Growth, development and steviol glycosides content in the relation to the photosynthetic activity of several Stevia rebaudiana Bertoni strains cultivated under temperate climate conditions

Libik-Konieczny, Marta; Capecka, E.; Kąkol, Edyta; Dziurka, Michał; Grabowska-Joachimiak, Aleksandra; Śliwińska, Elwira; Pistelli, Laura

doi:10.1016/j.scienta.2018.02.015

Cited by 20 publications

(9 citation statements)

References 40 publications

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“…SGs play important role in the adaptation of plants to stress environments via alleviating stress associated effects [ 44 , 45 ]. Libik-Konieczny et al [ 46 ] presented a hypothesis that steviol glycosides might function in the protection of photosynthetic apparatus against adverse environmental conditions. A standard literature states that the primary function of polyphenols is also to act as UV protection agents in plants [ 47 , 48 ].…”

Section: Discussionmentioning

confidence: 99%

“…Nevertheless, the tendency found in this study was reproducible in similar study using other type of cold plasma (dielectric barrier discharge plasma) treatment and different varieties of stevia (unpublished data). Libik-Konieczny et al [ 46 ], however, demonstrated the opposite reaction of stevia to adverse environmental conditions, i.e., decrease in SGs amount, but similar tendency of inversely proportional changes in SGs and TPC—the climatic stress resulted in lower SGs production but higher TPC. There is little information on the crosstalk of the SG biosynthesis pathways with biosynthetic pathways of phenolic compounds and flavonoids.…”

Section: Discussionmentioning

confidence: 99%

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The Potential of Cold Plasma and Electromagnetic Field as Stimulators of Natural Sweeteners Biosynthesis in Stevia rebaudiana Bertoni

Judickaitė

Lyushkevich

Филатова

et al. 2022

Plants

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Stevioside (Stev) and rebaudioside A (RebA) are the most abundant steviol glycosides (SGs) responsible for the sweetness of Stevia rabaudiana Bertoni. As compared to Stev, RebA has a higher sweetening potency, better taste and therefore is the most preferred component of the stevia leaf extracts. The aim of this study was to determine the effect of pre-sowing seed treatment with abiotic stressors cold plasma (CP) and electromagnetic field (EMF) on the amount and ratio of RebA and Stev in the leaves of stevia. Additionally, the effect on total phenolic content, flavonoid content and antioxidant activity was investigated. Seeds were treated 5 and 7 min with cold plasma (CP5 and CP7 groups) and 10 min with electromagnetic field (EMF10 group) six days before sowing. The germination tests in vitro demonstrated that all treatments slightly increased germination rate and percentage. HPLC analysis revealed that CP and EMF had strong stimulating effect on SGs accumulation. All treatments increased RebA concentration approximately 1.6-fold; however, the ratio of RebA/Stev decreased from 8.5 in the control to 1.9, 2.5 and 1.1 in CP5, CP7 and EMF10 groups respectively, since the concentration of Stev increased more than RebA, 7.1, 4.6 and 11.0-fold, respectively, compared to control. However, treatments had opposite effect on total phenolic content, flavonoid content, and antioxidant activity. We have demonstrated for the first time that short time pre-sowing treatment of stevia seeds with CP and EMF can be a powerful tool for the enhancement of biosynthesis of RebA and Stev, however it can have negative impact on the content of other secondary metabolites.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

The Potential of Cold Plasma and Electromagnetic Field as Stimulators of Natural Sweeteners Biosynthesis in Stevia rebaudiana Bertoni

Judickaitė

Lyushkevich

Филатова

et al. 2022

Plants

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“…SGs are synthetized via the plastid localized methylerythritol 4-phosphate (MEP) pathway from the precursors glyceraldehyde 3-phosphate (G3P) and pyruvate [ 4 , 5 ]. Since G3P derives from photosynthesis and the MEP pathway also supports the synthesis of photosynthetic pigments (chlorophylls and carotenoids), SG formation is tightly associated with photosynthetic activity and some SGs might contribute to the protection of the photosynthetic apparatus against environmental stresses [ 6 ].…”

