Growth factors and corneal epithelial wound healing

Yu, Fei; Yin, Jia; Xu, Ke‐Ping; Huang, Jenny

doi:10.1016/j.brainresbull.2009.08.024

Cited by 170 publications

(141 citation statements)

References 138 publications

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“…29,30 PDGF-AB, EGF, vascular endothelial growth factor, insulin-like growth factor 1 and transforming growth factor-b1 are involved in the network of proliferation, differentiation, migration and apoptosis of corneal cells contributing to epithelial renewal. [31][32][33] There is in vitro evidence that PRG is richer in PDGF-AB, EGF and transforming growth factor-b1 than serum and better stimulates growth of corneal epithelial cells. 22 This encouraged the use of PRGs for treating DES, corneal lesions, neurotrophic keratitis and persistent corneal epithelial defects with promising results.…”

Section: Discussionmentioning

confidence: 99%

Autologous platelet lysate for treatment of refractory ocular GVHD

Pezzotta

Fante

Scudeller

et al. 2012

Bone Marrow Transplant

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Current treatment of ocular GVHD (oGVHD), represented by systemic immunosuppressive regimens and local therapies (mainly artificial tears and corticosteroids), gives unsatisfactory results. We investigated the safety and efficacy of autologous plasma rich in PDGFs to treat oGVHD unresponsive to standard medications. A total of 23 patients with refractory oGVHD (grade II-IV) unresponsive to standard therapy were treated with autologous plasma rich in PDGFs eye drops (PRGD) four times/day for 6 months. Symptoms and signs (best visual acuity, Schirmer's test and tear break up time (TBUT), evaluation of the anterior segment and fluorescein and lissamine staining) were always assessed by the same ophthalmologist. Patients were defined as 'responders' when showing improvement for total complaints and at least one sign. At 30 days of treatment, 17 patients (73.9%) were classified as responders. The symptom that improved most was photophobia (improved in 19 patients, 82.6%). TBUT improved in 20 patients (86.9%) and anterior segment score in 19 patients (82.6%). Response was maintained over time. No serious adverse events occurred. PRGD proved to be safe and effective in treating oGVHD and may be a valid treatment option from the early stages of the disease to avoid irreversible ocular damage.

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Section: Discussionmentioning

confidence: 99%

Autologous platelet lysate for treatment of refractory ocular GVHD

Pezzotta

Fante

Scudeller

et al. 2012

Bone Marrow Transplant

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“…5,6 EGF disekresi oleh trombosit, makrofag dan fibroblas, serta berperan dalam paracrine fashion pada sel epitel. 5,7 Epidermal growth factor juga memegang peranan penting dalam pembentukan granulasi jaringan dan secara in vitro dapat merangsang migrasi dan proliferasi keratinosit. 7,8 Terapi luka dengan EGF topikal akan meningkatkan proses penyembuhan luka dan mempersingkat waktu penyembuhan.…”

Section: Pendahuluanunclassified

“…5,7 Epidermal growth factor juga memegang peranan penting dalam pembentukan granulasi jaringan dan secara in vitro dapat merangsang migrasi dan proliferasi keratinosit. 7,8 Terapi luka dengan EGF topikal akan meningkatkan proses penyembuhan luka dan mempersingkat waktu penyembuhan. 5,7 Dalam upaya meningkatkan aktivitas serum otologus maka perlu ditambahkan hEGF dalam berbagai variasi konsentrasi sehingga diperoleh konsentrasi yang optimal dalam mempercepat proses penyembuhan luka.…”

Section: Pendahuluanunclassified

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Serum Otologus dan human Epidermal Growth Factor (hEGF) Mempercepat Proliferasi dan Migrasi Keratinosit pada Proses Re-Epitelisasi

