Growth Differentiation Factor-9 and Stem Cell Factor Promote Primordial Follicle Formation in the Hamster: Modulation by Follicle-Stimulating Hormone1

Wang, Jinrong; Roy, Shyamal K.

doi:10.1095/biolreprod.103.023234

Cited by 106 publications

(86 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Studies on growth factors have demonstrated that FSH and EGF are not essential for primordial follicle activation [24][25][26] but are important for latter follicle development [1,14]. On the other hand, GDF-9 and bFGF can promote the initiation of primordial follicle development and stimulate early preantral follicle growth [5,6,8,[27][28][29]. Hayashi et al reported that GDF-9 can enhance the effect of FSH on follicular growth [4,5].…”

Section: Discussionmentioning

confidence: 99%

In vitro culture of sheep lamb ovarian cortical tissue in a sequential culture medium

Peng

Yang

Wang

et al. 2010

J Assist Reprod Genet

View full text Add to dashboard Cite

Purpose This study evaluates the effect of a sequential culture system on the follicular development of sheep lamb ovaries, aiming to establish an available in vitro culture system for ovarian culture. Methods Lamb ovarian cortical fragments were cultured on a steel mesh with a nitrocellulose membrane pre-coated by type1 collagen. Several culture media were used for the determinations, specifically, a control medium (α-MEM), a constant medium (control medium supplemented with 75 ng/mL human recombinant EGF, 200 mIU/mL sheep FSH, 100 ng/mL human recombinant GDF-9, and 100 ng/mL human recombinant bFGF), and a sequential medium (control medium supplemented with sequential growth factors added on different days). Ovarian tissues, both fresh and cultured, were processed for histological and apoptotic assays, while spent culture media were processed for hormone assays.Results It was found that the growth of lamb primordial follicles can be initiated during culture in vitro. Compared to the control medium, sequential culture medium significantly increased the percentage of secondary follicles in cultures, while the follicle and oocyte diameters of primary and secondary follicles were also observed to increase in this medium. The constant medium was found to increase the number and diameter of secondary follicles only 18 days after culture. After this same period of time, some normal antral follicles were found in the sequential medium, while a few abnormal antral-like follicles were found in the control medium. Moreover, sequential medium appeared to significantly increase estradiol and inhibin production, especially 10-18 days after culture. The highest percentage of normal follicles and the lowest apoptotic cell rates were observed in the sequential medium, suggesting that a sequential addition style of culture can improve follicle and tissue viability. Conclusions The sequential addition of FSH, EGF, GDF-9, and bFGF can stimulate primordial follicle transmittal into the later development stages, even as far as the antral stage, improve the survival rate of follicles, and maintain follicular viability.

show abstract

Section: Discussionmentioning

confidence: 99%

In vitro culture of sheep lamb ovarian cortical tissue in a sequential culture medium

Peng

Yang

Wang

et al. 2010

J Assist Reprod Genet

View full text Add to dashboard Cite

show abstract

“…Both BMP15 and GDF9 are secreted by the oocyte early in ovarian differentiation, suggesting that signals from the oocyte to the granulosa cells are important for cyst breakdown and follicle formation (Elvin et al 2000). Supporting the involvement of GDF9 in follicle formation, addition of GDF9 to hamster ovaries in organ culture promotes the formation of follicles (Wang & Roy 2004). Mouse ovaries exposed to another TGFB family member, activin A, have an increased number of primordial follicles (Bristol-Gould et al 2006).…”

Section: Growth Factors and Signaling Moleculesmentioning

confidence: 98%

“…Ovary organ culture studies have revealed a potential role for stem cell factor (SCF) in primordial follicle formation. When SCF is added to neonatal hamster ovaries in organ culture, primordial follicle formation is accelerated while an antibody against SCF inhibits follicle formation (Wang & Roy 2004). SCF and its receptor, the c-KIT receptor tyrosine kinase, have been well studied during several phases of ovarian development.…”

Section: Growth Factors and Signaling Moleculesmentioning

confidence: 99%

“…FSH is present in the serum of neonatal mice and hamsters (Vomachka & Greenwald 1979, Halpin et al 1986) and the FSH receptor is detected using RT-PCR in neonatal mouse ovaries (O'Shaughnessy et al 1994). Inactivation of FSH using an anti-FSH antibody results in the inhibition of primordial follicle formation in the hamster (Roy & Albee 2000), while addition of FSH to cultured ovaries results in acceleration of primordial follicle formation (Wang & Roy 2004). A recent study using mouse ovary organ culture also implicates FSH in promoting cyst breakdown and primordial follicle formation (Lei et al 2010).…”

