboratory problems one must consider in analyzing the hormones of the hypothalamic-pituitary axis. In addition, we describe the benefits and limitations of using alternative media--including urine, saliva, and blood spots on filter paper--to measure endocrine hormones in epidemiological studies.
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