Growth Characteristics and Viral Susceptibility of a Chimpanzee (Pan troglodytes) Lung Diploid Cell Line, SFRE:CL-1

Chemically defined media SFRE-199-1 for the growth and SFRE-199-2 for the maintenance of primary baboon kidney (Bak) cell cultures were formulated by supplementing medium M199 with insulin, sodium pyruvate, zinc sulfate, and increasing arginine-HCl, cysteine, cystine, L-glutamine, L-glutamatic acid, glycine, histidine, tyrosine, and glucose to maximally active nontoxic concentrations. For prolonged maintenance of the cells, physiological pH control, and blocking of excessive lactic acid accumulation in the spent medium of the cell cultures, it is necessary to supplement the medium containing Earle's balanced salts with D-(+)galactose. The cells grew and were maintained equally well on glass or polystyrene surfaces. Selenium, when added to growth medium or substituted for insulin and zinc sulfate, did not stimulate cell growth. Electron microscopy showed that numerous dense particles, approximately 250 to 400 A in diameter, with the appearance of glycogen, were found throughout the cytoplasm in the cells grown in SFRE-199-1 and maintained in SFRE-199-2. Echovirus types 1 to 3, poliovirus types 1 to 3, coxsackievirus types B2, B4, B5, Herpes-virus hominis type 1, simian herpesvirus H. simiae and SA8, and simian adenovirus SV34 when titrated in primary Bak cells and grown and maintained in SFRE-199-1 and 2, respectively, developed titers comparable to those obtained in conventionally grown and maintained cells.

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Chemically defined serum-free media for the cultivation of primary cells and their susceptibility to viruses

Weiss

Lester

Kalter

et al. 1980

In Vitro

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Oncornavirus: Isolation From a Squirrel Monkey ( Saimiri sciureus ) Lung Culture

Heberling

Barker

Kalter

et al. 1977

Science

Self Cite

107

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An oncornavirus isolated from a squirrel monkey (Saimiri sciureus) lung culture has a density of 1.16 to 1.17 grams per milliliter, contains 70S RNA, and has an RNA-directed DNA polymerase that prefers Mg2+ over Mn2+ in an assay in which polyribocytidylate - oligodeoxyguanylate (12-18) is used as a synthetic template. Morphologically, the virus resembles Mason-Pfizer monkey virus but is antigenically distinct from this virus. The virus grows in cells of human, chimpanzee, rhesus monkey, canine, and mink origin, but not cells of squirrel monkey origin. On the basis of its properties, the newly isolated virus can be classified as a retravirus.

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Growth Characteristics and Viral Susceptibility of a Chimpanzee (Pan troglodytes) Lung Diploid Cell Line, SFRE:CL-1

Cited by 2 publications

References 4 publications

Chemically defined serum-free media for the cultivation of primary cells and their susceptibility to viruses

Chemically defined serum-free media for the cultivation of primary cells and their susceptibility to viruses

Oncornavirus: Isolation From a Squirrel Monkey ( Saimiri sciureus ) Lung Culture

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