Growth factor regulation of bovine satellite cell growth in vitro.

Abstract

This study examined the effects of basic fibroblast growth factor (bFGF), insulin-like growth factor I (IGF-I) and transforming growth factor beta (TGF-beta) on the proliferation and differentiation of primary bovine satellite cells (BSC) in vitro. Individually, these three factors had the following effects on satellite cells: bFGF stimulated proliferation (P less than .01) but inhibited differentiation (P less than .05); IGF-I had no effect on proliferation but stimulated differentiation (P less than .01); and TGF-beta inhibited both proliferation and differentiation (P less than .01). When combined, the following effects were observed: maximum stimulation of proliferation (P less than .01) occurred in the presence of bFGF and IGF-I and differentiation was not stimulated; TGF-beta and bFGF continued to inhibit differentiation (P less than .01), but in the presence of bFGF, TGF-beta stimulated proliferation (P less than .01). No stimulation was observed in the presence of TGF-beta and IGF-I. Bovine satellite cells respond to these three growth factors that have been shown to regulate the activity of other myogenic cells, and in most instances, the responses among cells from various species are similar. These experiments indicate that the interactions of growth factors may be critical in regulating bovine satellite cell activity.

Overexpression of the type-1 insulin-like growth factor receptor increases ligand-dependent proliferation and differentiation in bovine skeletal myogenic cultures

“…5B). Treatment of both Bov-LISN and Bov-LNL6 cultures with TGF-P resulted in 50% decreases in MyHC expression, in agreement with previous observations by Greene and Allen (1991) that TGF-P inhibits bovine myoblast differentiation. As in the dose-response curves generated without TGF-p, the IGF-I dose-response curve of Bov-LISN MyHC expression was more pronounced for both ascending and descending limbs compared to Bov-LNL6 cultures.…”

Transforming growth factor-β and myostatin signaling in skeletal muscle

“…TGF-␤ represses skeletal muscle specific gene expression and has also been reported to modulate proliferation in satellite cells (3,12,19,24,47,59). Taken together, these studies indicate that TGF-␤ reprograms gene expression in muscle cells resulting in an alteration of proliferative control and a potent inhibition of the program of gene expression underlying myogenic differentiation.…”

Primary human muscle precursor cells obtained from young and old donors produce similar proliferative, differentiation and senescent profiles in culture

“…The ability of TGF-b1 to inhibit myoblast differentiation and to depress proliferation has been documented (Massague et al, 1986;Allen & Boxhorn, 1987;Greene & Allen, 1991). More recently, human MPCs were cultured in the presence of 25 ng ml À1 human recombinant TGF-b1 (Carlson et al, 2009b).…”