Growth, death, and photobiology of dinoflagellates (Dinophyceae) under bacterial-algicide control

Tilney, Charles L.; Pokrzywinski, Kaytee L.; Coyne, Kathryn J.; Warner, Mark E.

doi:10.1007/s10811-014-0248-z

Cited by 30 publications

(44 citation statements)

References 57 publications

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“…However, while the algicide has been effective against all dinoflagellates tested to date some species are more susceptible than others. This and other studies1821 have shown that the algicide may be the best at controlling athecate dinoflagellates during logarithmic growth stages and therefore may be best applied during the early stages of a bloom or as a preventive measure in problematic areas. However, further purification and structural elucidation of the active compound(s) need to be conducted before IRI-160AA can be applied in the natural environment.…”

Section: Discussionmentioning

confidence: 52%

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Cell cycle arrest and biochemical changes accompanying cell death in harmful dinoflagellates following exposure to bacterial algicide IRI-160AA

Pokrzywinski

Tilney

Warner

et al. 2017

Sci Rep

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Bacteria may play a role in regulating harmful algal blooms, but little is known about the biochemical and physiological changes associated with cell death induced by algicidal bacteria. Previous work characterized an algicidal exudate (IRI-160AA) produced by Shewanella sp. IRI-160 that is effective against dinoflagellates, while having little to no effect on other phytoplankton species in laboratory culture experiments. The objective of this study was to evaluate biochemical changes associated with cell death and impacts on the cell cycle in three dinoflagellate species (Prorocentrum minimum, Karlodinium veneficum and Gyrodinium instriatum) after exposure to IRI-160AA. In this study, IRI-160AA induced cell cycle arrest in all dinoflagellates examined. Several indicators for programmed cell death (PCD) that are often observed in phytoplankton in response to a variety of stressors were also evaluated. Cell death was accompanied by significant increases in DNA degradation, intra- and extracellular ROS concentrations and DEVDase (caspase-3 like) protease activity, which have been associated with PCD in other phytoplankton species. Overall, results of this investigation provide strong evidence that treatment with the bacterial algicide, IRI-160AA results in cell cycle arrest and induces biochemical changes consistent with stress-related cell death responses observed in other phytoplankton.

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Section: Discussionmentioning

confidence: 52%

“…The differences in chloroplast structure, surface area to volume ratio, degree of cell covering and toxin status may directly impact the susceptibility of each species to the algicide. For example Tilney et al 21. found an inverse relationship between algicidal activity and degree of plasma membrane exposure (presence/absence of theca).…”

mentioning

confidence: 99%

Cell cycle arrest and biochemical changes accompanying cell death in harmful dinoflagellates following exposure to bacterial algicide IRI-160AA

Pokrzywinski

Tilney

Warner

et al. 2017

Sci Rep

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“…Panels a and b adapted from Berman-Frank et al (2004); panels c and d adapted from Berman-Frank et al (2007); panels e and f adapted from Bar-Zeev et al (2013); panels g-k adapted from Vardi et al (2012); panels l-s adapted from Kahl et al (2008). bioactive compound (IRI-160AA) by Shewanella sp. IRI-160 induces algicidal activity in a broad range of dinoflagellates but has little or no effect on chlorophytes and cryptophytes (Pokrzywinski et al 2012, Tilney et al 2014, with most dinoflagellates exhibiting PSII inhibition and varying degrees of cellular mortality and loss of membrane integrity. Subsequent work with IRI-160AA-treated dinoflagellate cells verified PCD induction via significant increases in hydrogen peroxide, intracellular ROS, caspase 3-like activity, and inversion of phosphatidylserine in cell membranes (K.L.…”

Section: Emerging Roles Of Signaling Allelopathy and Cell-cell Intementioning

confidence: 99%

The Molecular Ecophysiology of Programmed Cell Death in Marine Phytoplankton

Bidle

2015

Annu. Rev. Mar. Sci.