Section: Hormonesmentioning

confidence: 99%

See 1 more Smart Citation

Follicular assembly: mechanisms of action

Pepling¹

2012

Reproduction

202

190

View full text Add to dashboard Cite

The differentiation of primordial germ cells (PGCs) into functional oocytes is important for the continuation of species. In mammals, PGCs begin to differentiate into oocytes during embryonic development. Oocytes develop in clusters called germ line cysts. During fetal or neonatal development, germ cell cysts break apart into single oocytes that become surrounded by pregranulosa cells to form primordial follicles. During the process of cyst breakdown, a subset of cells in each cyst undergoes cell death with only one-third of the initial number of oocytes surviving to form primordial follicles. The mechanisms that control cyst breakdown, oocyte survival, and follicle assembly are currently under investigation. This review describes the mechanisms that have been implicated in the control of primordial follicle formation, which include programmed cell death regulation, growth factor and other signaling pathways, regulation by transcription factors and hormones, meiotic progression, and changes in cell adhesion. Elucidation of mechanisms leading to formation of the primordial follicle pool will help research efforts in ovarian biology and improve treatments of female infertility, premature ovarian failure, and reproductive cancers.

show abstract

“…oocyte vs theca) cell types have diverse biological responses within the granulosa layer, the latter of which are mediated by the various type I receptors (ALKs) and intracellular Smad response proteins (Massague 1998, Mazerbourg et al 2004. Whether GDF-9 and BMP-4 have stimulatory effects on preantral follicular growth in cattle as they do in rodents (Vitt et al 2000b, Nilsson & Skinner 2002, Latham et al 2004, Wang & Roy 2004 and humans (Hreinsson et al 2002) awaits further elucidation. Also, whether GDF-9 plays a role in the regulation of ovulation rate in cattle, as recently demonstrated for sheep (Hanrahan et al 2004, Juengel et al 2004, will require additional research.…”

Section: ·05 4·3 17mentioning

confidence: 99%

Growth differentiation factor-9 has divergent effects on proliferation and steroidogenesis of bovine granulosa cells

Spicer

Aad

Allen

et al. 2006

Journal of Endocrinology

View full text Add to dashboard Cite

In addition to gonadotropins, steroidogenesis and proliferation of granulosa cells during follicular development are controlled by a number of intraovarian factors including growth differentiation factor-9 (GDF-9), bone morphogenetic protein-4 (BMP-4), and IGF-I. The objective of this study was to determine the effect of GDF-9 and BMP-4 and their interaction with IGF-I and FSH on ovarian granulosa cell function in cattle. Granulosa cells from small (1-5 mm) and large (8-22 mm) follicles were collected from bovine ovaries and cultured for 48 h in medium containing 10% fetal calf serum and then treated with various hormones in serum-free medium for an additional 48 h. We evaluated the effects of GDF-9 (150-600 ng/ml) and BMP-4 (30 ng/ml) during a 2-day exposure on hormone-induced steroidogenesis and cell proliferation. In FSH plus IGF-I-treated granulosa cells obtained from small follicles, 300 ng/ml GDF-9 reduced (P<0·05) progesterone production by 15% and 600 ng/ml GDF-9 completely blocked (P<0·01) the IGF-I-induced increase in progesterone production. In comparison, 300 and 600 ng/ml GDF-9 decreased (P<0·05) estradiol production by 27% and 71% respectively, whereas 150 ng/ml GDF-9 was without effect (P>0·10). Treatment with 600 ng/ml GDF-9 increased (P<0·05) numbers (by 28%) of granulosa cells from small follicles. In the same cells treated with FSH but not IGF-I, co-treatment with 600 ng/ml GDF-9 decreased (P<0·05) progesterone production (by 28%), increased (P<0·05) cell numbers (by 60%), and had no effect (P>0·10) on estradiol production. In FSH plus IGF-I-treated granulosa cells obtained from large follicles, GDF-9 caused a dose-dependent decrease (P<0·05) in IGF-I-induced progesterone (by 13-48%) and estradiol (by 20-51%) production. In contrast, GDF-9 increased basal and IGF-I-induced granulosa cell numbers by over 2-fold. Furthermore, treatment with BMP-4 also inhibited (P<0·05) steroidogenesis by 27-42% but had no effect on cell numbers. To elucidate downstream signaling pathways, granulosa cells from small follicles were transfected with similar to mothers against decapentaplegics (Smad) binding element (CAGA)-or BMP response element (BRE)-promoter reporter constructs. Treatment with GDF-9 (but not BMP-4) activated the Smad3-induced CAGA promoter activity, whereas BMP-4 (but not GDF-9) activated the Smad1/5/8-induced BRE promoter activity. We have concluded that bovine granulosa cells are targets of both GDF-9 and BMP-4, and that oocyte-derived GDF-9 may simultaneously promote granulosa cell proliferation and prevent premature differentiation of the granulosa cells during growth of follicles, whereas theca-derived BMP-4 may also prevent premature follicular differentiation.

show abstract

Growth Differentiation Factor-9 and Stem Cell Factor Promote Primordial Follicle Formation in the Hamster: Modulation by Follicle-Stimulating Hormone1

Cited by 106 publications

References 17 publications

In vitro culture of sheep lamb ovarian cortical tissue in a sequential culture medium

In vitro culture of sheep lamb ovarian cortical tissue in a sequential culture medium

Follicular assembly: mechanisms of action

Growth differentiation factor-9 has divergent effects on proliferation and steroidogenesis of bovine granulosa cells

Contact Info

Product

Resources

About