Growth control in the<i>Drosophila</i>eye disc by the cytokine Unpaired

Vollmer, Jannik; Fried, Patrick; Aguilar-Hidalgo, Daniel; Sánchez-Aragón, Máximo; Iannini, Antonella; Casares, Fernando; Iber, Dagmar

doi:10.1242/dev.141309

Cited by 19 publications

(27 citation statements)

References 27 publications

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“…The R-cell developmental defects suggest a role for these genes in promoting cell proliferation and differentiation in the eye disc. Indeed, dome, EGFR and kuz have been shown previously to function in key signaling pathways for regulating cell growth and differentiation [21]. For instance, dome encodes for a transmembrane receptor for the cytokine-like ligand Upd, and functions in activating the intracellular JAK/STAT pathway [22].…”

Section: Discussionmentioning

confidence: 99%

An RNAi screen for secreted factors and cell-surface players in coordinating neuron and glia development in Drosophila

2020

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The establishment of the functional nervous system requires coordinated development of neurons and glia in the embryo. Our understanding of underlying molecular and cellular mechanisms, however, remains limited. The developing Drosophila visual system is an excellent model for understanding the developmental control of the nervous system. By performing a systematic transgenic RNAi screen, we investigated the requirements of secreted proteins and cell-surface receptors for the development of photoreceptor neurons (R cells) and wrapping glia (WG) in the Drosophila visual system. From the screen, we identified seven genes whose knockdown disrupted the development of R cells and/or WG, including amalgam (ama), domeless (dome), epidermal growth factor receptor (EGFR), kuzbanian (kuz), N-Cadherin (CadN), neuroglian (nrg), and shotgun (shg). Cell-type-specific analysis revealed that ama is required in the developing eye disc for promoting cell proliferation and differentiation, which is essential for the migration of glia in the optic stalk. Our results also suggest that nrg functions in both eye disc and WG for coordinating R-cell and WG development.

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Section: Discussionmentioning

confidence: 99%

An RNAi screen for secreted factors and cell-surface players in coordinating neuron and glia development in Drosophila

2020

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“…in a growing tissue) and/or across a population of individuals. (Barkai & Shilo, 2013;Vollmer et al, 2017) Somitogenesis/Somites Somites are precursors of the repetitive body segments consisting of vertebrae and their associated muscles, occurring in vertebrates. Somitogenesis is the embryonic developmental process that gives rise to somites, which are formed from the PSM.…”

Section: Scalingmentioning

confidence: 99%

“…Scaling is a universal biological phenomenon: structures and patterns form proportionally to a reference size, such as the body size or the size of the patterned domain. This phenomenon is still not understood, with intriguing open questions such as (Barkai & Shilo, 2013;Vollmer et al, 2017): How is tissue size measured? How is growth of developing structures terminated in various systems?…”

Section: A3 Ex Vivo Scaling Of the Oscillation Phase Gradientmentioning

confidence: 99%

Travelling waves in somitogenesis: Collective cellular properties emerge from time-delayed juxtacrine oscillation coupling

Tomka

Iber

Boareto

2018

Progress in Biophysics and Molecular Biology

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The sculpturing of the vertebrate body plan into segments begins with the sequential formation of somites in the presomitic mesoderm (PSM). The rhythmicity of this process is controlled by travelling waves of gene expression. These kinetic waves emerge from coupled cellular oscillators and sweep across the PSM. In zebrafish, the oscillations are driven by autorepression of her genes and are synchronized via Notch signalling. Mathematical modelling has played an important role in explaining how collective properties emerge from the molecular interactions. Increasingly more quantitative experimental data permits the validation of those mathematical models, yet leads to increasingly more complex model formulations that hamper an intuitive understanding of the underlying mechanisms. Here, we review previous efforts, and design a mechanistic model of the her1 oscillator, which represents the experimentally viable her7;hes6 double mutant. This genetically simplified system is ideally suited to conceptually recapitulate oscillatory entrainment and travelling wave formation, and to highlight open questions. It shows that three key parameters, the autorepression delay, the juxtacrine coupling delay, and the coupling strength, are sufficient to understand the emergence of the collective period, the collective amplitude, and the synchronization of neighbouring Her1 oscillators. Moreover, two spatiotemporal time delay gradients, in the autorepression and in the juxtacrine signalling, are required to explain the collective oscillatory dynamics and synchrony of PSM cells. The highlighted developmental principles likely apply more generally to other developmental processes, including neurogenesis and angiogenesis.

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“…Picture and legend in ( b ) were adapted and reproduced with permission from Fried et al [ 85 ] (Copyright © 2016 Public Library of Science). The pictures in ( c–e ) and the legend for ( e ) were adapted from Vollmer et al [ 86 ]. Reprinted with permission from The Company of Biologists (UK).…”

Section: Models For Controlling Growth Terminationmentioning

confidence: 99%

“…Also, Upd is sufficiently long-lived (approx. 60 h) that its concentration is mainly reduced by growth-dependent dilution rather than by protein turn-over [ 86 ]. Biochemical studies further show that the intracellular JAK/STAT pathway responds about linearly to the Upd levels [ 92 , 93 ].…”

Section: Models For Controlling Growth Terminationmentioning

confidence: 99%

Growth and size control during development

2017

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The size and shape of organs are characteristic for each species. Even when organisms develop to different sizes due to varying environmental conditions, such as nutrition, organ size follows species-specific rules of proportionality to the rest of the body, a phenomenon referred to as allometry. Therefore, for a given environment, organs stop growth at a predictable size set by the species's genotype. How do organs stop growth? How can related species give rise to organs of strikingly different size? No definitive answer has been given to date. One of the major models for the studies of growth termination is the vinegar fly Drosophila melanogaster. Therefore, this review will focus mostly on work carried out in Drosophila to try to tease apart potential mechanisms and identify routes for further investigation. One general rule, found across the animal kingdom, is that the rate of growth declines with developmental time. Therefore, answers to the problem of growth termination should explain this seemingly universal fact. In addition, growth termination is intimately related to the problems of robustness (i.e. precision) and plasticity in organ size, symmetric and asymmetric organ development, and of how the 'target' size depends on extrinsic, environmental factors.

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Growth control in theDrosophilaeye disc by the cytokine Unpaired

Cited by 19 publications

References 27 publications

An RNAi screen for secreted factors and cell-surface players in coordinating neuron and glia development in Drosophila

An RNAi screen for secreted factors and cell-surface players in coordinating neuron and glia development in Drosophila

Travelling waves in somitogenesis: Collective cellular properties emerge from time-delayed juxtacrine oscillation coupling

Growth and size control during development

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