Growth factors in cultured pterygium fibroblasts: immunohistochemical and ELISA analysis

Kria, L.; Ohira, Akihiro; Amemiya, Tsugio

doi:10.1007/s004170050144

Cited by 99 publications

(73 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…2,3 Several environmental factors that may induce ocular inflammation and various cytokines have been reported to be involved in the development of pterygium. [4][5][6] However, the precise mechanism of pterygium pathogenesis is unclear.…”

Section: Introductionmentioning

confidence: 99%

Endothelial progenitor cells in pterygium pathogenesis

Lee

Song

et al. 2006

Eye

View full text Add to dashboard Cite

Purpose The pathogenesis of pterygium is not well known, and controversy exists about the cell origins and the nature of initial trigger required for its development. We investigated whether endothelial progenitor cells (EPCs) are involved in pathogenesis of pterygium and the mechanism underlying the selective recruitment of EPCs during this process. Methods We studied 13 normal controls and 28 pterygium patients (primary (n ¼ 15), recurrent (n ¼ 13)). Substance-P, vascular endothelial growth factor (VEGF), and stem cell factor (SCF) were measured in plasma and tears using ELISA, and circulating CD34 þ and c-kit þ mononuclear cells (MNCs) by flow cytometry. Anterior segment fluorescein angiography (FAG) was performed to evaluate hypoxic conditions in the early stage of pterygium. Surgically removed pterygial tissues were analyzed immunohistochemically using the progenitor cell markers, CD34, c-kit, VEGFR-1, and VEGFR-2. Results Anterior segment FAG findings showed an increase in non-perfusion areas and attenuated vessels in the nasal limbus during early-stage pterygium. Circulating CD34 þ MNCs and c-kit þ MNCs were increased in pterygium groups compared with normal controls. Systemic and local cytokines including SP, VEGF, and SCF in pterygium groups were also elevated and showed positive correlations with CD34 þ and c-kit þ MNC numbers. Immunohistochemical analysis of pterygium showed strong progenitor cell marker immunoreactivities. Conclusions EPCs might be involved in pterygium development, and ocular hypoxia triggers this neovascularization by recruiting EPCs derived from the bone marrow via the production of systemic and local cytokines.

show abstract

Section: Introductionmentioning

confidence: 99%

Endothelial progenitor cells in pterygium pathogenesis

Lee

Song

et al. 2006

Eye

View full text Add to dashboard Cite

show abstract

“…UV-mediated genetic trauma may also affect the expression of various cytokines, growth factors and growth factor receptors (6). The presence of such factors in pterygium has been evaluated in previous studies using various methods including immunohistochemistry and ELISA in cell cultures (12). This study focused on the evaluation of five growth factors (TGFB1, VEGFA, FGF, IGF1 and EGF), which had previously been shown to be individually affected in pterygium (6), and employed qRT-PCR to detect possible alterations in the expression of these factors in pterygium, compared with normal conjunctiva as well as potential clinical associations of molecular findings.…”

Section: Discussionmentioning

confidence: 99%

Growth factor expression in ophthalmic pterygia and normal conjunctiva

Detorakis

Zaravinos²,

Spandidos³

2010

Int J Mol Med

View full text Add to dashboard Cite

Abstract. The expression of growth factors in ophthalmic pterygium and phenotypically normal conjunctiva was examined and correlated with the clinical findings. Fifteen specimens of ophthalmic pterygia and 8 specimens of phenotypically normal conjunctiva were examined. Total RNA was extracted from all specimens and mRNA levels for transforming growth factor (TGFB1), vascular endothelial growth factor (VEGFA), basic fibroblast growth factor (FGF2), epidermal growth factor (EGF) and insulin-like growth factor (IGF1) were measured using real-time reversetranscription PCR (qRT-PCR). Differences in the expression of these factors between pterygium and conjunctival specimens were examined, as well as correlations between RNA levels and clinical parameters. mRNA levels for VEGFA and FGF2 were significantly higher in pterygium, compared with conjunctival specimens, whereas the respective differences for other factors examined were statistically not significant. The mRNA levels for VEGFA and FGF2 were also significantly higher in recurrent compared with primary pterygium. Correlations between the mRNA levels and other clinical parameters were statistically not significant for the growth factors examined. The higher levels of FGF2 or VEGFA mRNA in pterygium imply that these factors may be involved in the pathogenesis or clinical behavior of the pterygium, including postoperative recurrence. VEGFA expression may have potential for use in the selective treatment of pterygium with anti-VEGFA agents.

show abstract

“…A few studies that differentiated primary and recurrent pterygia reported that the levels of VEGF, basic fibroblast growth factor, and substance P were significantly higher in recurrent disease (9) . Markers for vascular endothelial cells such as CD31+ are increased in pterygium, which supports angiogenesis (10) .…”

Section: Introductionmentioning

confidence: 99%

The role of mast cells in vascularized recurrent pterygium

Çelebi¹,

Özbey²,

Mirza³

2014

Arquivos Brasileiros De Oftalmologia

View full text Add to dashboard Cite

Objective: To determine and compare the mast cell count in primary and recurrent vascularized pterygium, and in normal bulbar conjunctiva. Methods: The study included 22 patients with primary pterygium (PP group) and 28 patients with vascularized recurrent pterygium (VRP group) that underwent excision via the limbal conjunctival autograft technique. Normal conjunctiva samples were collected from the superotemporal bulbar conjunctival region, just temporal to the site from which the autograft conjunctival tissue was harvested. The total number of mast cells in the pterygium (primary and recurrent) and control tissue samples was calculated microscopically using 1% toluidine blue stain under 400× magnification. Results:The mean mast cell count in primary and vascularized recurrent pterygium tissue was 7.45 ± 2.06 mm -2 and 16.11 ± 4.33 mm -2 , respectively, and the difference was significant (independent samples t-test, P<0.001). The mean mast cell count in pterygium tissue was significantly higher than that in normal conjunctiva tissue in both groups (Student's t-test, P<0.001). Conclusion: An increase in the number of mast cells might play a role in the pathogenesis of recurrent pterygium. Determination of a mast cell count cut-off value could be of diagnostic significance for recurrent pterygium.

show abstract

Growth factors in cultured pterygium fibroblasts: immunohistochemical and ELISA analysis

Abstract: The strong immunoreactivity and the release of b-FGF in cultured fibroblasts of recurrent pterygia suggest that fibroblasts may play an important role in the recurrence of pterygium.

Cited by 99 publications

References 20 publications

Endothelial progenitor cells in pterygium pathogenesis

Endothelial progenitor cells in pterygium pathogenesis

Growth factor expression in ophthalmic pterygia and normal conjunctiva

The role of mast cells in vascularized recurrent pterygium

Contact Info

Product

Resources

About