Growth cone interactions with purified cell and substrate adhesion molecules visualized by interference reflection microscopy

Drazba, Judith A.; Liljelund, Patricia; Smith, C G; Payne, Ross; Lemmon, Vance

doi:10.1016/s0165-3806(97)00041-2

Cited by 17 publications

(14 citation statements)

References 49 publications

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“…Classic in vitro studies demonstrated that growth cones with flattened, lamellar morphologies were strongly attached to the underlying substrate (Letourneau, 1975). Subsequent work indicated that growth cones were large, flat, more adherent, and slower growing when extending on cell adhesion molecules and were small, filopodial, less adherent, and faster growing when extending on extracellular matrix proteins Lemmon et al, 1992;Drazba et al, 1997). In the present studies, nearly 90% of ORN growth cones stopped advancing after encountering SZ and NP glial cells.…”

Section: Growth Cone Responses To Sz and Np Glial Cellssupporting

confidence: 47%

In vitro analyses of interactions between olfactory receptor growth cones and glial cells that mediate axon sorting and glomerulus formation

Tucker

Oland

Tolbert

2004

J of Comparative Neurology

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During development, the axons of olfactory receptor neurons project to the CNS and converge on glomerular targets. For vertebrate and invertebrate olfactory systems, neuron-glia interactions have been hypothesized to regulate the sorting and targeting of olfactory receptor axons and the development of glomeruli. In the moth Manduca sexta, glial reduction experiments have directly implicated two types of central olfactory glia, the sorting zone- and neuropil-associated glia, in key events in olfactory development, including axon sorting and glomerulus stabilization. By using cocultures containing central olfactory glial cells and explants of olfactory receptor epithelium, we show that olfactory receptor growth cones elaborate extensively and cease advancement following contact with sorting zone- and neuropil-associated glial cells. These effects on growth cone behavior were specific to central olfactory glia; peripheral glial cells of the olfactory nerve failed to elicit similar responses in olfactory receptor growth cones. We propose that sorting zone- and neuropil-associated glial cells similarly modify axon behavior in vitro by altering the adhesive properties and cytoskeleton of olfactory receptor growth cones and that these in vitro changes may underlie functionally relevant changes in growth cone behavior in vivo.

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Section: Growth Cone Responses To Sz and Np Glial Cellssupporting

confidence: 47%

In vitro analyses of interactions between olfactory receptor growth cones and glial cells that mediate axon sorting and glomerulus formation

Tucker

Oland

Tolbert

2004

J of Comparative Neurology

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“…[64] A few studies have also addressed the contact between a neuronal growth cone and substrate coated with various proteins involved in adhesion or migration. [65] …”

Section: Tight Adhesionmentioning

confidence: 99%

Quantitative Reflection Interference Contrast Microscopy (RICM) in Soft Matter and Cell Adhesion

2009

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Adhesion can be quantified by measuring the distance between the interacting surfaces. Reflection interference contrast microscopy (RICM), with its ability to measure inter-surface distances under water with nanometric precision and milliseconds time resolution, is ideally suited to studying the dynamics of adhesion in soft systems. Recent technical developments, which include innovative image analysis and the use of multi-coloured illumination, have led to renewed interest in this technique. Unambiguous quantitative measurements have been achieved for colloidal beads and model membranes, thus revealing new insights and applications. Quantification of data from cells shows exciting prospects. Herein, we review the basic principles and recent developments of RICM applied to studies of dynamical adhesion processes in soft matter and cell biology and provide practical hints to potential users.

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“…Also, it should be mentioned that it has recently been demonstrated that Cadherin-11 and the Ig family member, L1, which is a prominent inducer of neurite outgrowth [3], also interact with FGFR and induce neurite outgrowth [48,101], suggesting that a number of different CAMs may signal via interactions with FGFR. NCAM and N-cadherin (and other CAMs) are dynamically expressed in the growing tip of neurites [102][103][104] and the strength of adhesion at the neurite tip is highly dynamic. A relationship has been suggested to exist between growth rate and adhesion stability, such that tight adhesion correlates with slow growth and loose adhesion correlates with fast growth [102].…”

Section: Ncam-vs N-cadherin-mediated Neurite Outgrowthmentioning

confidence: 99%

“…NCAM and N-cadherin (and other CAMs) are dynamically expressed in the growing tip of neurites [102][103][104] and the strength of adhesion at the neurite tip is highly dynamic. A relationship has been suggested to exist between growth rate and adhesion stability, such that tight adhesion correlates with slow growth and loose adhesion correlates with fast growth [102]. Although this could be interpreted simply as a mechanical function of CAMs (e.g., tight adhesion prevents the tip from elongating), research over the past decade has established that many CAMs function as sensors of the environment that, upon extracellular cues (such as loose adhesion), induce intracellular signaling to promote neurite growth.…”

Section: Ncam-vs N-cadherin-mediated Neurite Outgrowthmentioning

confidence: 99%

Signaling mechanisms of neurite outgrowth induced by the cell adhesion molecules NCAM and N-Cadherin

Hansen

Berezin

Bock

2008

Cell. Mol. Life Sci.

105

115

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Formation of appropriate neural circuits depends on a complex interplay between extracellular guiding cues and intracellular signaling events that result in alterations of cytoskeletal dynamics and a neurite growth response. Surface-expressed cell adhesion molecules (CAMs) interact with the surroundings via the extracellular domain and bind to the cytoskeleton via their intracellular domain. In addition, several CAMs induce signaling events via direct interactions with intracellular proteins or via interactions with cell surface receptors. Thus, CAMs are obvious candidates for transmitting extracellular guidance cues to intracellular events and thereby regulating neurite outgrowth. In this review, we focus on two CAMs, the neural cell adhesion molecule (NCAM) and N-cadherin, and their ability to mediate signaling associated with a neurite outgrowth response. In particular, we will focus on direct interaction between NCAM and N-cadherin with a number of intracellular partners, as well as on their interaction with the fibroblast growth factor receptor (FGFR).

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Growth cone interactions with purified cell and substrate adhesion molecules visualized by interference reflection microscopy

Cited by 17 publications

References 49 publications

In vitro analyses of interactions between olfactory receptor growth cones and glial cells that mediate axon sorting and glomerulus formation

In vitro analyses of interactions between olfactory receptor growth cones and glial cells that mediate axon sorting and glomerulus formation

Quantitative Reflection Interference Contrast Microscopy (RICM) in Soft Matter and Cell Adhesion

Signaling mechanisms of neurite outgrowth induced by the cell adhesion molecules NCAM and N-Cadherin

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