Growth Characteristics of Microorganisms in Solid State Fermentation for Upgrading of Protein Values of Lignocelluloses and Cellulase Production

Chahal, D. S.

doi:10.1021/bk-1983-0207.ch020

Cited by 30 publications

(16 citation statements)

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“…Pretreatment of solid substrate with different chemicals act as scouring, sequestering and bleaching agent to enhance the crystallinity. It enhances the production rate of cellulases as it breaks lignin and carbohydrate bonds for successful degradation of cellulosic biomass by microorganisms 14 .…”

Section: Resultsmentioning

confidence: 99%

Sugar cane bagasse pretreatment: An attempt to enhance the production potential of cellulases by <i>Humicola insolens</i> TAS-13

ul-Haq¹,

Javed²,

Khan³

2010

Biokemistri

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Pretreatment of the cellulosic substrate has miracle effect on the enhancement of cellulase production by fungal strains. A thermophilic strain of Humicola insolens TAS-13 was locally isolated and was tested for cellulases production under solid-state fermentation conditions using sugar cane bagasse as substrate. The cultural conditions for the H. insolens were also optimized for the higher rate of cellulase secretion. In order to enhance the production rate of heterogenous cellulosic proteins, bagasse was pretreated with NaOH, H 2 SO 4 , H 2 O 2 and H 2 O 2 +1.5%NaOH. The pretreatment of bagasse with 2.0% H 2 O 2 along with 1.5% NaOH enhanced the biosynthesis of cellulases by H. insolens. Production rate was also optimized with different parameters like thickness of fermentation medium, initial pH, incubation time and temperature. The thickness of the fermentation medium of 0.8 cm (10 g) with pH range of 5.5 was found to be better for enhanced production at 50°C. The yield of the enzyme was reached maximum with CMC-ase (18.98 U/g/min), FP-ase (13.63 U/g/min), -glucosidase (19.54 U/g/min) 72 h after inoculation.

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Section: Resultsmentioning

confidence: 99%

Sugar cane bagasse pretreatment: An attempt to enhance the production potential of cellulases by <i>Humicola insolens</i> TAS-13

ul-Haq¹,

Javed²,

Khan³

2010

Biokemistri

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“…In solid-state fermentations, aeration has essentially two functions (Chahal, 1983;Tabak and Cooke, 1968): (1) oxygen supply for aerobic metabolism and (2) removal of CO 2 , heat, water vapor, and volatile components produced during the metabolism.…”

Section: Aerationmentioning

confidence: 99%

“…For these reasons, solid-state fermentations need a large quan-tity of air, exceeding the amount necessary for microbial respiration. At a semiindustrial scale, the limitation in cooling technique in solid-state fermentations enhances the use of thermophilic strains, as realized by numerous workers (Chahal, 1983;Moo-Young et at., 1977;Viesturs et at., 1981).…”

Section: Temperaturementioning

confidence: 99%

Solid-State Fermentations of the Genus Aspergillus

Gervais

Bensoussan

1994

Aspergillus

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“…First, the water is needed for the hydrolysis of the starch; this represents 1 mol of water consumed per mol of glucose produced, i.e., 0.018 g H,O/g DM at the end of the growth ( t = 160 h). Secondly, the water needed for the synthesis of fresh biomass may be estimated from equation (2) and is close to 0.31 g H,O/g DM at f = 160 h. The sum of these two terms is equal to…”

Section: B Time Course Of the Biomass Productionmentioning

confidence: 99%

Spore production of Penicillium roqueforti by solid state fermentation: Stoichiometry, growth and sporulation behavior

Desfarges¹,

Larroche²,

Gros³

1987

Biotech & Bioengineering

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The development of Penicillium roqueforti on buckwheat seeds proceeds roughly into four steps, involving a lag phase and three growth phases. First, it appears as a spore germination and external colonization of the grains by the mycelium. Then, mainly external sporulation and internal colonization of the seeds occur and finally internal sporulation takes place. The Stoichiometry of the growth and the sporulation is established. Kinetic experiments performed in a fixed bed reactor show that the growth of the microorganism (biomass production) may be estimated by the protein content of the medium. This growth occurs with a very low mu(max) value close to 0.030 h(-1). The chitin content of the medium is an indicator of the sporulation, just as the metabolic liquor (mainly water) produced during the course of a cultivation. The values of the observed respiratory quotient are close to those predicted by stoichiometry.

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Growth Characteristics of Microorganisms in Solid State Fermentation for Upgrading of Protein Values of Lignocelluloses and Cellulase Production

Cited by 30 publications

References 0 publications

Sugar cane bagasse pretreatment: An attempt to enhance the production potential of cellulases by <i>Humicola insolens</i> TAS-13

Sugar cane bagasse pretreatment: An attempt to enhance the production potential of cellulases by <i>Humicola insolens</i> TAS-13

Solid-State Fermentations of the Genus Aspergillus

Spore production of Penicillium roqueforti by solid state fermentation: Stoichiometry, growth and sporulation behavior

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