Agung¹,

Maksum²,

Toto³

2016

mkb

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AbstrakPenggunaan serum otologus merupakan pendekatan terapeutik yang direkomendasikan untuk mengobati beberapa jenis penyakit yang bersifat kronis, menahun bahkan dapat bertahan seumur hidup. Serum otologus mengandung epidermal growth factor (EGF) yang dapat menstimulasi proses migrasi dan proliferasi keratinosit pada proses re-epitelisasi dalam penyembuhan luka. Penambahan hEGF pada serum otologus dilakukan sebagai upaya untuk mempercepat proses penyembuhan luka. Pengujian aktivitas dilakukan dengan mengukur proliferasi dan migrasi sel keratinosit menggunakan cell line HaCaT. Proliferasi sel diukur dengan metode Water Soluble Tetrazolium-8 (WST-8) dan migrasi sel diukur dengan metode Scratch Assay. Variasi konsentrasi hEGF yang ditambahkan adalah 0,5; 1; 5; 10; 25 dan 50 ng/mL. Terdapat perbedaan yang bermakna pada hasil proliferasi sel HaCaT yang ditambahkan hEGF dan konsentrasi optimal pada penambahan hEGF 25 ng/mL (p<0,05), sedangkan penambahan variasi konsentrasi hEGF pada serum otologus tidak menunjukkan perbedaan yang bermakna terhadap proliferasi sel HaCaT (p>0,05 Autologous Serum and human Epidermal Growth Factor (hEGF) Accelerate Keratinocyte Proliferation and Migration during Re-epithelialization Process AbstractAutologous serum is used as a therapeutic approach recommended to treat certain types of chronic diseases that can even last a lifetime. Autologous serum contains Epidermal Growth Factor (EGF), which can stimulate the migration and proliferation of keratinocytes in the re-epithelialization process in wound healing. The addition of hEGF on autologous serum is a part of efforts to accelerate the wound healing process. Tests were performed by measuring proliferation and migration activities of keratinocytes cells using HaCaT cell line. The cell proliferation was measured using Water Soluble Tetrazolium-8 (WST-8) method while the cell migration was measured using Scratch Assay method. Variations in the concentration of hEGF added were 0.5, 1, 5, 10, 25, and 50 ng / mL. There were significant differences in the results of cell proliferation in hEGF-added HaCaTcells and the optimum concentration was seen in 25 ng/mL hEGF group (p <0.05). On the contrary, the addition of various concentrations of hEGF in autologous serum resulted in no significant difference in HaCaT cell proliferation (p>0 , 05). The addition of hEGF and autologous serum showed a visible role in accelerating the pace of cell migration. Within 18 hours, the percentage of cell migration in HaCaT cells added by hEGF and autologous serum reaches 55-70% and then 65-80% within 24 hours while HaCaT cells that receive hEGF only only reaches 25-45% cell migration which increases to 35-70% within 24 hours. [MKB. 2016;48(4):205-10]

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“…Keratinocyte growth factor (KGF), a monomeric peptide that belongs to the fibroblast growth factor (FGF) family (Branski et al, 2007), is produced by cells of mesenchymal origin and mediates epithelial cell proliferation and differentiation in a variety of tissues such as lung and skin (Auf dem Keller et al, 2004;Yu et al, 2010). This paracrine action of KGF on epithelial cells is mediated through the KGF receptor, a splice variant of the FGF-2 receptor encoded by the FGF receptor gene.…”

Section: Introductionmentioning

confidence: 99%

The Effects of Adenoviral Transfection of the Keratinocyte Growth Factor Gene on Epidermal Stem Cells: an In Vitro Study

Liang

Zhao

et al. 2013

Molecules and Cells

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Epidermal stem cells (ESCs) are characterized as slowcycling, multi-potent, and self-renewing cells that not only maintain somatic homeostasis but also participate in tissue regeneration and repair. To examine the feasibility of adenoviral vector-mediated keratinocyte growth factor (KGF) gene transfer into in vitro-expanded ESCs, ESCs were isolated from samples of human skin, cultured in vitro, and then transfected with recombinant adenovirus (Ad) carrying the human KGF gene (AdKGF) or green fluorescent protein gene (AdGFP). The effects of KGF gene transfer on cell proliferation, cell cycle arrest, cell surface antigen phenotype, and β-catenin expression were investigated. Compared to ESCs transfected with AdGFP, AdKGFtransfected ESCs grew well, maintained a high proliferative capacity in keratinocyte serum-free medium, and expressed high levels of β-catenin. AdKGF infection increased the number of ESCs in the G0/G1 phase and promoted ESCs entry into the G2/M phase, but had no effect on cell surface antigen phenotype (CD49f + /CD71 -). The results suggest that KGF gene transfer can stimulate ESCs to grow and undergo cell division, which can be applied to enhance cutaneous wound healing.

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Growth factors and corneal epithelial wound healing

Cited by 170 publications

References 138 publications

Autologous platelet lysate for treatment of refractory ocular GVHD

Autologous platelet lysate for treatment of refractory ocular GVHD

Serum Otologus dan human Epidermal Growth Factor (hEGF) Mempercepat Proliferasi dan Migrasi Keratinosit pada Proses Re-Epitelisasi

The Effects of Adenoviral Transfection of the Keratinocyte Growth Factor Gene on Epidermal Stem Cells: an In Vitro Study

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