Growth Factor Delivery Approaches in Hydrogels

Silva, Amanda; Richard, Cyrille; Bessodes, Michel; Scherman, Daniel; Merten, Otto‐Wilhelm

doi:10.1021/bm801103c

Cited by 248 publications

(179 citation statements)

References 96 publications

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“…However, the desired cellular response is currently triggered by several approaches such as controlled release of signaling molecules, use of genetically engineered cells that constitutively express the desired factors, or coupling signaling molecules to the scaffolds. 1,2 These approaches have significant drawbacks such as inconsistent release kinetics, unpredictable diffusion rates of released molecules, risk of oncogenic transformation of transfected cells, and loss of activity of coupled molecules. 1,2 Often times, scaffolds are selected based on their ability to promote adhesion and proliferation of the desired cell types.…”

Section: Introductionmentioning

confidence: 99%

“…1,2 These approaches have significant drawbacks such as inconsistent release kinetics, unpredictable diffusion rates of released molecules, risk of oncogenic transformation of transfected cells, and loss of activity of coupled molecules. 1,2 Often times, scaffolds are selected based on their ability to promote adhesion and proliferation of the desired cell types. Although several of the scaffolds used for tissue engineering applications are derived from extracellular matrix (ECM) proteins or polymers designed to mimic ECM proteins, they do not mimic the native ECM, and therefore, in vivo-like behavior of the embedded cells cannot be obtained.…”

Section: Introductionmentioning

confidence: 99%

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Biomimetic Extracellular Matrix-Incorporated Scaffold Induces Osteogenic Gene Expression in Human Marrow Stromal Cells

Ravindran

Gao

Kotecha

et al. 2012

Tissue Engineering Part A

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Engineering biomaterials mimicking the biofunctionality of the extracellular matrix (ECM) is important in instructing and eliciting cell response. The native ECM is highly dynamic and has been shown to support cellular attachment, migration, and differentiation. The advantage of synthesizing an ECM-based biomaterial is that it mimics the native cellular environment. However, the ECM has tissue-specific composition and patterned arrangement. In this study, we have employed biomimetic strategies to develop a novel collagen/chitosan template that is embedded with the native ECM of differentiating human marrow stromal cells (HMSCs) to facilitate osteoblast differentiation. The scaffold was characterized for substrate stiffness by magnetic resonance imaging and nanoindentation and by immunohistochemical analysis for the presence of key ECM proteins. Gene expression analysis showed that the ECM scaffold supported osteogenic differentiation of undifferentiated HMSCs as significant changes were observed in the expression levels of growth factors, transcription factors, proteases, receptors, and ECM proteins. Finally, we demonstrate that the scaffold had the ability to nucleate calcium phosphate polymorphs to form a mineralized matrix. The results from this study suggest that the threedimensional native ECM scaffold directly controls cell behavior and supports the osteogenic differentiation of mesenchymal stem cells.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Biomimetic Extracellular Matrix-Incorporated Scaffold Induces Osteogenic Gene Expression in Human Marrow Stromal Cells

Ravindran

Gao

Kotecha

et al. 2012

Tissue Engineering Part A

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“…Hydrogel, which is defined as a three-dimensional polymer network capable of absorbing an aqueous solvent [15], was used as a biopolymer vehicle in previous studies [16][17][18]. The composition of hydrogels can be natural polymers or synthetic polymers.…”

Section: Introductionmentioning

confidence: 99%

Synthesis of Gelatin-γ-Polyglutamic Acid-Based Hydrogel for the In Vitro Controlled Release of Epigallocatechin Gallate (EGCG) from Camellia sinensis

et al. 2013

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Abstract:The antioxidant property and other health benefits of the most abundant catechin, epigallocatechin gallate (EGCG), are limited because of poor stability and permeability across intestine. Protecting the EGCG from the harsh gastrointestinal tract (GIT) environment can help to increase its bioavailability following oral administration. In this study, EGCG was loaded to hydrogel prepared from ionic interaction between an optimized concentration of gelatin and γ-polyglutamic acid (γ-PGA), with ethylcarbodiimide (EDC) as the crosslinker. Physicochemical characterization of hydrogel was done using Fourier transform-infrared spectroscopy (FT-IR), differential scanning calorimetry (DSC) and scanning electron microscopy (SEM). The dependence of the swelling degree (SD) of the hydrogel to the amount of gelatin, γ-PGA, EDC, swelling time and pH was determined. A high SD of the crosslinked hydrogel was noted at pH 4.5, 6.8 OPEN ACCESSPolymers 2014, 6 40 and 9.0 compared to pH 7.4, which describes pH-responsiveness. Approximately 67% of the EGCG from the prepared solution was loaded to the hydrogel after 12 h post-loading, in which loading efficiency was related to the amount of EDC. The in vitro release profile of EGCG at pH 1.2, 6.8 and 7.4, simulating GIT conditions, resulted in different sustained release curves. Wherein, the released EGCG was not degraded instantly compared to free-EGCG at controlled temperature of 37 °C at different pH monitored against time. Therefore, this study proves the potential of pH-responsive gelatin-γ-PGA-based hydrogel as a biopolymer vehicle to deliver EGCG.

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“…Injectable hydrogels are three-dimensional polymer networks extensively swollen in water ( Figure 4) [44], and represent a powerful delivery system for cardiac repair, since they are tri-dimensional networks that mimic the extracellular matrix and reproduce the natural environment and, in addition, they can be administered using non-invasive techniques like cardiac catheterization, thanks to their liquid-gel controllable nature [45]. In accordance with this potential, hydrogels have been developed using a long list of biomaterials.…”

Section: Hydrogelsmentioning

confidence: 99%

Heart regeneration after myocardial infarction using synthetic biomaterials

Pascual-Gil

Garbayo

Díaz-Herráez

et al. 2015

Journal of Controlled Release

116

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Abstract:Myocardial infarction causes almost 7.3 million deaths each year worldwide. However, current treatments are more palliative than curative. Presently, cell and protein therapies are considered the most promising alternative treatments. Clinical trials performed until now have demonstrated that these therapies are limited by protein short half-life and by low transplanted cell survival rate, prompting the development of novel cell and protein delivery systems able to overcome such limitations. In this review we discuss the advances made in the last 10 years in the emerging field of cardiac repair using biomaterial-based delivery systems with focus on the progress made on preclinical in vivo studies. Then, we focus in cardiac tissue engineering approaches, and how the incorporation of both cells and proteins together into biomaterials has opened new horizons in the myocardial infarction treatment. Finally, the ongoing challenges and the perspectives for future work in cardiac tissue engineering will also be discussed.

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Growth Factor Delivery Approaches in Hydrogels

Cited by 248 publications

References 96 publications

Biomimetic Extracellular Matrix-Incorporated Scaffold Induces Osteogenic Gene Expression in Human Marrow Stromal Cells

Biomimetic Extracellular Matrix-Incorporated Scaffold Induces Osteogenic Gene Expression in Human Marrow Stromal Cells

Synthesis of Gelatin-γ-Polyglutamic Acid-Based Hydrogel for the In Vitro Controlled Release of Epigallocatechin Gallate (EGCG) from Camellia sinensis

Heart regeneration after myocardial infarction using synthetic biomaterials

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