Growth Factor Effects on the Expression of Collagenase and TIMP‐1 in Periodontal Ligament Cells

Abstract

The fibroblast is a prominent cellular component of the periodontal ligament. It is believed to play an important role in collagen metabolism in health and disease. The turnover of collagen in the periodontal ligament is believed to be controlled by the balance between collagen synthesis and degradation. The family of matrix metalloproteinases and their inhibitors is one of the mechanisms which regulates this balance. The factors that regulate the synthesis of collagenase and its inhibitor, TIMP-1, by the periodontal ligament cell are poorly understood. The present study was undertaken to assess the effect of interleukin-1 beta (IL-1 beta), platelet-derived growth factor (PDGF), and transforming growth factor-beta 1 (TGF-beta) on the expression of collagenase (MMP-1) and TIMP-1 mRNA in periodontal derived fibroblasts using reverse transcription polymerase chain reaction (RT-PCR). Early passage periodontal ligament derived fibroblasts were treated with IL-1 beta (10 and 100 pg/ml), two isoforms of PDGF, -AA and -BB (4 and 20 ng/ml) and TGF-beta (1 and 10 ng/ml). Treatment with growth factors from 2 to 24 hours revealed that the largest effects on MMP-1 mRNA occurred after 24 hours. IL-1 beta induced a 5 to 9 fold increase in MMP-1 mRNA. The two isoforms of PDGF had less of an effect (3 to 5 fold) on MMP-1 mRNA whereas TGF-beta induced a 25 to 50% decrease in the expression of this message. None of the growth factors had an effect on TIMP-1 mRNA expression.(ABSTRACT TRUNCATED AT 250 WORDS)

Repetitive mechanical stretching modulates IL-1β induced COX-2, MMP-1 expression, and PGE2 production in human patellar tendon fibroblasts

“…IL-1β is a potent pro-inflammatory cytokine produced mainly by macrophages in injured tissues and upregulates the expression of other inflammatory mediators, including COX-2, IL-6, MMP-1, and MMP-3. In fibroblasts, for example, IL-1β induces the expression of MMPs (Tewari et al, 1994;Alvares et al, 1995), which could initiate the degradation and remodeling of tissues (e.g., tendons) in response to mechanical loading (Vailas et al, 1985). COX-2 is a major marker of tissue inflammation (Gilroy et al, 1998;Lipsky, 1999) that converts arachidonic acid into prostaglandins, such as PGE 2 .…”

Resveratrol Modulates Interleukin-1β-induced Phosphatidylinositol 3-Kinase and Nuclear Factor κB Signaling Pathways in Human Tenocytes

“…These tendon conditions are accompanied by inflammation through up-regulation of proinflammatory cytokines, COX-2, matrix metalloproteinase (MMP) 2 expression, and prostaglandin E 2 (PGE 2 ) production. This could initiate the degradation and remodeling of tendons in response to mechanical loading (1,2).…”

MMP and TIMP production in periodontal ligament fibroblasts stimulated byPrevotella nigrescenslipopolysaccharide

“…The turnover of collagen in the periodontal ligament is controlled by the balance between collagen synthesis and degradation. The family of matrix metalloproteinases and their inhibitors is one of the mechanisms which regulate this balance 36) . In this study, we quantified the MMP-1 mRNAs extracted from PDL fibroblasts after LPS stimulation, and normalized with GAPDH mRNA levels 37) .…”

“…Alvares et al reported that IL-1βexerted profound effect in MMP-1 synthesis in PDL fibroblasts, but no effects in TIMP-1 at the mRNA level. They suggested that cytokines differentially and specifically regulate expression of collagenase mRNA in human PDL 36) . LPS is known to exert profound effect on the protein expression by host cells.…”