Growth Differentiation Factor 5 Accelerates Wound Closure and Improves Skin Quality During Repair of Full-Thickness Skin Defects

Schiefer, Jennifer Lynn; Held, Manuel; Fuchs, Paul Christian; Demir, Erhan; Plöger, Frank; Schaller, Hans-Eberhard; Rahmanian-Schwarz, Afshin

doi:10.1097/01.asw.0000515078.69041.3c

Cited by 10 publications

(17 citation statements)

References 24 publications

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“…Other studies had reported that GDF-5 promote cell proliferation [13], in this study, we found that GDF-5 can directly increase the number of EpSCs in vitro. We detected the effect of GDF-5 on EpSCs when the concentration of exogenous GDF-5 changed from 0 to 1000 ng/ml by CCK-8 assay at 12 hours, 24 hours, 48 hours and 72 hours.…”

Section: Discussionsupporting

confidence: 74%

“…At present, the effect of GDF-5 on wound healing has been reported [13,15]. However, its speci c mechanism for wound repairing is still unclear.…”

Section: Discussionmentioning

confidence: 99%

“…Syed H.E and colleagues also discovered that GDF5-induced p38-MAPK signaling in broblasts regulate cardiac repairing after myocardial infarction [15]. The predecessors conducted a preliminary study effect of GDF-5 on the wound repair [13], so we speculate that the promotion of GDF-5 may promote the proliferation of EpSCs, but the speci c proliferation mechanism of EpSCs promoted by GDF-5 has not been reported. Recent studies have found that FOX (Fork head box) and cyclin are involved in the promotion of cell proliferation by BMP [19].…”

Section: Introductionmentioning

confidence: 88%

“…The physiological state of EpSCs is affected by different signaling pathways, including MAPK (mitogenactivated protein kinase) [8,9], Wnt (wingless) [10,11] and TGF-β (transforming growth factor-beta) [12] signaling, etc. BMP (bone morphogenetic protein) belongs to the TGF-β family and can stimulate cell proliferation [13].…”

Section: Introductionmentioning

confidence: 99%

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GDF-5 promotes Epidermal Stem Cells proliferation via Foxg1-cyclin D1 signaling

Zhao

Bian²,

Wang³

et al. 2020

Preprint

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Objective: Epidermal stem cells (EpSCs) can self-renew and are responsible for the long-term maintenance of the skin, it also plays a critical role in wound re-epithelization, but the mechanism underlying EpSCs proliferation is unclear. Here, we studied the effects of GDF-5 on mouse EpSCs proliferation mechanism in wound healing. Methods: Firstly, the effects of GDF-5 on EpSCs proliferation was tested by using CCK8 reagent and PCNA expression was analysed by western blotting. Secondly, we screened genes that promote EpSCs proliferation in the FOX family and cyclin by qPCR, and further analysed the protein expression level of the selected genes by Western blotting. Thirdly, siRNA plasmids and pAdEasy adenovirus were transfected or infected, respectively, into mouse EpSCs to detect the effect of target genes on GDF-5 induced cell proliferation. Furthermore, a deep partial thickness burn mouse model was used in which GDF-5 induced EpSCs proliferation was detected by immunohistochemical. Finally, the relationships target genes were analysed by qPCR, immunoblotting and dual luciferase reporter gene detection. Results: We discovered that 100 ng/ml recombinant mouse GDF-5 was the optimal concentration for promoting mouse EpSCs proliferation. Through preliminary screening by qPCR, we found that Foxg1 and cyclin D1 could be the downstream molecules of GDF-5, and the results were confirmed by Western blotting. And the effect of GDF-5 on mouse EpSCs proliferation was regulated by Foxg1/cyclin D1 in vitro and in vivo. Besides, GDF-5 induced transcription of cyclin D1 was regulated by Foxg1-mediated cyclin D1 promoter activity.Conclusion: This paper showed that GDF-5 promotes mouse EpSCs proliferation via Foxg1-cyclin D1 signal pathway. It is suggested that GDF-5 may be a new approach to yield EpSCs for wound healing.

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Section: Discussionsupporting

confidence: 74%

“…At present, the effect of GDF-5 on wound healing has been reported [13,15]. However, its speci c mechanism for wound repairing is still unclear.…”

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 88%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

GDF-5 promotes Epidermal Stem Cells proliferation via Foxg1-cyclin D1 signaling

Zhao

Bian²,

Wang³

et al. 2020

Preprint

View full text Add to dashboard Cite

show abstract

“…The physiological state of EpSCs can be affected by different signaling pathways, including MAPK (mitogen-activated protein kinase) [8,9], Wnt (wingless) [10,11], and TGF-β (transforming growth factor-beta) [12] signaling. BMP (bone morphogenetic protein) belongs to the TGF-β family and can stimulate cell proliferation [13].…”

Section: Introductionmentioning

confidence: 99%

GDF-5 promotes epidermal stem cells proliferation via Foxg1-cyclin D1 signaling

et al. 2021

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Objective Epidermal stem cells (EpSCs) can self-renew, which are responsible for the long-term maintenance of the skin, and it also plays a critical role in wound re-epithelization, but the mechanism underlying EpSCs proliferation is unclear. GDF-5, also known as BMP-14, is a member of the BMP family and can be used as a self-renewal supporter. Here, we studied the effects of GDF-5 on mouse EpSCs proliferation mechanism in wound healing. Methods Firstly, the effects of GDF-5 on EpSCs proliferation was tested by using CCK8 reagent and PCNA expression was analyzed by Western blotting. Secondly, we screened genes that promote EpSCs proliferation in the FOX and cyclin family by qPCR, and then the protein expression level of the selected genes was further analyzed by Western blotting. Thirdly, siRNA plasmids and pAdEasy adenovirus were transfected or infected, respectively, into mouse EpSCs to detect the effect of target genes on GDF-5-induced cell proliferation. Furthermore, we injected GDF-5 to a deep partial thickness burn mouse model for finding out whether EpSCs proliferation can be detected by immunohistochemical. Finally, the relevant target genes were analyzed by qPCR, immunoblotting, and dual-luciferase reporter gene detection. Results We discovered that 100 ng/ml recombinant mouse GDF-5 was the optimal concentration for promoting mouse EpSCs proliferation. Through preliminary screened by qPCR, we found that Foxg1 and cyclin D1 could be the downstream molecules of GDF-5, and the results were confirmed by Western blotting. And the effect of GDF-5 on mouse EpSCs proliferation was adjusted by Foxg1/cyclin D1 in vitro and in vivo. Besides, GDF-5-induced transcription of cyclin D1 was regulated by Foxg1-mediated cyclin D1 promoter activity. Conclusion This paper showed that GDF-5 promotes mouse EpSCs proliferation via Foxg1-cyclin D1 signal pathway. It is suggested that GDF-5 may be a new approach to make EpSCs proliferation which can be used in wound healing.

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