131

143

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Planktonic, prokaryotic, and eukaryotic photoautotrophs (phytoplankton) share a diverse and ancient evolutionary history, during which time they have played key roles in regulating marine food webs, biogeochemical cycles, and Earth's climate. Because phytoplankton represent the basis of marine ecosystems, the manner in which they die critically determines the flow and fate of photosynthetically fixed organic matter (and associated elements), ultimately constraining upper-ocean biogeochemistry. Programmed cell death (PCD) and associated pathway genes, which are triggered by a variety of nutrient stressors and are employed by parasitic viruses, play an integral role in determining the cell fate of diverse photoautotrophs in the modern ocean. Indeed, these multifaceted death pathways continue to shape the success and evolutionary trajectory of diverse phytoplankton lineages at sea. Research over the past two decades has employed physiological, biochemical, and genetic techniques to provide a novel, comprehensive, mechanistic understanding of the factors controlling this key process. Here, I discuss the current understanding of the genetics, activation, and regulation of PCD pathways in marine model systems; how PCD evolved in unicellular photoautotrophs; how it mechanistically interfaces with viral infection pathways; how stress signals are sensed and transduced into cellular responses; and how novel molecular and biochemical tools are revealing the impact of PCD genes on the fate of natural phytoplankton assemblages.

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“…According to the BWMC-Guideline 8, the evaluation of the treatment efficacy is based on the determination of living organisms by widely accepted standard methods (Gollasch et al 2012;Cullen and MacIntyre 2016), such as vital fluorescence stains detected using flow cytometry or fluorescence microscopy, ATP content, photosynthetic activity, or motility assessment (Hessen et al 1997;Sato et al 2004;Gavand et al 2007;Gallo-Villanueva et al 2011;Steinberg et al 2011Steinberg et al , 2012Fan et al 2013;Martínez et al 2013;Stehouwer et al 2013;Adams et al 2014;First and Drake 2014;Tilney et al 2014;Feng et al 2015;Gollasch et al 2015;Olsen et al 2015;van Slooten et al 2015;Wright et al 2015). However, the application of UV treatment does not cause immediate changes in the concentration of living organisms, although their growth curves become different over the time, depending on the applied UV dose (Tao et al 2010;Gorokhova et al 2012;Martínez et al 2012).…”

Section: Introductionmentioning

confidence: 99%

Evaluation of ultraviolet disinfection of microalgae by growth modeling: application to ballast water treatment

et al. 2016

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Ultraviolet disinfection is a frequent option for eliminating viable organisms in ballast water to fulfill international and national regulations. The objective of this work is to evaluate the reduction of microalgae able to reproduce after UV irradiation, based on their growth features. A monoculture of microalgae Tisochrysis lutea was irradiated with different ultraviolet doses (UV-C 254 nm) by a flow-through reactor. A replicate of each treated sample was held in the dark for 5 days simulating a treatment during the ballasting; another replicate was incubated directly under the light, corresponding to the treatment application during de-ballasting. Periodic measurements of cell density were taken in order to obtain the corresponding growth curves. Irradiated samples depicted a regrowth following a logistic curve in concordance with the applied UV dose. By modeling these curves, it is possible to obtain the initial concentration of organisms able to reproduce for each applied UV dose, thus obtaining the dose-survival profiles, needed to determine the disinfection kinetics. These dose-survival profiles enable detection of a synergic effect between the ultraviolet irradiation and a subsequent dark period; in this sense, the UV dose applied during the ballasting operation and subsequent dark storage exerts a strong influence on microalgae survival. The proposed methodology, based on growth modeling, established a framework for comparing the UV disinfection by different devices and technologies on target organisms. This procedure may also assist the understanding of the evolution of treated organisms in more complex assemblages such as those that exist in natural ballast water.

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Growth, death, and photobiology of dinoflagellates (Dinophyceae) under bacterial-algicide control

Cited by 30 publications

References 57 publications

Cell cycle arrest and biochemical changes accompanying cell death in harmful dinoflagellates following exposure to bacterial algicide IRI-160AA

Cell cycle arrest and biochemical changes accompanying cell death in harmful dinoflagellates following exposure to bacterial algicide IRI-160AA

The Molecular Ecophysiology of Programmed Cell Death in Marine Phytoplankton

Evaluation of ultraviolet disinfection of microalgae by growth modeling: application to ballast water treatment

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