Growth Characteristics and Biological Activities in Astragalus membranaceus Seedlings of Exposed to Different Types of Artificial Light

Seo, Ji Won; Lee, Jae Geun; Yoo, Ji Hye; Lim, Jung Dae; Kim, Myong Jo; Seong, Eun Soo

doi:10.7783/kjmcs.2022.30.5.339

Cited by 3 publications

(2 citation statements)

References 13 publications

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“…Accordingly, we speculate that our observations for A. membranaceus might also apply to other species in the family Leguminosae. In addition, it has also been reported that exposure to white LED light enhances the antioxidant activity of A. membranaceus [18]. Thus, we assume that the thickening of the stems of A. membranaceus grown under white LED light contributes to enhancing stress resistance and antioxidant activity.…”

Section: Discussionsupporting

confidence: 53%

“…At the cellular level, we found the in vitro-germinated A. membranaceus sprouts that had been exposed to red LED light were characterized by the largest leaf cells, whereas the thickest stem cells were observed in plants cultivated under white LED illumination (Table 1). Previous studies on the effects of different LEDs on Astragalus growth have found that the growth of A. membranaceus plants cultivated under blue LED illumination was superior to that of plants exposed to either white LED or red LED light when visually measured [18], although these authors detected no significant differences among plants treated with these three light sources with respect to the length and number of leaves. In studies on the effects of LED illumination on the growth of other plants, it has been shown that in Myrtus communis, the highest shoot multiplication effect occurred in plants treated with 5 µM BA(Benzyladenine) grown under red LED light [19], whereas, in Salvia miltiorrhiza, Choi et al (2020) found red LED light to be more effective than blue LEDs in promoting leaf length and width [20].…”

Section: Discussionmentioning

confidence: 85%

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Cellular Morphology and Transcriptome Comparative Analysis of Astragalus membranaceus Bunge Sprouts Cultured In Vitro under Different LED Light

Seo

Lee²,

Yoo

et al. 2023

Plants

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Astragalus membranaceus, the major components of which are saponins, flavonoids, and polysaccharides, has been established to have excellent pharmacological activity. After ginseng, it is the second most used medicinal plant. To examine the utility of A. membranaceus as a sprout crop for plant factory cultivation, we sought to establish a functional substance control model by comparing the transcriptomes of sprouts grown in sterile, in vitro culture using LED light sources. Having sown the seeds of A. membranaceus, these were exposed to white LED light (continuous spectrum), red LED light (632 nm, 1.58 μmol/m2/s), or blue LED light (465 nm, 1.44 μmol/m2/s) and grown for 6 weeks; after which, the samples were collected for transcriptome analysis. Scanning electron microscopy analysis of cell morphology in plants exposed to the three light sources revealed that leaf cell size was largest in those plants exposed to red light, where the thickest stem was observed in plants exposed to white light. The total number of genes in A. membranaceus spouts determined via de novo assembly was 45,667. Analysis of differentially expressed genes revealed that for the comparisons of blue LED vs. red LED, blue LED vs. white LED, and red LED vs. white LED, the numbers of upregulated genes were 132, 148, and 144, respectively. Binding, DNA integration, transport, phosphorylation, DNA biosynthetic process, membrane, and plant-type secondary cell wall biogenesis were the most enriched in the comparative analysis of blue LED vs. red LED, whereas Binding, RNA-templated DNA biosynthetic process, DNA metabolic process, and DNA integration were the most enriched in the comparative analysis of blue vs. white LED, and DNA integration and resolution of meiotic recombination intermediates were the most enrichment in the comparison between red LED vs. white LED. The GO term associated with flavonoid biosynthesis, implying the functionality of A. membranaceus, was the flavonoid biosynthetic process, which was enriched in the white LED vs. red LED comparison. The findings of this study thus indicate that different LED light sources can differentially influence the transcriptome expression pattern of A. membranaceus sprouts, which can provide a basis for establishing a flavonoid biosynthesis regulation model and thus, the cultivation of high-functional Astragalus sprouts.

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Section: Discussionsupporting

confidence: 53%

Section: Discussionmentioning

confidence: 85%

Cellular Morphology and Transcriptome Comparative Analysis of Astragalus membranaceus Bunge Sprouts Cultured In Vitro under Different LED Light

Seo

Lee²,

Yoo

et al. 2023

Plants

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Evaluation of Growth, Vegetation Indices, and Photosynthesis of Cichorium intybus L. Seedlings as Affected by LED Light Qualities in a Closed Nursery Facility

Shin,

Lee,

Nam

2024

HST

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Cichorium intybus, a leafy vegetable belonging to the Asteraceae family, is known for its potential health benefits and the inclusion of various bioactive compounds and indigestible oligosaccharides that improve liver function and aid in fatigue recovery. In this study, we evaluated the effects of light-emitting diode (LED) light qualities on the growth, vegetation indices, and photosynthesis of the C. intybus cultivars 'Asia Sugar' and 'Rossa Italiana', grown in a closed nursery facility. LED light treatments included seven types of monochromatic, composite, and white LEDs (i.e., red, green, blue; purple; 3000, 4100, and 6500 K white LEDs) applied to these cultivars. The results showed that 'Asia Sugar' reached its largest size under the 3000 and 4100 K white LED treatments, whereas 'Rossa Italiana' exhibited the highest growth under the 3000 K white LED light treatment. An analysis of monochromatic light revealed that red LED light increased plant sizes and weights compared to blue LED light, and a higher distribution of red wavelengths within the white light spectrum positively influenced plant sizes and weights. With regard to increasing plant weights, both cultivars performed best under 3000 and 4100 K white LEDs, similar to the results for plant sizes. In contrast, 'Asia Sugar' showed the most favorable vegetation and photosynthetic indices under 4100 and 6500 K white LEDs, while the 'Rossa Italiana' showed the most favorable photosynthetic indices and chlorophyll content (SPAD units) under 6500 K white LED, opposite to the plant size results. These findings provide valuable insights into how specific LED light qualities can be optimized to enhance the growth and physiological responses of Cichorium intybus cultivars seedlings.

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Differences in Growth Characteristics and Photochemical Responses of Salvia miltiorrhiza Bunge under Different LED Light Qualities in a Closed Plant Factory System

Kim,

Lee,

Nam

2024

KJMCS

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Growth Characteristics and Biological Activities in Astragalus membranaceus Seedlings of Exposed to Different Types of Artificial Light

Cited by 3 publications

References 13 publications

Cellular Morphology and Transcriptome Comparative Analysis of Astragalus membranaceus Bunge Sprouts Cultured In Vitro under Different LED Light

Cellular Morphology and Transcriptome Comparative Analysis of Astragalus membranaceus Bunge Sprouts Cultured In Vitro under Different LED Light

Evaluation of Growth, Vegetation Indices, and Photosynthesis of Cichorium intybus L. Seedlings as Affected by LED Light Qualities in a Closed Nursery Facility

Differences in Growth Characteristics and Photochemical Responses of Salvia miltiorrhiza Bunge under Different LED Light Qualities in a Closed Plant Factory System

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