Growth factors and their action in vivo and in vitro

Abstract

The molecular nature and mechanism of action of several of the growth factors including epidermal growth factor, fibroblast growth factor and platelet-derived growth factor is reviewed. The ectopic production of growth factors may be important in the growth development of certain tumours. In addition, attention is directed to the importance of the extracellular matrix in controlling cell proliferation and differentiation.

A new simplified technique for producing platelet-rich plasma: a short technical note

“…The effects of these growth factors on cell behavior and bone-healing sequences have been thoroughly studied [4,5,7,11,15,20,21].…”

“…Under the influence of other cytokines and local environmental conditions, such as pH, oxygen tension, and micromotion, these cells undergo a differentiation into osteoblasts or chondroblasts. Collagen and protein synthesis by osteoblasts is also stimulated by PDGF, but also requires the presence of IGF-I [7]. PDGF probably enhances the secretion of IGF-I by the osteoblasts and mesenchymal stem cells, which then accelerates the formation of a collagenous matrix [11].…”

Do autologous growth factors enhance transforaminal lumbar interbody fusion?

“…Recent studies have reported the efficacy of bone morphogenetic protein (BMP) in facilitating spinal fusion [5]. The beneficial effects of platelet-derived growth factor (PDGF) on bone formation in vitro has also been described previously [9,10,18,20,22,34,36,37], while in vivo stimulation of bone formation by transforming growth factor-β (TGF-β) has been shown in animal models [20,30,33]. Both PDGF and TGF-β have mitogenic effects on fibroblasts, osteoblasts, and mesenchymal cells by the stimulation of DNA synthesis and cell replication [12].…”

Overexpression of TGF-β by infiltrated granulocytes correlates with the expression of collagen mRNA in pancreatic cancer

“…The desmoplastic reaction has been reported to be stimulated by various growth factors including epidermal growth factor (EGF) (Gospodarowicz, 1983), platelet-derived growth factor (PDGF) (Gospodarowicz, 1983;Tahara, 1990), connective tissue growth factor (CTGF) (Frazier et al, 1996), fibroblast growth factor (FGF) (Gospodarowicz, 1983;Powers et al, 2000) and transforming growth factor-b (TGF-b) (Roberts et al, 1986;Fine and Goldstein, 1987;Sappino et al, 1990;McCartney-Francis and Wahl, 1994). In various malignancies including pancreatic cancer, overexpression of these growth factors is frequently observed (Korc et al, 1992;Friess et al, 1993;Yamanaka et al, 1993;Ebert et al, 1995;Gold, 1999;Wenger et al, 1999) and has been associated with a significant decrease in the survival and advanced tumour stage .…”