Growth factors for Corynebacterium diphtheriae. 4. Preparation from yeast

Growth Factors for Corynebacterium diphtheriae Strain Dundee

“…Also, the growth-initiating activity (as distinct from any further growth-stiniulatory effect) of a fish-liver concentrate has been correlated with its purine content. These findings differ considerably from the activity of ninhydrin-positive material found by Chattaway et al (1949 by a factor of lo4, suggests some fundamental difference in function. Whatever the correct explanation may be, it is apparent that the fish-liver concentrate contains some additional material which, whilst having no ability to promote growth in the absence of purines, supplements growth in their presence.…”

“…Basal medium. This was essentially the same as that of Chattaway et al (1949), with the addition of pteroylglutamic acid, thiamine and cobaltous chloride ; hydroxyproline was absent. Since the exact amino acid requirements of the Dundee organism were not known, equal concentrations of each amino acid were used with the exception of glutamic acid (Mueller, 1940) and tyrosine (solubility considerations).…”

“…The addition of purines to a che'mically defined medium almost identical with that of Chattaway et al (1949) has thus been shown to allow the growth of Corynebacterium diphtheriae strain Dundee. Also, the growth-initiating activity (as distinct from any further growth-stiniulatory effect) of a fish-liver concentrate has been correlated with its purine content.…”

“…Chattaway, Happold & Sandford (1944) and Chattaway, Dolby, Hall & Happold (1949) reported the presence in liver and yeast concentrates of a factor or factors essential for the growth of certain intermediate and gravis strains of Cwynebucterium dipintherim on an otherwise chemically defined medium. Working with acid hydrolysates of brewers' yeast they obtained chromatographic data which suggested that the active material was associated with four ninhydrin-reacting substances.…”

“…In view of the previously reported activity of ninhydrin-positive material (Chattaway et al 1949), it was necessary to determine whether purines were the active agents in the fish-liver concentrate and, if so, whether they accounted for all of the activity. A modification of the copper-precipitation technique of Vendrely (1947) was applied to the -concentrate before and after acid hydrolysis, so as to distinguish between free and bound purines.…”