Section: Introductionmentioning

confidence: 99%

The Effect of Foliar Selenium (Se) Treatment on Growth, Photosynthesis, and Oxidative-Nitrosative Signalling of Stevia rebaudiana Leaves

et al. 2021

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Selenium (Se) enrichment of Stevia rebaudiana Bertoni can serve a dual purpose, on the one hand to increase plant biomass and stress tolerance and on the other hand to produce Se fortified plant-based food. Foliar Se spraying (0, 6, 8, 10 mg/L selenate, 14 days) of Stevia plantlets resulted in slightly decreased stevioside and rebaudioside A concentrations, and it also caused significant increment in stem elongation, leaf number, and Se content, suggesting that foliar Se supplementation can be used as a biofortifying approach. Furthermore, Se slightly limited photosynthetic CO2 assimilation (AN, gsw, Ci/Ca), but exerted no significant effect on chlorophyll, carotenoid contents and on parameters associated with photosystem II (PSII) activity (FV/FM, F0, Y(NO)), indicating that Se causes no photodamage in PSII. Further results indicate that Se is able to activate PSI-cyclic electron flow independent protection mechanisms of the photosynthetic apparatus of Stevia plants. The applied Se activated superoxide dismutase (SOD) isoenzymes (MnSOD1, FeSOD1, FeSOD2, Cu/ZnSOD1, Cu/ZnSOD2) and down-regulated NADPH oxidase suggesting the Se-induced limitation of superoxide anion levels and consequent oxidative signalling in Stevia leaves. Additionally, the decrease in S-nitrosoglutathione reductase protein abundance and the intensification of protein tyrosine nitration indicate Se-triggered nitrosative signalling. Collectively, these results suggest that Se supplementation alters Stevia shoot morphology without significantly affecting biomass yield and photosynthesis, but increasing Se content and performing antioxidant effects, which indicates that foliar application of Se may be a promising method in Stevia cultivation.

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“…Brazil, the United States and Argentina are also counted among important S. rebaudiana producers (Gantait et al, 2018). However, some European studies show that it is also possible to cultivate stevia in temperate conditions as a perennial plant in southern countries and as an annual plant in northern countries (Andolfi et al, 2006; Hastoy, Cosson, et al, 2019; Libik‐Konieczny et al, 2018; Munz et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

“…Most studies are based on phenotypic data, but the lack of a common tool to describe these phenological stages complicates comparison and knowledge transfer. Indeed, in most studies harvest times are described using general terms such as vegetative stage, flower budding stage, flowering stage or just dates, with each term representing a wide range of phenological stages (Barbet‐Massin et al, 2016; Libik‐Konieczny et al, 2018; Moraes et al, 2013; Reis et al, 2015; Tavarini & Angelini, 2013).…”

Section: Introductionmentioning

confidence: 99%

Phenological growth stages of stevia (Stevia rebaudiana Bertoni) according to the Biologische Bundesanstalt Bundessortenamt and Chemical Industry (BBCH) scale

Bihan

Cosson

Schurdi‐Levraud

2020

Annals of Applied Biology

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Stevia (Stevia rebaudiana Bertoni) is a perennial crop belonging to the Asteraceae family. Its main agronomic and industrial interest is the accumulation in its leaves of steviol glycosides (SG), natural acaloric sweeteners which are 300 times sweeter than sucrose. In order to expand phenotyping knowledge on this emerging crop, researchers and breeders need a universal scale to describe the development of this plant. The objective of this study was to describe the phenological growth stages of stevia through the use of the Biologische Bundesanstalt Bundessortenamt and Chemical Industry (BBCH) scale to provide a standard scale for stevia grown under temperate conditions of production and climate. The observation of phenological stages was performed on genetic resources and validated on stevia progenies grown in field conditions. The 10 phenological stages, namely germination, bud development, foliar development, side shoots appearance, stems elongation, development of harvestable part of the crop, inflorescence emergence, flowering, development of fruit, seeds ripening and senescence, were used to describe the stevia, supplemented with pictures. The chronology and duration of each stage were also observed and described according to the accumulation of degree days during the growing season. This study presents the first BBCH scale established for S. rebaudiana cultivated in temperate conditions and a chronology of phenological stages based on the accumulation of thermal time, through growing degree day calculation (°C/day). This work provides a tool that can be widely used by farmers, breeders and researchers leading to better agronomic management and scientific communication.

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Growth, development and steviol glycosides content in the relation to the photosynthetic activity of several Stevia rebaudiana Bertoni strains cultivated under temperate climate conditions

Cited by 20 publications

References 40 publications

The Potential of Cold Plasma and Electromagnetic Field as Stimulators of Natural Sweeteners Biosynthesis in Stevia rebaudiana Bertoni

The Potential of Cold Plasma and Electromagnetic Field as Stimulators of Natural Sweeteners Biosynthesis in Stevia rebaudiana Bertoni

The Effect of Foliar Selenium (Se) Treatment on Growth, Photosynthesis, and Oxidative-Nitrosative Signalling of Stevia rebaudiana Leaves

Phenological growth stages of stevia (Stevia rebaudiana Bertoni) according to the Biologische Bundesanstalt Bundessortenamt and Chemical Industry (BBCH) scale

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